KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Search Results

Now showing 1 - 9 of 9

Some of the metrics are blocked by your
consent settings
Extraction techniques, structural features, and functional properties of collagenous derivatives from unconventional animal sources: a review
(2026-12-01)
Indriani, Sylvia
;
Petcharat, Tanyamon
;
Andriani, Cynthia
;
Benjakul, Soottawat
;
Nalinanon, Sitthipong
Collagenous derivatives (collagen, gelatin, and collagenous hydrolysate (CH)) are extensively used across the food, biomedical, and pharmaceutical industries. Traditionally, these have been sourced from porcine, bovine, and fish due to their ready availability and biocompatibility. However, conventional collagenous derivatives face ongoing challenges regarding sustainability, resource intensity, and socio-cultural perceptions. This has led to the exploration of alternative collagenous derivatives from unconventional sources, with a primary focus on evaluating their potential for yields, extractability, and functional properties, all of which are fundamental for future scale-up and alternative applications. This review summarizes alternative collagenous derivatives from unconventional animals, including amphibians, mollusks, echinoderms, insects, unconventional fish and byproducts, and reptiles. Their structures, extraction techniques, functional properties, and potential applications are comprehensively summarized, showcasing their ability to complement or even surpass conventional sources in specific uses. Additionally, the challenges and prospects for industrial application, emphasizing the sustainability of meeting growing collagen demand and encouraging further research into these promising alternative sources, were discussed. Unconventional collagenous derivatives demonstrate excellent and unique characteristics as alternatives to conventional ones. Type I collagen from amphibians, reptiles, and mollusks had superior thermal stability. Unconventional gelatin and CH also possess various bio-functionalities that can enhance their potential applications. The relatively low extraction yield could be addressed by increasing the concentration of chemicals or extraction time and incorporating green technology without causing an adverse impact on the quality. These findings indicate the potential applications of unconventional collagenous derivatives as food ingredients and supplements.
Some of the metrics are blocked by your
consent settings
Sustainable Goat Skin Gelatin-Based Edible Coatings Incorporated with Konjac Glucomannan: Physicochemical Properties and Preservation Efficacy on Strawberries
(2026-04-01)
Hasdar, Muhamad
;
Nalinanon, Sitthipong
;
Nirmal, Nilesh Prakash
;
Petcharat, Tanyamon
;
Sriket, Chodsana
This study evaluated the effectiveness of edible coatings made from goat skin gelatin, with and without konjac glucomannan, for preserving strawberries at room temperature. Three groups were analyzed: a control group (EKG1), a gelatin coating group (EKG2), and a gelatin with konjac glucomannan group (EKG3). The addition of konjac glucomannan increased coating viscosity and thickness. While EKG3 initially raised total soluble solids, this difference disappeared by day 3. Both gelatin-based coatings maintained pH levels and showed no significant firmness difference between EKG2 and EKG3. The coatings effectively slowed color changes and pigment oxidation because they limited oxygen exposure. Weight loss was similar for EKG2 and EKG3, but EKG3 better preserved ascorbic acid on day 12. These results suggest that natural coatings can effectively reduce fruit spoilage, supporting sustainable food packaging and circular economy principles because they extend shelf life while being environmentally friendly.
Some of the metrics are blocked by your
consent settings
Optimization of Gelatin Fibrous Scaffold Properties by PCL and CMC by Using Electrospinning Technique
(2025-01-01)
Meesa, Banpot
;
Klongboonjit, Sakon
This study aimed to utilize the electrospinning process to produce cell culture scaffolds from blends of gelatin-polycaprolactone and carboxymethyl cellulose. The experimental design involved determining the optimal voltage and feed rate for various ratios of the gelatin-polycaprolactone-carboxymethyl cellulose blends, including 100/0/0, 90/5/5, 80/15/5, 70/25/5, and 60/35/5. Gelatin served as the primary raw material at a 10% ratio, while polycaprolactone was added at 10%, and carboxymethyl cellulose acted as a strengthening agent at 0.8%. The solvent used for gelatin and polycaprolactone was 2,2,2 -trifluoroethanol, while water was used for carboxymethyl cellulose. The raw materials were thoroughly mixed to ensure homogeneity, and the resulting blend was processed by an electrospinning machine under various conditions to form nanofiber scaffolds. The workpieces were then dried and left to relax for 48 hours before being baked at 140°C for 72 hours, resulting in high-quality fiber material. The experiment revealed that the fiber sizes ranged from 1.5 μm to 5.2 μm, with the swelling ratio of the GPC60:35:5 mixture at 11.65%, confirming the feasibility of using electrospinning to create effective scaffolds for cell culture applications.
