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    Consecutive bacterial cellulose production by luffa sponge enmeshed with cellulose microfibrils of Acetobacter xylinum under continuous aeration
    (2021-01-01)
    Krusong, Warawut
    ;
    Pothimon, Ruttipron
    ;
    China, Salvatore La
    ;
    Thompson, Anthony Keith
    The bacterial cellulose production (BCP) process, using cellulose microfibrils (CM) of Acetobacter xylinum enmeshed on luffa sponge matrices (LSM) as LSM-CM starter, has been successfully developed where the LSM-CM production process can be recycled through consecutive cycles in limited dissolved oxygen (DO) under continuous aeration. In this study, incremental aeration rates impacted the consecutive cycles. Gluconic acid production, during the process, resulting in the reduction of BCP, was increasingly generated at high aeration from 0.28 to 0.34% at 3 L/min to 0.83–0.97% and 1.52–1.99% at 6 and 9 L/min after 7 d culture at 30 ± 2 °C. To compensate for the negative impact of aeration, 0.10 and 0.15% (w/v) carboxymethyl cellulose (CMC) was found to create a microenvironment for recycled LSM-CM at both high aeration (6 and 9 L/min, respectively). Under nine consecutive BCP cycles, acceptable BC yields (from 5.54 ± 0.5 to 5.89 ± 0.5 g/L) were associated with high biomass at 6 L/min aeration. These results confirm that LSM-CM, combined with CMC called as LSM-CM-CMC, created microenvironments low in DO under high aeration rates and that the recycled LSM-CM-CMC with aeration is an alternative, sustainable, economic process that could be applied for mass BCP.
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    Successful simulation of airflow in the microenvironment of an assembly automation machine and its implication
    (2014-01-01)
    Thongsri, Jatuporn
    ;
    Khaokom, Adisorn
    Today, the hard disk drive (HDD) industry is using assembly automation machine (AAM) to construct head stack assembly (HSA) from smaller parts. AAM needs to operate in a clean environment with very low particle counts. To achieve this end, fan filter Unit (FFU) is used to supply purified air into the environment by filtering out airborne particles from recirculating air. In this study, we investigated numerically the airflow induced by FFUs inside a microenvironment that houses an AAM in an HDD factory. The boundary conditions chosen for simulation were directly derived from the real ambient conditions in this HDD factory. We found that the FFUs not only filter out airborne particles from the air supplied into the microenvironment but also act as a particle blocker, pushing away the nearby particles in the air surrounding the openings of the microenvironment. The findings from this study can be applied to cases where other kinds of machinery need to be protected from airborne particles. © (2014) Trans Tech Publications, Switzerland.