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Automated Screening of Cervical Cancer Cell Images
(2019-01-10)
Sangworasil, Manas
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Sukkasem, Chayanisa
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Sasivimolkul, Suvicha
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Suvarnaphaet, Phitsini
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Pechprasarn, Suejit
Cervical cancer is the first-most common type of female cancer and the second leading cause of death in Thailand. The number of cervical cancer is increasing in every year, even though it is preventable by the screening in early detection. The most popular method for the screening is so-called Pap smear test via examining morphology change in cervix cells. The aim of this research is to implement an image processing algorithm for classifying Pap smear cell images by calculating nucleus-to-cytoplasm area ratio. The algorithm used to classify the nucleus was mathematically calculated through k-mean clustering. The cytoplasm area was calculated from its edge profile relating to geometrical rotation method. Finally, the abnormal cells can be segmented using the area of nucleus-to-cytoplasm ratio with the accuracy of detection at 79%.
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Medical image compression using vector quantization and system error compression
(2015-09-01)
Phanprasit, Tanasak
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Hamamoto, Kazuhiko
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Sangworasil, Manas
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Pintavirooj, Chuchart
A novel medical image compression scheme based on vector quantization (VQ) is proposed in this paper. The advantages of the technique are not only that it yields high compression ratio but also that it maintains a peak signal-to-noise ratio (PSNR). This new method involves three steps. First, we present a codebook design using discrete wavelet transform (DWT), fuzzy C-means (FCM), and support vector machine (SVM) algorithms. Second, we improve the bit rate using the Huffman coding theme as a method of eliminating the redundant index. Finally, we supplement the system with error compensation to improve the PSNR. With the proposed method, we are able to achieve a bit rate improvement of 24.00% and a PSNR of 10.96% over the conventional method.
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Real-time multianalyte biosensors based on interference-free multichannel monolithic quartz crystal microbalance
(2015-05-05)
Jaruwongrungsee, Kata
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Waiwijit, Uraiwan
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Wisitsoraat, Anurat
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Sangworasil, Manas
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Pintavirooj, Chuchart
In this work, we design, fabricate and characterize a new interference-free multichannel monolithic quartz crystal microbalance (MQCM) platform for bio-sensing applications. Firstly, interference due to thickness-shear vibration mode coupling between channels in MQCM array is effectively suppressed by interposing a polydimethylsiloxane wall between adjacent QCM electrodes on a quartz substrate to form inverted-mesa-like structure. In addition, the electrical coupling due to the electrical impedance of solution is diminished by extending the flow path between them with an extended-design flow channel. The electrical testing results show that individual QCM signal is unaffected by those of adjacent channels under liquid loading, signifying the achievement of interference-free MQCM. The MQCM is applied for multi-analyte biosensing of IgG and HSA. The anti-IgG and anti-HSA are separately immobilized on two adjacent QCM electrodes, which are subsequently blocked with BSA to avoid unspecific binding. The MQCM biosensors are tested with single- and double-analyte solutions under continuous flow of buffer. The IgG and HSA QCM sensors only show frequency shift responses to their corresponding analytes and there are very small cross frequency shifts due to remnant unspecific binding. Moreover, MQCM sensors show approximately linear frequency shift response with analyte concentration. Therefore, the developed MQCM platform is promising for real-time interference-free label-free detection and quantification of multiple bio-analytes.
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An accurate forearm EMG signal classification method using two-channel electrode
(2013-01-01)
Sueaseenak, Direk
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Chanwimalueang, Theerasak
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Pintavirooj, Chuchart
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Sangworasil, Manas
An accurate electromyography (EMG) classification algorithm to control a virtual hand prosthesis with 12 degrees of freedom using two surface EMG electrodes is presented in this paper. We propose the application of independent component analysis (ICA) for blind-source separation of the EMG signals obtained from two electrodes. One of the problems affecting the EMG classification accuracy is the location dependence of the EMG signal due to the superposition of signals from multiple sources. ICA is used to separate the two signals obtained from two surface electrodes into two independent EMG signals prior to the feature extraction and classification processes. We demonstrate that the EMG classification accuracy can be improved using the ICA algorithm. We also propose a novel eigen-based feature that is extracted from the short-time Fourier transform (STFT) magnitude spectrum. Our new feature not only decreases feature dimensions but also performs better than other well-known features. We also implement the EMG classification scheme on the virtual robot arm. The performance shows promising result as indicated by a decrease in the Davies-Bolden (DB) index after applying the ICA. © 2013 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
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Real-time identification of electromyographic signals from hand movement
(2012-10-02)
Sappat, Assawapong
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Mahaphonchaikul, Kritsanaphan
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Sangworasil, Manas
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Pintavirooj, Chuchat
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Tuantranont, Adisorn
In this work, we have demonstrated a novel on-line technology for real-time acquisition and identification of electromyographic (EMG) signals from hand movement. EMG signal were measured using standard surface electrodes from forearm muscles at three major points including Wrist extensor, Flexor Carpi Radialis and Wrist Flexor groups, respectively. The EMG acquisition system consists of an instrumentation amplifier, filter circuit, isolator, an amplifier with gain adjustment and a commercial embedded system called FiO board. The commercial FiO embedded system is interfaced with the computer and EMG is represented, analyzed and stored in real-time on computer by Simulink program. EMG signals are identified by RMS and SD feature extraction methods and k-mean clustering algorithm. The result revealed that both RMS and SD can be used with k-mean clustering algorithm to obtain the distinct Euclidean distance characteristic of EMG signal for each movement. The minimum Euclidean distance with RMS and SD for each hand movement uniquely occurs at a distinct Euclidean distances between real EMG data and extracted features. © 2012 IEEE.