Some of the metrics are blocked by your
consent settings
Electrospinning of Nanofibers Effect of Gelatin by Polycaprolactone and Carboxymethyl Cellulose Degradation Characteristics
(2025-01-01)
Meesa, Banpot
;
Klongboonjit, Sakon
This study aims to investigate the degradation of gelatin-based polycaprolactone and carboxymethyl cellulose scaffolds produced through the electrospinning technique for nanofiber scaffolds. The experimental design varies the voltage and feed rate for different ratios of gelatin, polycaprolactone, cellulose, and carboxymethyl cellulose, which are 100/0/0, 90/5/5, 80/15/5, 70/25/5, and 60/35/5, respectively. An organic solvent, 2,2,2-trifluoroethanol, which is a suitable solvent for gelatin, polycaprolactone, and carboxymethyl cellulose, is used, though the materials are dissolved in water to prepare the raw material for electrospinning. To characterize the scaffolds, their physical properties are analyzed, including fiber morphology and size, using scanning electron microscopy. The results reveal that as the polycaprolactone content increases from 0%, 5%, 15%, 25%, to 35%, with carboxymethyl cellulose maintained at 0% or 5%, the fiber size decreases from 1.5 μm to 5.2 μm. This suggests that electrospinning is effective for fabricating scaffolds from all three materials. Furthermore, the decomposition rates are optimized for GPC90/5/5, GPC80/15/5, and GPC70/25/5, which completely decompose within 36 hours. Additionally, GPC80/15/5 shows a good degradation rate, while GPC100/0/0 and GPC60/35/5 exhibit rapid degradation.
Some of the metrics are blocked by your
consent settings
Comprehensive Characterization of Gelatin Films from Goat Skin Incorporating Konjac Glucomannan: Physical, Mechanical, and Molecular Properties
(2024-12-01)
Hasdar, Muhamad
;
Nalinanon, Sitthipong
;
Kittiphattanabawon, Phanat
;
Sriket, Chodsana
This study aimed to comprehensively investigate and characterize the physical, mechanical, and molecular properties of gelatin films made from goat skin incorporated with konjac glucomannan. The study involved three treatment groups, labeled GG/KG1, GG/KG2, and GG/KG3, which included konjac glucomannan at concentrations of 0, 10, and 20% (w/w), respectively. Glycerol at 20% (w/w) was also included as a plasticizer. All samples underwent homogenization and ultrasonic treatment. The addition of konjac glucomannan to the gelatin-based film resulted in changes such as increased thickness (0.033-0.093 mm), opacity (0.9910-1.0433 mm-1), color L* (92.45-92.77), color difference (48.13-48.38), swelling (65.53-69.47%), and contact angle (86.92-127.85o). Conversely, a decrease was observed in water activity (0.521-0.463 Aw), moisture content (9.87-9.62%), tensile strength (0.0171-0.0118 N/mm2), elongation at break (5.91-4.52%), young’s modulus (0.0029-0.0026 N/mm²), WVTR (118.99-116.82 g/m². day), transparency (81.59-67.33%), and water resistance (34.47-30.53 %). Additionally, the peaks of amides A, B, I, II, and III exhibit both a shift and an increase in intensity, suggesting structural modifications and molecular interactions. The microstructure also indicated the presence of goat skin gelatin and konjac glucomannan cross-linked in the film formation. Therefore, the addition of konjac glucomannan modifies gelatin-based films, enhancing their suitability for food packaging.
Some of the metrics are blocked by your
consent settings
Impact of pretreatment with acid and ultrasound on the production and characteristics of goat skin gelatin
(2024-08-01)
Hasdar, Muhamad
;
Nalinanon, Sitthipong
;
Sriket, Chodsana
Goat skin is a potential raw material source for gelatin production using acid-ultrasound pretreatment. The objective of this study was to investigate the use of ultrasound in combination with acid pretreatment for the preparation of goat skin gelatin. Gelatin was extracted from goat skin using different pretreatments: Acetic acid (T1), acetic acid followed by ultrasound (20 kHz and 750 W) (T2), and without pretreatment (T0). The results showed that the combination of acetic acid and ultrasound pretreatment significantly impacted the quality of the resulting gelatin. The study results showed an increase in yield (9.24 to 25.48%), hydroxyproline content (102.07 to 231.31 mg/g), gel strength (4.76 to 197.62 g), viscosity (6.80 to 48.00 cP), melting point (32.47 to 35.85 °C), EAI (18.24 to 23.58 m<sup>2</sup>/g), and ESI (24.90 to 62.63 min). However, there was a decrease in pH, the value of color L*, and turbidity. The SDS-PAGE patterns showed differences in molecular weight distribution due to variations in pretreatment. All gelatin samples exhibited α<inf>1</inf> and α<inf>2</inf> chains as the predominant components. Interestingly, the ultrasound effect highlighted the β-chain more boldly compared to other pretreatments. FTIR spectroscopy analysis showed changes in molecular interactions due to acetic acid pretreatment followed by ultrasound, which resulted in shifts in the Amide A, Amide B, Amide I, Amide II, and Amide III groups. Ultrasonic treatment caused more dense and disturbed structures in the sample. Therefore, the combination of acetic acid and ultrasound pretreatment yielded the superior properties of goat skin gelatin.
