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Subject: search.filters.subject.Hydroponics

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    Morphological and Molecular Identification of Pythium spp. from Hydroponically-Grown Lettuce
    (2022-01-01)
    Talubnak, Chulalak
    ;
    Schoonbeek, Henk Jan
    ;
    Parinthawong, Nonglak
    ;
    Jaenaksorn, Tanimnun
    Herein, hydroponic Pythium communities in Thailand were investigated. The 38 Pythium isolates from asymptomatic and symptomatic lettuce roots were identified using morphological and molecular features. The data indicated that P. aphanidermatum and P. myriotylum were the predominant species. Regarding the rDNA-ITS study, all isolates were identified as P. aphanidermatum, P. myriotylum, P. deliense, or an unidentified Pythium species. The reconfirmation of the three unidentified Pythium isolates using the cytochrome oxidase subunit I (COI) gene in comparison to the representative isolates was achieved using P. aphanidermatum and P. myriotylum. The COI phylogenetic trees were similar to that of the ITS tree. Additionally, pathogenicity of the Pythium representative isolates to plant seeds was evaluated in a laboratory assay. The seedlings suffered serious symptoms from P. myriotylum SR31 infection, with 90-100% disease severity. The other isolates presented disease severity of less than 20% when compared with uninoculated control. This study provides a comprehensive identification of Pythium root rot in lettuce grown on hydroponics in Thailand and provides information on beneficial microorganisms and a resistance inducer in lettuce root rot.
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    Growth and yield of sweet corn cultivated in soil using third reused nutrient solution
    (2020-05-01)
    Boonnoi, N.
    ;
    Nuntagij, I.
    ;
    Koohakan, P.
    Nutrient solution from growing lettuce using Nutrient Film Technique (NFT) was drained from the substrate culture to grow Chinese kale in Dynamic Root Floating Technque (DRFT) before the third usage for growing melon. The third re-used nutrient solution (UNS) was drained from the melon growing substrate, diluted with tap water, and adjusted the concentration to 2.0 mS/cm2, pH 5.8 before irrigation on the sweet corn field. Control plants were grown under general management (manual watering and fertilizing with 15-15-15 fertilizer). Results indicated that growth parameters including shoot height, SPAD value and fresh weight per plant using the third UNS were not significantly different from the control. Yield parameters including number of ears, average weight per ear and average weight per plant using the third UNS also showed no significant differences from the control. Water consumption of sweet corn under treatment of the third UNS and the control were 35.55 and 108.23 L/plant, respectively resulted to reduced water consumption of up to 68.07% and no fertilizer was used in the UNS treatment. Thus, the UNS method reduced both water and fertilizer consumption, while minerals in the nutrient solution were completely utilized, leading to cost reduction and environmentally friendly waste production.
      2
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    Phytoalexin production of lettuce (Lactuca sativa L.) grown in hydroponics and its in vitro inhibitory effect on plant pathogenic fungi
    (2017-09-01)
    Talubnak, Chulalak
    ;
    Parinthawong, Nonglak
    ;
    Jaenaksorn, Tanimnun
    A series of experiments were conducted to investigate phytoalexin production from five varieties of lettuce in hydroponics using abiotic (2.5, 5% CuSO<inf>4</inf>; 0.5, 1% AgNO<inf>3</inf>) and biotic elicitors (non-pathogenic Pythium sp.) at different plant ages. It showed that phytoalexin was successfully induced in tested lettuce grown in hydroponics after elicitation with abiotic elicitors throughout the trial. Phytoalexin showed yellow fluorescent spot under 365 nm UV light with R<inf>f</inf> 0.45-0.48 and clear inhibition zone where Aspergillus niger failed to develop on TLC plate. For biotic elicitors, no yellow fluorescent spot on TLC plate was observed from tested lettuce varieties however inhibition zone at R<inf>f</inf> 0.9 was detected at 8-9 weeks from red oak, green oak, and red coral. Moreover, crude extract of lettuce elicited with abiotic elicitors possessed in vitro antifungal activity against C. gloeosporioides, C. lunata, F. oxysporum, and P. aphanidermatum probably due to phytoalexin (lettucenin A) in the extract.
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    Ontology-based nutrient solution control system for hydroponics
    (2011-12-01)
    Phutthisathian, Areeworn
    ;
    Pantasen, Nakulrad
    ;
    Maneerat, Noppadol
    This paper presents the Ontology-based Nutrient Solution Control System for Hydroponics with Protégé. We consider variables of electrical conductivity (EC), Potential of Hydrogen ion (pH), intensity of solution, species of plants and the relation of the device in the system for supporting to make the suitable decisions with the control system of hydroponic nutrient solution. © 2011 IEEE.