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On-chip cell separation and manipulation using traveling wave dielectrophoretic force controlled by four-phase signal generator
(2011-12-01)
Jaruwongrangsee, Kata
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Maturos, Thitima
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Bunthawin, Sakshin
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Wisitsoraat, Anurat
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Sangworasil, Manas
In this work, we present a device for cell manipulation and separation using travelling wave dielectophoretic (twDEP) force. The device consists of microchamber and 16 parallel electrode array controlled by four-phase signal. The dielectrophoretic PDMS chamber and Cr/Au parallel electrode array were fabricated by standard microfabrication techniques. The driving signals for the twDEP system are produced by a specially designed four-phase signal generator. The signal generator can be operated in a fixed or±90° phase shift mode with varying frequency, applied voltage and shift interval. In addition, the signal conditions can be store to and retrieved from a built-in database. The twDEP system was tested with polystyrene microspheres suspension in de-ionized water and red blood cells in D-mannitol solution. Cells responded to the electric field in various mechanisms depending on the applied signals conditions. The results showed that the twDEP force occurred when the applied signals were 10 V (50 kHz-700 kHz) and 7V (30 kHz-1MHz) for 4.5 μm and 10 μm microspheres respectively. The mixed solution containing equal amount of 4.5 and 10 μm microspheres were used for separation test. Under the signal conditions for 10 μm microspheres, the microspheres were moved under twDEP force while the smaller microspheres were attached to the electrodes. Therefore, the twDEP device can successfully manipulate and separate the microspheres of different sizes, and it can be further applied for cells selection. © 2011 IEEE.
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High-sensitivity humidity sensor utilizing PEDOT/PSS printed quartz crystal microbalance
(2011-08-12)
Jaruwongrungsee, Kata
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Sriprachuabwong, Chakrit
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Sappat, Assawapong
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Wisitsoraat, Anurat
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Phasukkit, Pattarapong
In this work, quartz crystal microbalance humidity sensor was fabricated by inkjet printing technique. Poly (3, 4-ethylenedioxythiophene)/poly-styrene- sulfonic acid (PEDOT/PSS), one of the most widely used polymer composites, was printed on QCM electrode as sensing layer using Dimatrix material inkjet printer. The main advantage of this coating method is its high precision of solution coating with accurately controlled volume and area. The printed layer was varied from 1 to 20 layers. With 20 PEDOT/PSS printed layers, the humidity sensitivity is found increased by more than three orders of magnitude compared to uncoated QCM. In addition, the PEDOT/PSS coated QCM exhibits fast humidity detection with short response and recovery times. Thus, the PEDOT/PSS printed on the QCM electrode is an effective way to improve humidity-sensing characteristic of QCM. © 2011 IEEE.
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Flow simulation in coronary artery models: An investigation of the effect of variable angulations at the left coronary artery
(2010-12-01)
Chaichana, Thanapong
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Sun, Zhonghua
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Tungjitkusolmun, Supan
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Sangworasil, Manas
This study was designed to investigate the hemodynamics in the left coronary artery with the aim of identifying the relationship between angulations of left coronary bifurcation and development of atherosclerosis. Six 3D left coronary models were simulated for computational fluid dynamic analysis. The left coronary model was composed of left main stem, left anterior descending and left circumflex branches. The angulations at the left bifurcation were simulated with angles ranging from 90°, 75°, 60°, 45°, 30° to 15°. The computational fluid dynamic was used for analysis of flow velocity, wall pressure and wall shear stress. Our results showed that apparent low shear stress and high wall pressure was noticed at the left coronary bifurcation regions with wide angled models. Flow pattern was also changed with angled becoming wide in the simulated models. Our analysis shows direction relationship between coronary angulation and development of atherosclerosis. Future studies are required to perform computational fluid dynamic analysis in coronary models from patients' data with different degree of coronary stenosis. © 2010 IEEE.
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Design and simulation of flow cell chamber for quartz crystal microbalance sensor array
(2010-07-30)
Jaruwongrungsee, Kata
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Maturos, Thitima
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Sritongkum, Pornpimol
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Wisitsora-At, Anurat
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Pintavirooj, Chuchart
In this paper, the cause of significant variation in sensing responses of identical QCM sensors array in a circular QCM chamber is analyzed by fluid dynamic simulation. Moreover, the simulation is used to determine an appropriate shape of flow chamber for QCM sensor array. Simulation results show that the flow in a circular-shaped QCM chamber design is primarily turbulent. In addition, the degree of turbulence is increased with flow rate. Thus, sensors at various locations see different sample dispersions causing their sensing behaviors to be significantly different. The QCM chamber has been redesigned to be rectangular line and simulation results indicate that it can be the solution to the problem because flow in the new design is primarily laminar with uniform sample velocity.
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A design of embedded DMX512 controller using FPGA and XILKernel
(2009-12-16)
Yoowattana, Sarat
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Nantajiwakornchai, Chinnapat
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Sangworasil, Manas
A design of the various control devices with a DMX512 Protocol is widely used in many areas, such as decorative lighting for both inside and outside of the building, or stage lighting and equipment control, such as spot light, smoke machine, strobe light, moving light. This article presents the creation of Embedded DMX512 Controller using FPGA with Real Time Operating System (RTOS). This creation enables us to build multiple channels of DMX Controller within a single device and to control DMX Controller through the TCP/IP. Thus we can control or update the data through the internet. © 2009 IEEE.

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