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    Item type:Publication,
    Evaluation of bioactivities of Delonix regia extracts from different regions of Thailand
    (2018-01-01)
    This research is to study bioactivity of Delonix regia flower and leaf extracts collected in Thailand. We analyzed for efficacy of free radical scavenging and found that flower and leaf extracts from Samut Prakan showed the strongest percentage of reduction with IC50 50.21 and 105.0 mg/ml, respectively. The result of evaluated phytochemicals revealed that Chon Buri's flower extract had the highest of total phenolic and tannin contents at 200 mg GAE/g extract and 32 mg TAE/g extract, respectively. Furthermore, Samut Prakan's flower extract had the highest flavonoid compounds at 328.5 mg QE/g extract. To examine cytotoxicity on breast cancer cell lines, we found that Samut Prakan's flower extract had the most cytotoxicity CC50 value, 5000 µg/ml. In conclusion, we found that the flower of Delonix regia is the promising part for the further study because the antioxidant activity and cytotoxicity strongly enhances when flower extract concentration is increased.
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    Item type:Publication,
    In vitro fermentation, digestibility and methane production as influenced by Delonix regia seed meal containing tannins and saponins
    (2017-01-01)
    Supapong, C.
    ;
    Cherdthong, A.
    ;
    Seankamsorn, A.
    ;
    Khonkhaeng, B.
    ;
    Wanapat, M.
    The aim of the study was to evaluate the effect of supplementation of Delonix regia (DR) seed meal containing tannins and saponins on gas kinetics, ammonia-nitrogen (NH3-N) content, pH, methane (CH4) production and dry matter (DM) digestibility using an in vitro gas production technique. The experimental design was completely randomized, and the dietary treatments included DR seed meal supplementation at levels of 0, 3.3, 5.0, 6.7, 8.3, 10, 11.7, 13.3, 15.0 and 16.7 mg DM added to 0.5 g of roughage and concentrate (70:30) mixture. The gas production was measured at several time points: 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h by a pressure transducer. The parameters of gas kinetics and cumulative gas production were not altered (P > 0.05), except for gas production rate constant for the insoluble fraction which was the highest at 11.7 mg DR seed meal inclusion. CH4 production and total protozoa counts linearly decreased with increasing DR seed meal levels (P < 0.05). DR seed meal addition caused quadratic increase of in vitro DM digestibility with the highest value at 11.7 mg DR seed meal inclusion. No significant difference in volatile fatty acid profile (P > 0.05) was stated between treatments except for propionic acid. In conclusion, supplementation of DR seed meal resulted in improved in vitro gas kinetics and DM digestibility up to 11.7 mg level, while CH4 production was reduced linearly. The further in vivo studies are necessary to examine practical of DR seed meal usage in animal production.