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Subject: search.filters.subject.Colon cancer

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    Sulfated polysaccharides derived from marine microalgae, Synechococcus sp. VDW, inhibit the human colon cancer cell line Caco-2 by promoting cell apoptosis via the JNK and p38 MAPK signaling pathway
    (2023-01-01)
    Srimongkol, Piroonporn
    ;
    Songserm, Pajareeya
    ;
    Kuptawach, Kittisak
    ;
    Puthong, Songchan
    ;
    Sangtanoo, Papassara
    This study offers an examination of the physicochemical qualities of marine microalgae-derived sulfated polysaccharides (MMPS) from Synechococcus sp. VDW along with an investigation of their antioxidant and antitumor activities. On average, MMPS offered a molecular weight of 190.94 kDa with sulfate content of 16.83 %. There were shown to be four different monosaccharides contained within MMPS. The anticancer characteristics of human colon cancer cell lines (Caco-2) were also investigated. Flow cytometry revealed that MMPS triggered apoptosis at doses of 1 mg mL<sup>−1</sup>. Meanwhile, analysis of gene and apoptosis protein expression demonstrated that MMPS increased apoptosis in Caco-2 cells via the p38 MAPK and JNK signaling pathways. These findings contribute to our current understanding of how MMPS helps promote tumor suppression. In conclusion, the possibility that MMPS may possess anticancer qualities in the treatment of human colon cancer makes it an appealing candidate for anticancer polysaccharide combinations with chemotherapeutic agents and functional foods.
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    Cytotoxic activity and apoptotic induction of some edible Thai local plant extracts against colon and liver cancer cell lines
    (2017-12-01)
    Putthawan, Pornhathai
    ;
    Poeaim, Supattra
    ;
    Areekul, Varipat
    Purpose: To evaluate eight edible Thai local plant extracts (Camellia sinensis, Careya sphaerica, Cratoxylum formosum, Eleutherococcus trifoliatus, Ficus auriculata, Persicaria odorata, Schima wallichii, and Vaccinium sprengelii) against colon and liver cancer cell lines. Methods: The 80 % ethanol plant extracts were screened for cytotoxic activity against human colon adenocarcinoma (HT-29) and human hepatocellular carcinoma (HepG2) cells by MTT assay. The 50 % cytotoxic activity concentration (CC<inf>50</inf>) was then determined. Apoptotic cell death was observed by inverted microscopy and DNA fragmentation using agarose gel electrophoresis. Results: P. odorata and S. wallichii extracts showed strong cytotoxic activity, with the latter exhibiting more potent cytotoxic activity than the former. The CC<inf>50</inf> value of S. wallichii extract was 453 and 367 µg/mL against HT-29 and HepG2 cells, respectively. In contrast, P. odorata extract showed CC<inf>50</inf> value of 775 µg/mL against HT-29 and 1665 µg/mL against HepG2 cells. Microscopic observations indicate that the degree of morphological changes was concentration-dependent. The cell lines treated with both plant extracts displayed apoptosis. Conclusion: The two plant extracts have high potentials for medicinal use in colon and liver cancer management. However, further studies are needed to isolate the active compounds responsible for the cytotoxic activities.