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Subject: search.filters.subject.Gold nanoparticle

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    A smartphone-integrated rapid and sensitive lateral flow test strip for quantitative detection of Fusarium spp. in maize samples
    (2025-05-01)
    Rotamporn, Saowalak
    ;
    Cheubong, Chehasan
    ;
    Sansenya, Sompong
    ;
    Jantra, Jongjit
    ;
    Teepoo, Siriwan
    In the world, maize is considered as one of the most valuable resources. Fusarium spp. are the primary soilborne plant-pathogenic fungi that can infect maize seeds and cause diseases that potentially decrease the production of crops. This study aims to develop a rapid and sensitive lateral flow test strip (LFTS) utilizing gold nanoparticles (AuNPs) for on-site detection of Fusarium spp. in maize samples. The smartphone-integrated LFTS enables the identification of Fusarium spp. within a concentration range of 0.2–1.5 nM and achieves a limit of detection (LOD) as low as 0.063 nM. The quantitative determination of the Fusarium spp. can be completed within 8 min. Maize samples were analyzed using both the LFTS and real-time polymerase chain reaction (real-time PCR). The LFTS demonstrated effective recovery in spiked samples, with values ranging from 80.1% to 101.1%. Furthermore, the real-time PCR results exhibited significant agreement with the LFTS results. Compared to the traditional real-time PCR technique, the proposed LFTS offers a sensitive, accurate, and rapid assay for the on-site detection of Fusarium spp. in maize samples.
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    Conjugation of antibody on gold nanoparticles for biosensors application
    (2020-01-01)
    Ta-Aithuak, Sarocha
    ;
    Loedsapchinda, Naruenard
    ;
    Houngkamhang, Nongluck
    Regarding to the unique optical property and biocompatible, gold nanoparticles have been widely used to functionalize with antibodies probe for testing specificity with their antigen targets. The antibody immobilized onto gold nanoparticles which synthesized by different methods were studied. Gold nanoparticles synthesized by citrate reduction method and by using poly(ethylene)glycol (PEG) coated gold nanoparticles were immobilized with IgM antibody by physical adsorption. Gold nanoparticles before and after functionalize with antibodies were characterized with fourier-transform infrared spectroscopy (FTIR) for functional group and UV-Vis spectroscopy for absorption wavelength. Finally, a specificity test was conducted using spot of anti-IgM antibody onto nitrocellulose membrane to confirm the bioactivity of antibodies attached to gold nanoparticles.
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    Gold-nanoparticle-based fiber optic sensor for sensing the refractive index of environmental solutions
    (2018-08-01)
    Houngkamhang, Nongluck
    ;
    Charoensuwan, Sittan
    ;
    Sonthipakdee, Onanong
    ;
    Nawattanapaiboon, Kawin
    ;
    Somboonkaew, Armote
    Here an optical fiber modified with gold nanoparticles was successfully fabricated to sense the refractive index of a chemical solution surrounding its surface, with the potential of utilizing this sensor as a biosensing device. Gold nanoparticles, with an average diameter ~20 nm, were synthesized via the citrate reduction method and used to functionalize the glass core of an optical fiber. The sensing principle, which is based on localized surface plasmon resonance, requires the metal gold nanoparticles to be exposed to both the incident and absorbing lights, with the potential detection capabilities determined from the observed light intensity measurement. The optical fiber was uncladded at its center to expose the glass core fiber, and gold nanoparticles were immobilized on the exposed surface using a silane coupling agent. The sensitivity of the gold-nanoparticle-modified optical fibers in measuring the refractive index changes of a solution was compared for unclad lengths ranging between 1 and 2 cm. The attenuation of light depended on both the refractive index of solution and the length of the unclad fiber. The results showed that the fiber sensitivity increased as the unclad length increased due to greater amount of gold on the surface. Antibody-A, which has a specific binding to antigen-A, was then covalently linked to the fiber surface via an amide bond to demonstrate the potential bio-sensing platform of this sensor. The antibody-A functionalized optical fiber was used to detect the red blood cell samples in groups A, B, and O, where it effectively detected both the specific and non-specific binding signals. This fiber optic biosensor therefore provides a low-cost and simple fabrication setup that has potential field applications. Moreover, this setup could potentially be applied to detect other types of whole cell samples.