Poeaim, Supattra

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Poeaim, Supattra
Alternative Name
Poeaim, S.
Poeaim, Supattar
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Email
supattra.poe@kmitl.ac.th

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    Phytochemical screening, antioxidant activity and total phenolic content of methanolic extract of Phak Wan Ton (Crotalaria medicaginea Lam.)
    (2023-01-01)
    Thuion, T.
    ;
    Poeaim, S.
    ;
    Poeaim, A.
    The chlorophyll content of the methanolic extract of Crotalaria medicaginea without chlorophyll removal ranged from 0.50±0.03 to 1.24±0.06 mg/g extract, and with chlorophyll removal ranged from 0.09±0.01 to 0.18±0.00 mg/g extract. The highest total phenolic content using the Folin-Ciocalteu method was presented in the leaves extract without chlorophyll removal as 48.33±0.51 mg GEA/g extract. Moreover, the leaves extract without chlorophyll removal showed the lowest antioxidant activity with 50% inhibitory concentration (IC<inf>50</inf>) in DPPH radical scavenging assay at 344.76 µg/ml. Besides, the root extract exhibited rich potential for gram-positive bacteria, including Bacillus cereus, Bacillus subtilis, Kocuria rhizophila, Staphylococcus aureus and Staphylococcus epidermidis. The overall results showed that the extracts without chlorophyll removal had more efficiency than those with chlorophyll removal. Hence, the phytochemical screening was investigated only in the methanolic extract without chlorophyll removal. All extracts found alkaloids and coumarins, while tannins were found only in roots and did not display saponins. The current study suggested that the extracts of C. medicaginea Lam. gave the potential source of natural bioactive compounds.
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    Bioactive Potential of Gardenia obtusifolia Extracts: Antibacterial Effects of the Ethyl Acetate Fraction against Clinical Pathogens
    (2026-03-01)
    Natsakulmongkon, Phetrung
    ;
    Poeaim, Supattra
    Gardenia obtusifolia, a traditional medicinal plant from Southeast Asia, was investigated for its phytochemical composition and bioactivity using methanol extracts and solvent-partitioned fractions from the leaves, fruit pulp, and rind. The plant material was authenticated morphologically and confirmed through matK gene analysis. Phytochemical screening revealed the presence of phenolic compounds, flavonoids, tannins, coumarins, and alkaloids. Among the fractions tested, the rind ethyl acetate fraction exhibited strong antibacterial activity against clinical pathogens, high phenolic content, and potent antioxidant capacity. GC-MS profiling identified key bioactive compounds, including vanillic acid, octadecane, and 5-hydroxymethylfurfural, which likely contribute to the observed bioactivities. Among the solvent fractions, the rind ethyl acetate fraction demonstrated strong anti-tyrosinase activity. This fraction consistently exhibited high phenolic content and potent antioxidant capacity, suggesting that these bioactivities are attributable to key phytochemicals known for antioxidant effects. Purification and GC-MS profiling identified bioactive compounds in the rind ethyl acetate fraction, revealing vanillic acid as a major contributor to antioxidant activity. G. obtusifolia shows great promise for application in the cosmetic industry as a natural ingredient for skin-whitening and anti-aging formulations. However, further in vivo and clinical studies are required, as in vitro results may not directly reflect clinical efficacy.