Dejtisakdi, Wipawee

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Dejtisakdi, Wipawee
Alternative Name
Dejtisakdi, W.
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Email
wipawee.de@kmitl.ac.th

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    Item type:Publication,
    Optimal conditions for maximized H2 yield from a new green algal strain Chlorella sp. KLSc61
    (2022-08-01)
    Laokua, Natwikar
    ;
    Rittiyan, Nutnicha
    ;
    Kornrawudaphikasama, Yosita
    ;
    Klinsalee, Rachaneekorn
    ;
    Tonawut, Yothawut
    Hydrogen produced from microalgae is attracting the attention of scientists as a potential new ‘Bio-Circular-Green’ energy source. We screened a selection of naturally occurring algal strains for H<inf>2</inf> yield as a basis for high yield development. The best strain was a new strain of biohydrogen-producing green algae from a fresh water source at King Mongkut's Institute of Technology Ladkrabang, Thailand. Using morphological and 18S rDNA sequencing analyses, this alga was identified and classified as Chlorella sp. KLSc61. When adapted cells in TAP-K medium (with starting pH at 9.0) containing 25 mM ethanol, with 54 μmol photons m<sup>−2</sup> s<sup>−1</sup> light, and incubated at 35 °C, Chlorella sp. KLSc61 showed a maximum H<inf>2</inf> yield of 9,804 ± 469.5 μmol H<inf>2</inf> mg<sup>−1</sup> Chl at day 7. Under these conditions, the cells produced ~ 12.2 times more H<inf>2</inf> gas than without optimization (753.0 μmol H<inf>2</inf> mg<sup>−1</sup>Chl). Our optimized system also produced H<inf>2</inf> at a rate 11–570 times greater than previously reported microalgae. Thus Chlorella sp. KLSc61 could be a viable strain for biohydrogen production as a clean future energy source.
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    Proteomic insights into high biohydrogen production by Chlorella sp. KLSc61 under potassium deprivation: upregulation of carbohydrate synthesis proteins
    (2025-12-01)
    Laokua, Natwikar
    ;
    Dejtisakdi, Wipawee
    ;
    Maneeruttanarungroj, Cherdsak
    Microalgae produce hydrogen by utilizing light energy to split water molecules and this produced hydrogen is considered as a promising alternative energy resource. This study investigated the proteomic response of the microalga, Chlorella sp. KLSc61 under an optimal condition for increasing hydrogen production. Chlorella KLSc61 cells were cultured in potassium deprivation medium (TAP-K medium) with an initial pH of 9.0 and added 25 mM ethanol, they were cultivated and exposed to the light intensity of 54 μmol photons m<sup>−2</sup> s<sup>−1</sup> at 35 °C. By day 7, under this growth condition, Chlorella cells produced a maximum H<inf>2</inf> yield of 19,600 ± 0.3 mmol H<inf>2</inf> mg<sup>−1</sup> chlorophyll, which was 2.5 times greater than that under normal TAP condition. Under an increase of hydrogen production, Chlorella KLSc61 cells were both round- and oval-shaped with an average cell diameter of 10.0 ± 0.5 μm, along with variations of chloroplast distribution within the cytoplasm, whereas Chlorella cells in normal TAP medium showed only round shape with an average cell diameter of 5.0 ± 0.5 μm. Proteins from cells grown under normal and high hydrogen conditions were subjected to proteomic analysis, with the results presented as a cluster heat map of proteomics profiles from five different conditions. A total of 736 protein expression patterns were classified into six different expressed protein groups, which included both up- and down-regulated proteins. Carbohydrate synthesis proteins, including starch synthase, glucose-6-phosphate isomerase, glycerol-3-phosphate dehydrogenase, phosphoglucomutase, and UDP-glucuronate decarboxylase, were abundant during the high H<inf>2</inf> production. These findings allow us to take the next step of those protein overexpression into Chlorella KLSc61 cells to enhance in vivo hydrogen production and this strain could be used as a hydrogen production platform.