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Author: search.filters.author.Mathaweesansurn, ArjnarongSubject: search.filters.subject.Image processing

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    Simple and fast fabrication of microfluidic paper-based analytical device by contact stamping for multiple-point standard addition assay: Application to direct analysis of urinary creatinine
    (2020-04-01)
    Mathaweesansurn, Arjnarong
    ;
    Thongrod, Suthathip
    ;
    Khongkaew, Putthiporn
    ;
    Phechkrajang, Chutima Matayatsuk
    ;
    Wilairat, Prapin
    In this work, a microfluidic paper-based analytical device (μPAD) for simultaneous multiple-point standard addition assay was fabricated by rubber stamping the hydrophobic barrier pattern onto a laboratory filter paper. The μPAD has a central zone from which an applied sample flows into eight surrounding narrow channels to which had been added the standard solutions. Each channel is connected to a circular area loaded with the reagent. The opposite end of this reagent zone is connected by second narrow channel to the final circular detection zone. The μPAD was applied to the measurement of creatinine in human urine. After addition of a urine sample, the orange-colored product arising from the Jaffé reaction is formed at the eight detection zones. A digital image of the μPAD is then recorded and the ratio of the red/green (R/G) intensity obtained using the ImageJ™ program is used in the quantitation of creatinine. The normal standard addition calibration line is constructed using the intensity ratio against the added creatinine concentrations (50–1000 mg L<sup>−1</sup>). Good linearity was achieved (r<sup>2</sup> ˃ 0.99). There were no significant differences between the creatinine content using an HPLC method (paired t-test at 95% confidence, t<inf>stat</inf> = 1.78, t<inf>critical</inf> = 2.26, n = 10). The use of simultaneous multiple-point standard addition calibration allows rapid determination of creatinine in urine with elimination of matrix interference.
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    A design of colorimeter for chemical quantitative analysis based on a web-camera
    (2017-10-19)
    Phongern, Komsun
    ;
    Mathaweesansurn, Arjnarong
    ;
    Maneerat, Noppadol
    ;
    Chengchan, Nathawut
    This paper presents the design of colorimeter for chemical quantitative analysis based on image processing. It comprises of two main parts which are test box and software. The test box consists of a web camera and LED lamp plat which is used to illuminate a micro wells. The software is graphic user interface and image processing. The main of image processing are split RGB channel of each pit in micro wells so calculate calibration curves for finding and unknown concentric solution then show result on screen. The experiment shows the design in this paper can calculate concentric of the solution. The result of the experiment have a correlation of reaction between Fe (II) and Phenanthroline (Red product) 0.985, the reaction between Ethanol and Methyl orange (Yellow product) 0.933 and reaction between Uric acid and Phosphotungstic acid (Blue product) 0.986. Moreover it takes a less time and lower cast compared with the spectrophotometer. The device can be applied to chemical laboratory by possibility.
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    A mobile phone-based analyzer for quantitative determination of urinary albumin using self-calibration approach
    (2017-04-01)
    Mathaweesansurn, Arjnarong
    ;
    Maneerat, Noppadol
    ;
    Choengchan, Nathawut
    This work demonstrates use of a smart mobile phone installed with an Android application, termed ‘Albumin smart test’, as an analyzer for quantitative determination of urinary albumin. The reaction between albumin and tetrabromophenolphthalein ethyl ester (TBPE) in the presence of Triton X-100 was employed for detection principle.The mobile phone was exploited with the sample cassette and the test paper. One sample cassette composes of two holders for accommodation of control and test samples. The test paper was designed in order to contain standard colorimetric strip and space for situating the sample cassette. Optical images of the strip and the samples were simultaneously captured in a single shot by a digital camera of the mobile phone and were digitally processed by the developed application for quantification of the albumin concentration based on self-calibration approach. With the advantage of self-calibration, the albumin test by our mobile phone can be performed in ambient light without using any extra module integrated with lighting control device. The other advantages are portability, ease of implementation and rapid analysis (3 min) with high precision (RSD ≤ 2.5%) and high accuracy (Recovery = 98.7% ± 1.6). The mobile device was successfully applied to diagnosis of microalbuminuria.