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    Properties of different varieties of durian
    (2021-06-02)
    Arsa, Supeeraya
    ;
    Wipatanawin, Angkana
    ;
    Suwapanich, Rachit
    ;
    Makkerdchoo, Orachorn
    ;
    Chatsuwan, Niphattha
    Durian (Durio zibethinus Murr.), like many other exotic, tropical, and conventional fruits, is important in the prevention of different diseases. In this study, the characterization of the main bioactive compounds of the most popular cultivars of durian and their properties are described. The changes in the quality indices of the antioxidant status were determined by CUPRAC, ABTS, FRAP, DPPH, and ORAC assays. The profiling of phytochemicals was carried out by Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). For the first time, in vitro studies were performed by the interaction of extracted durian polyphenols with human serum proteins (HSP) such as human serum albumin (HSAlb), fibrinogen (HSFib) and globulin (HSGlo) as novel biomarkers of coronary artery disease (CAD). The fluorescence measurements of the resulting intensity and calculated binding properties of the interaction of polyphenols with proteins showed that the most reactive was Monthong durian cultivar. This study suggests that durian cultivars have relatively strong antioxidant, binding, and health potentials and could be a significant source of natural antioxidants used in daily fresh consumption and for functional foods.
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    A mobile phone-based analyzer for quantitative determination of urinary albumin using self-calibration approach
    (2017-04-01)
    Mathaweesansurn, Arjnarong
    ;
    Maneerat, Noppadol
    ;
    Choengchan, Nathawut
    This work demonstrates use of a smart mobile phone installed with an Android application, termed ‘Albumin smart test’, as an analyzer for quantitative determination of urinary albumin. The reaction between albumin and tetrabromophenolphthalein ethyl ester (TBPE) in the presence of Triton X-100 was employed for detection principle.The mobile phone was exploited with the sample cassette and the test paper. One sample cassette composes of two holders for accommodation of control and test samples. The test paper was designed in order to contain standard colorimetric strip and space for situating the sample cassette. Optical images of the strip and the samples were simultaneously captured in a single shot by a digital camera of the mobile phone and were digitally processed by the developed application for quantification of the albumin concentration based on self-calibration approach. With the advantage of self-calibration, the albumin test by our mobile phone can be performed in ambient light without using any extra module integrated with lighting control device. The other advantages are portability, ease of implementation and rapid analysis (3 min) with high precision (RSD ≤ 2.5%) and high accuracy (Recovery = 98.7% ± 1.6). The mobile device was successfully applied to diagnosis of microalbuminuria.