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Subject: search.filters.subject.Limnophila aromatica

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    Discovery and characterization of bioactive compounds from Limnophila aromatica: nevadensin and related flavonoids as potent antimicrobial agents
    (2025-08-01)
    Maswanna, Thanaporn
    ;
    Maneeruttanarungroj, Cherdsak
    Limnophila aromatica, a traditional medicinal plant, has been previously reported to possess notable antimicrobial properties. However, the specific bioactive constituents responsible for this activity remain largely unidentified. This study aimed to isolate, identify, and evaluate the antibacterial potential of compounds from a 100% ethanolic extract of L. aromatica. The crude ethanolic extract exhibited the highest antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) and was subsequently subjected to Diaion HP-20 column chromatography, followed by preparative HPLC. Seven major peaks were identified using UV-Vis spectra, LC-QTOF-MS, and NMR analyses. The antibacterial efficacy of these isolated compounds was assessed using disc diffusion and broth microdilution assays against Bacillus subtilis, S. aureus, MRSA, Escherichia coli, and Pseudomonas aeruginosa. The isolated compounds were identified as norethindrone acetate, isothymusin, nevadensin, gardenin B, 5,3’-dihydroxy-7,8,2’-trimethoxyisoflavone, jasmolin II, and oleanolic acid. Nevadensin, the predominant compound (79.55%), demonstrated potent bactericidal activity against B. subtilis, S. aureus, and MRSA. Jasmolin II and gardenin B also exhibited promising antibacterial effects. Although the disc diffusion assay was limited by compound diffusion, the broth microdilution method confirmed significant MIC values, ranging from 0.59 to 2.86 mg/ml while the MBC values ranged from 0.59 to 12.72 mg/ml. This study highlights nevadensin and other flavonoids from L. aromatica as potential candidates for the development of alternative antibacterial therapies, particularly against drug-resistant Gram-positive bacteria.
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    Antimicrobial, antioxidant and anticancer activities of Thai local vegetables
    (2009-12-01)
    Nanasombat, S.
    ;
    Teckchuen, N.
    Thai local vegetables (20 species) were screened for their antimicrobial and antioxidant activities. Methanolic extracts of Cassia siamea, Garcinia cowa, Limnophila aromatica and Polygonum odoratum exhibited strong antioxidant and antibacterial activities. The sensitive bacteria to these extracts were Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus. Among all plant extracts, the extract of P. odoratum had the highest phenolic content and antioxidant activity with EC <inf>50</inf> value of 315.35 μg extract/mg DPPH (2,2-diphenyl-1-picrylhydrazyl). These plant extracts were also tested for cytotoxicity against human oral epidermal carcinoma (KB), breast adenocarcinoma (MCF-7) and small cell lung carcinoma (NCI-H187). P. odoratum extract was moderately active against MCF-7 (IC <inf>50</inf> value of 6.01 μg/ml). Major types of active compounds in P. odoratum extract were identified by HPLC method. Flavonoids found in this plant were rutin, catechin, quercetin, kaempferol and isorhamnetin. Among these compounds, rutin was found in the highest amount (3.77% w/w dry extract). © 2009 Academic Journals.