Some of the metrics are blocked by your
consent settings
Optimization of Salt-Leaching Parameters for Gelatin/Na2Ti3O7 Scaffolds Using a Mixture Design Experiment
(2022-02-01)
Sangkatip, Rittichai
;
Sriseubsai, Wipoo
;
Kiatkittipong, Kunlanan
;
Jongwuttanaruk, Kaona
The purpose of this research was to learn the formation of biomedical scaffold material from gelatin by using titanate (Na<inf>2</inf>Ti<inf>3</inf>O<inf>7</inf>), which is a newly synthesized derivative of titanium dioxide (TiO<inf>2</inf> ) with gelatin. It was prepared by mixed several solutions and cross-linked molecules by heating and salt-leaching. The biomedical scaffold was formed, and its porosity depended on the size of the salt crystal. The mixture was designed by using a mixture design with three factors: gelatin, titanate, and deionized water to determine the optimal mixture for the tensile strength of the biomedical scaffold. The microstructure of the biomedical scaffold was studied using scanning electron microscopy (SEM). The findings revealed that Na<inf>2</inf>Ti<inf>3</inf>O<inf>7</inf> thoroughly pen-extracted the biomedical scaffold, and the tensile strength of the gelatin/titanate scaffold was higher than the biomedical scaffold, which was formed using pure gelatin. By using the mixture design technique, the 14.73% gelatin, 0.2% Na<inf>2</inf>Ti<inf>3</inf>O<inf>7</inf>, and 85.07% DI water got the highest yield of tensile strength (1508.15 kP). This was an about 4.88% increase in the tensile strength property when compared with using TiO<inf>2</inf> .
Some of the metrics are blocked by your
consent settings
Enzymatic degradation of modified gelatin and carboxymethyl cellulose scaffolds
(2018-01-01)
Sutjaritvorakul, Thanawat
;
Wiwatwongwana, Fasai
;
Imsuwan, Pattareewan
;
Whalley, Anthony J.S.
;
Chutipaijit, Sutee
The modification of biological and synthetic biopolymer provides suitable properties for bone and tissue engineering. Modified gelatin and carboxymethyl cellulose (CMC) scaffolds are interesting biomaterials for applying as artificial dermal skin. The objective of this research was to investigate the enzymatic degradation of gelatin and carboxymethyl cellulose scaffolds. The samples were prepared in different compositions of gelatin and CMC (Gelatin:CMC) (w/w) such as 9:1, 8:2, 7:3 and 6:4. The enzymatic degradation test was undertaken using cellulase produced by Aspergillus niger. The results demonstrated that GC64 (Gelatin:CMC, 6:4) exhibited the highest degree of enzymatic degradation and the behavior of the degradation was predicated based on water absorption ability. Moreover, the morphology of degraded residues were investigated by scanning electron microscopy (SEM).
Some of the metrics are blocked by your
consent settings
Physicochemical and functional properties of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as affected by extraction conditions
(2013-01-01)
Kaewruang, Phanngam
;
Benjakul, Soottawat
;
Prodpran, Thummanoon
;
Nalinanon, Sitthipong
Physicochemical and functional properties of gelatin from the skin of unicorn leatherjacket extracted at different temperatures for various times were determined. Yield, recovery and free amino group content of gelatin increased, but gel strength generally decreased as the extraction temperature and time increased (P < 0.05). All gelatins contained a! and a2 chains as the predominant components. FTIR spectra of all gelatins showed a significant loss of the triple-helix. Gels of gelatin extracted at higher temperature for longer time had larger strands with larger voids. At the same level of gelatin, emulsifying and foaming properties varied with extraction conditions. Emulsion activity index (EAI) of all gelatins decreased with increasing concentrations (P < 0.05). Nevertheless, the highest emulsion stability index (ESI) was observed at a level of 3% (P <0.05). Foam expansion (FE) and foam stability (FS) of gelatin generally increased as the concentration increased (P < 0.05). © 2012 Elsevier Ltd.

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results