KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Subject: search.filters.subject.PCR

Search Results

Now showing 1 - 3 of 3
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    The prevalence of capripoxvirus causing lumpy skin disease in beef cattle with no clinical signs on a well-managed cooperative farm
    (2025-03-01)
    Kaewhom, P.
    ;
    Poolsamran, P.
    ;
    Ard-Rugsa, T.
    ;
    Phromnoi, S.
    ;
    Thammakarn, C.
    Capripoxviruses were confidently detected in cattle, exhibiting a prevalence of 1.17% (9 out of 770 samples). The PCR products from the infected cattle consistently ranged from 1,000 to 1,500 base pairs, even at very low viral loads. Importantly, no significant differences in infection rates were found between the summer and rainy seasons (P > 0.05), and the prevalence was not associated with specific geographical regions or the timing of blood sample collection (P > 0.05). Notably, infected cattle displayed no clinical signs, such as skin nodules, emphasising that the disease can be present without causing visible harm. These findings strongly reinforce the necessity for stringent disease prevention measures in beef cattle production to effectively mitigate potential losses.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Wolbachia infection in leafhoppers and planthoppers: Diversity, density and geographic distribution in tropical rice agroecosystems
    (2015-06-01)
    Wiwatanaratanabutr, Itsanun
    Members of the genus Wolbachia are a group of endosymbiotic bacteria that infect a wide range of arthropods and cause reproductive alterations in their hosts. A survey of these endosymbionts in leafhoppers and planthoppers in rice agroecosystems from 20 provinces in 4 different geographic regions of Thailand was carried out during January 2012 to December 2013. Most species were collected from growing rice fields among 5 habitat types surveyed from the Central and the Northeast regions. Infections of Wolbachia were screened by using polymerase chain reaction with wsp, ftsZ, 16S rRNA and groE gene primers and distinguished between A and B supergroup using wsp primers. Species of 15 leafhoppers and 18 planthoppers were collected and identified with 1, 14, 2, 14, 1 and 1 sp. representing the family Aphrophoridae, Cicadellidae, Cixiidae, Delphacidae, Lophopidae and Meenoplidae, respectively. Wolbachia were detected in 1377 of 1849 individuals (74.5%) representing 17 of 33 species (51.5%) of leafhoppers and planthoppers. Wolbachia had not been previously reported from Oliarus sp., Pyrilla perpusilla, Sogatella kolophon and S. vibix. The relative densities of Wolbachia within each individual were determined using quantitative real-time PCR and the result showed that the small brown planthopper Laodelphax striatellus had the highest Wolbachia density. These findings indicated that Wolbachia are distributed throughout leafhoppers and planthoppers from Thailand, suggesting that Wolbachia dynamics in nature may be affected by ecological factors.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Simulation and experiment of microheater for a microfluidic polymerase chain reaction device
    (2004-12-01)
    Klaitabtim, Don
    ;
    Lomas, Tanom
    ;
    Wisitsora-At, Anurat
    ;
    Tuantranont, Adisorn
    In this paper, we describe the simulation and experimental results of electroplated microheater for microfluidic-base polymerase chain reaction (PCR) device. This work used finite element analysis to simulate the temperature characteristics of a PCR device. The device consists of a reaction chamber using polydimethysiloxane (PDMS) with encapsulated micrhearter made of a nickel resistor wire. Design of the microheater mask layout using Tanner L-edit. Microheater can be made by low cost electroplating process. The temperature in the experiment was shown heating and cooling rates were 0.9°C/s and 0.8°C/s respectively. The thermal distribution of the device was also studied through finite elements modeling then compare to the experimental results. © 2004IEEE.