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Subject: search.filters.subject.Lipid peroxidation

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    Comparison of vapor-phase acetic acid and vinegar effectiveness in maintaining quality of sweet basil (Ocimum basilicum Linn.)
    (2020-01-01)
    Teerarak, Montinee
    ;
    Kerdpiboon, Soraya
    ;
    Krusong, Warawut
    Leafy shoots of sweet basil (Ocimum basilicum Linn.) were exposed to the vapor of either dilute acetic acid (AA) or of upland rice vinegar (URV) – both solutions were diluted to contain 4% of acetic acid – for 10 min and stored at 12°C. The sweet basil exposure to AA had a 16% increase in shelf life and those exposures to URV 35% increase compare to the control. There were no significant differences in fresh weight loss during storage between the AA and URV but both had significantly lower fresh weight losses than the control. The chlorophyll content of both AA and URV were significantly higher than the control. The radical scavenging was significantly higher in the URV than in the AA and the control. The electrolyte leakage was both significantly lower in the URV than in the AA and the control. The occurrence of lipid peroxidation was similar in both the AA and URV, and both were significantly lower than in the control. These results indicate URV has potential as an effective way of raising quality and extending postharvest storage of sweet basil shoots.
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    Essential oil from ginger as a novel agent in delaying senescence of cut fronds of the fern (Davallia solida (G. Forst.) Sw.)
    (2019-10-01)
    Teerarak, Montinee
    ;
    Laosinwattana, Chamroon
    Rabbit's foot fern (Davallia solida (G. Forst.) Sw.) is traded as cut floral greens and commonly used in orchid flower arrangements and bouquets; however, its use is limited by its relatively rapid postharvest yellowing and desiccation. This study was designed to investigate the chemical profile of the essential oil from ginger rhizome (Zingiber officinale Roscoe) and to determine the oil's effectiveness in extending the postharvest quality of rabbit's foot fern. Essential oil was extracted from ginger rhizomes by hydrodistillation. The percentage composition of major identified compounds was citral (29.91%), zingiberene (13.3%), α-farnesene (11.68%), 1,8-cineole (10.60%), and β-sesquiphellandrene (6.14%). The ginger essential oil was applied in emulsion form to rabbit's foot fern fronds as a pulse (25, 50, or 100 mg L<sup>−1</sup>) or a holding solution (5, 10 and 20 mg L<sup>−1</sup>). Pulse treatments were applied for 6 h before the fronds were transferred into a holding solution containing either distilled water or essential oil of ginger. Control fronds received distilled water as both pulsing and holding solutions. Rabbit's foot fern pulsed with 25 mg L<sup>−1</sup> essential oil and transferred into a holding solution with 5 mg L<sup>−1</sup> essential oil increased frond longevity by 4.9 days and helped maintain both water uptake and visual quality. Under this treatment, fronds exhibited higher chlorophyll a and b contents, lower malondialdehyde content, and delayed loss of radical scavenging ability compared to control. Together, these results indicate that when dosed appropriately in pulsing and holding solutions, essential oil of ginger prolongs the vase life of fronds; it also enhances postharvest quality by simultaneously retarding the degradation of chlorophyll a and b, preserving DPPH scavenging activity, and lowering malondialdehyde accumulation.
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    Phytotoxic effects of essential oil from Cymbopogon citratus and its physiological mechanisms on barnyardgrass (Echinochloa crus-galli)
    (2013-01-01)
    Poonpaiboonpipat, Tanatson
    ;
    Pangnakorn, Udomporn
    ;
    Suvunnamek, Umporn
    ;
    Teerarak, Montinee
    ;
    Charoenying, Patchanee
    Lemongrass (Cymbopogon citratus) essential oil was identified by GC-MS for their major compound constituents; six compounds representing 92.59% of the total essential oil were identified. The main constituent was citral (76.00%). In a laboratory bioassay, high concentration of essential oil significantly inhibited germination and seedling growth of Echinochloa crus-galli and affecting α-amylase activity of seeds. In a glass house bioassay, essential oil at concentrations of 1.25, 2.5, 5 and 10% (v/v) was foliar-applied on E. crus-galli at 28 days after sowing at spray volume of 1000Lha <sup>-1</sup>. Observation of leaf wilt symptoms was noted at 6h after treatment. Chlorophyll a, b and carotenoid content was decreased with concentrations of essential oil, indicating that essential oil interferes with photosynthetic metabolism. C. citratus essential oil caused an electrolyte leakage indicating membrane disruption and loss of integrity. Treated leaves exhibited an increase in thiobarbituric acid reactive substances (TBARS), suggesting lipid peroxidation. It is to conclude that essential oil is phytotoxic and could be utilized as a natural herbicide for future weed control. © 2012 Elsevier B.V.
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    High contents of proline and anthocyanin increase protective response to salinity in Oryza sativa L. spp. indica
    (2011-09-01)
    Chutipaijit, Sutee
    ;
    Cha-um, Suriyan
    ;
    Sompornpailin, Kanokporn
    Pigmented rice cultivar is a good candidate for abiotic tolerance especially salt tolerant trait. Physiological and growth parameters of eight indica rice genotypes under control or salt stress conditions were subjected to Hierarchical cluster analysis. From cluster ranking, rice genotypes were classified into two groups, salt-tolerant (Sangyod, Khao Dang, Kulab Dang and TD49) and salt-sensitive genotypes (Klum Sakol, Klum Khonkaen1, Klum Khonkaen2 and Black Sticky Rice). In salt stress (100 mM NaCl), the salt-tolerant genotypes showed the high percentages of proline and anthocyanin more than in the salt-sensitive genotypes. In addition, the photosynthetic pigment stabilizations of stressed-seedlings were positively correlated to the proline (R <sup>2</sup> = 0.98) and anthocyanin (R <sup>2</sup> = 0.70) accumulations. While, the malondialdehyde (MDA) contents of salt-stressed seedlings were negatively correlated to the proline (R <sup>2</sup> = 0.63) and anthocyanin (R <sup>2</sup> = 0.51) accumulations. Moreover, the total antioxidant activity of extraction from stressed-seedlings was assessed using the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) method. The higher percentages of total antioxidant activity were determined in salt-tolerant (125-199%) than salt-sensitive genotypes (106-113%). From the results it can be concluded that accumulation of above mentioned metabolites is associated with cellular protection and salt detoxification in salinity rice seedlings.