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    Streptomyces zingiberis sp. nov., an endophytic actinobacterium isolated from the root tissue of Zingiber montanum
    (2023-06-01)
    Pansomsuay, Rawirat
    ;
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An endophytic actinobacterium, designated strain PLAI 1-29<sup>T</sup>, was isolated from the root tissue of Zingiber montanum collected from Pathum Thani province, Thailand. Strain PLAI 1-29<sup>T</sup> was characterized using a polyphasic taxonomic approach. It typically exhibited morphological and chemotaxonomic properties of the genus Streptomyces. Strain PLAI 1-29<sup>T</sup> produced a spiral spore chain on aerial mycelium and grew at 15–40 °C, pH 6–10 on International Streptomyces Project 2 agar. The maximum NaCl concentration for growth was 9% (w/v). Cells of strain PLAI 1-29<sup>T</sup> presented ll-diaminopimelic acid, arabinose, galactose and ribose. The detected phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphati-dylinositol and phosphatidylinositol mannoside. The major menaquinones were MK-9(H<inf>6</inf>) and MK-9(H<inf>8</inf>). The major cellular fatty acids were iso-C<inf>16:0</inf>, anteiso-C<inf>15:0</inf> and anteiso-C<inf>17:0</inf> . The genome-based taxonomic details revealed the assignment of strain PLAI 1-29<sup>T</sup> to the genus Streptomyces and exhibited low threshold values for the delineation of a novel species by average nucleotide identity–blast (84.0%), average amino acid identity (80.0%) and digital DNA–DNA hybridization (27.6%) with its closest type strain, Streptomyces xinghaiensis S187<sup>T</sup>. Furthermore, several differential physiological and biochemical characteristics were detected between strain PLAI 1-29<sup>T</sup> and the closest type strain. Based on the combined phenotypic and genomic features, strain PLAI 1-29<sup>T</sup> (=TBRC 7645<sup>T</sup>=NBRC 113170<sup>T</sup>) is considered to represent a new Streptomyces species, for which we propose the name Streptomyces zingiberis sp. nov.
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    Streptomyces salinarius sp. nov., an actinomycete isolated from solar saltern soil
    (2023-01-01)
    Klanbut, Khanungkan
    ;
    Rattanakavil, Thitiya
    ;
    Duangupama, Thitikorn
    ;
    Suriyachadkun, Chanwit
    ;
    Herron, Paul Robert
    An actinobacterium, designated strain SS06011<sup>T</sup>, was isolated from solar saltern soil collected from Samut Sakhon province, Thailand. The taxonomic position of this strain was established using the polyphasic taxonomic approach. The strain produced grey aerial spore mass on International Streptomyces Project 2 seawater agar that differentiated into spiral spore chains with rugose-surfaced spores. Strain SS06011<sup>T</sup> was found to have ll-diaminopimelic acid in the cell peptidoglycan. Whole-cell hydrolysates contained galactose, glucose and ribose. MK-9(H<inf>6</inf> ) and MK-9(H<inf>4</inf> ) were major menaquinones. The major cellular fatty acids comprised iso-C<inf>16: 0</inf>, anteiso-C<inf>15: 0</inf> and anteiso-C<inf>17: 0</inf> . Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyl-glycerol and phosphatidylinositol were detected in cells. These characteristics were coincident with the typical morphological and chemotaxonomic properties of the genus Streptomyces. The taxonomic affiliation at the genus level of this strain could also be confirmed by its 16S rRNA gene sequence data. Strain SS06011<sup>T</sup> showed the highest 16S rRNA gene sequence similarity to Streptomyces ardesiacus NRRL B-1773<sup>T</sup> (99.1 %), Streptomyces coelicoflavus NBRC 15399<sup>T</sup> (99.1 %) and Streptomyces hyderabadensis OU-40<sup>T</sup> (99.1 %). Digital DNA–DNA hybridization (dDDH), average nucleotide identity-blast (ANIb) and average amino acid identity (AAI) values between strain SS06011<sup>T</sup> and its closely related type strains, S. ardesiacus NBRC 15402<sup>T</sup>, S. coe-licoflavus NBRC 15399<sup>T</sup> and S. hyderabadensis JCM 17657<sup>T</sup>, were in the range of 45.4–48.4 % (for dDDH), 90.8–91.9 % (for ANIb) and 90.8–91.7 % (for AAI), respectively, which are lower than the cut-off criteria for species delineation. The DNA G+C content of genomic DNA was 71.9 mol%. With the differences in physiological, biochemical and genotypic data, strain SS06011<sup>T</sup> could be discriminated from its closest neighbours. Thus, strain SS06011<sup>T</sup> should be recognized as representing a novel species of the genus Streptomyces, for which the name Streptomyces salinarius sp. nov. is proposed. The type strain is SS06011<sup>T</sup> (=TBRC 9951<sup>T</sup>=NBRC 113998<sup>T</sup>).
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    Streptomyces acidipaludis sp. nov., an actinobacterium isolated from peat swamp forest soil
    (2022-01-01)
    Nammali, Achararak
    ;
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An actinobacterium strain PLK6-54<sup>T</sup> was isolated from Lankwai peat swamp forest soil collected from Yala province, Thailand. Strain PLK6-54<sup>T</sup> exhibited morphological and chemotaxonomic properties described for the genus Streptomyces. It formed a spiral spore chain directly on aerial mycelium. Growth was observed between 20 and 40 °C and at pH 5–8. The maximum NaCl for growth was 2% (w/v). ll-Diaminopimelic acid, arabinose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside were detected as the phospholipids. The major menaquinones were MK-10(H<inf>2</inf>) and MK-9(H<inf>6</inf>). The major cellular fatty acids were iso-C<inf>16:0</inf>, anteiso-C<inf>15:0</inf> and iso-C<inf>14:0</inf>. 16S rRNA gene sequence data supported the assignment of strain PLK6-54<sup>T</sup> to the genus Streptomyces and showed that Streptomyces rubidus NBRC 102073<sup>T</sup> (99.0%) was the closest relative. Moreover, the average nucleotide identity-blast (85.5%) and digital DNA–DNA hybridization (30.7%) values reported between strain PLK6-54<sup>T</sup> and its closest neighbour were below the threshold values for delineation of a novel species. Strain PLK6-54<sup>T</sup> could be distinguished from related validly described Streptomyces species by several phenotypic properties. The combination of genotypic and phenotypic data indicated that strain PLK6-54<sup>T</sup> is representative of a novel species of the genus Streptomyces. The name Streptomyces acidipaludis sp. nov. is proposed for strain PLK6-54<sup>T</sup>. The type strain is PLK6-54<sup>T</sup> (=TBRC 11250<sup>T</sup>=NBRC 114297<sup>T</sup>).