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Access type: UnknownAuthor: search.filters.author.Cheubong, Chehasan

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    A smartphone-integrated rapid and sensitive lateral flow test strip for quantitative detection of Fusarium spp. in maize samples
    (2025-05-01)
    Rotamporn, Saowalak
    ;
    Cheubong, Chehasan
    ;
    Sansenya, Sompong
    ;
    Jantra, Jongjit
    ;
    Teepoo, Siriwan
    In the world, maize is considered as one of the most valuable resources. Fusarium spp. are the primary soilborne plant-pathogenic fungi that can infect maize seeds and cause diseases that potentially decrease the production of crops. This study aims to develop a rapid and sensitive lateral flow test strip (LFTS) utilizing gold nanoparticles (AuNPs) for on-site detection of Fusarium spp. in maize samples. The smartphone-integrated LFTS enables the identification of Fusarium spp. within a concentration range of 0.2–1.5 nM and achieves a limit of detection (LOD) as low as 0.063 nM. The quantitative determination of the Fusarium spp. can be completed within 8 min. Maize samples were analyzed using both the LFTS and real-time polymerase chain reaction (real-time PCR). The LFTS demonstrated effective recovery in spiked samples, with values ranging from 80.1% to 101.1%. Furthermore, the real-time PCR results exhibited significant agreement with the LFTS results. Compared to the traditional real-time PCR technique, the proposed LFTS offers a sensitive, accurate, and rapid assay for the on-site detection of Fusarium spp. in maize samples.
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    Colorimetric test strip cassette readout with a smartphone for on-site and rapid screening test of carbamate pesticides in vegetables
    (2022-10-01)
    Rakkhun, Waraporn
    ;
    Jantra, Jongjit
    ;
    Cheubong, Chehasan
    ;
    Teepoo, Siriwan
    A test strip cassette integrated with a smartphone detector was developed for the colorimetric detection of carbamate pesticides in vegetables. The influence of various experimental conditions was examined for the detection of the optimum analytical performance of the test strip cassette. The test strip cassette detection of carbamate pesticides was carried out by using 140 mM of acetylthiocholine, 13 mM of 5,5′-dithiobis (2-nitrobenzoic acid), temperature at 37 °C and pH of 8.0. The developed test strip cassette platform is rapid within 5 min. Based on the inhibition effect of carbaryl on acetylcholinesterase activity, the inhibition effect of carbaryl was proportional to its concentration ranging from 1 to 50 mg/L with the detection limit of 0.30 mg/L. The limit of quantitative value was determined to be 1.0 mg/L. The spiking carbaryl, the recoveries were within 96–103% and the relative standard deviations were less than 1.56%. The analysis of carbamate pesticides residual in vegetable samples gave the results in good agreement with the most widely used commercial test kit and UPLC-MS/MS method. The developed test strip cassette required only 5 min for an assay. With the simple procedures and tiny size, it showed a great promising for the developed test strip to work on-site for carbamate pesticides detection in vegetables.