Somala, Naphat

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Somala, Naphat
Alternative Name
Somala, N.
Main Affiliation
Email
naphat.so@kmitl.ac.th

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    Item type:Publication,
    Ultrasonic emulsification of Cananga odorata nanoemulsion formulation for enhancement of herbicidal potential
    (2025-12-01)
    Laosinwattana, Chamroon
    ;
    Somala, Naphat
    ;
    Dimak, Jantra
    ;
    Teerarak, Montinee
    ;
    Chotsaeng, Nawasit
    A nanoemulsion was fabricated from Cananga odorata essential oil (EO) and stabilized by incorporation of Tween 80 using ultrasonication. The major constituents of the EO were benzyl benzoate, linalool, and phenylmethyl ester. Differing sonication amplitude (20–60%) and time (2–10 min) were assessed for effects on nanoemulsion droplet size and polydispersity index (PI). The smallest droplet size of 43.98 nm (PI 0.222) was obtained using 40% amplitude for 8 min; this nanoemulsion was evaluated for its droplet characteristics and pre-emergence herbicidal activities on Amaranthus tricolor. FT-IR confirmed ultrasonic emulsification to not affect the EO components. Regarding stability, storage at 4 °C was determined appropriate, with droplet size changing slightly after five weeks. Assays of herbicidal potential showed the coarse emulsion and nanoemulsion to both reduce A. tricolor germination and growth, with the nanoemulsion being more effective at a given concentration and the difference in effectivity correlating to droplet size. Remarkably, treatment with 250 ppm nanoemulsion and coarse emulsion respectively resulted in 100% and 63.75% germination inhibition. Both emulsions decreased seed imbibition and alpha-amylase activity. The highest relative electrolyte leakage was achieved in seed treated with the nanoemulsion. Therefore, this ultrasonic-based nanoemulsion may have utility as bioherbicide alternative.
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    Allelopathic potential of secondary metabolites produced by Alternaria brassicicola and physiological mechanisms on Amaranthus tricolor
    (2023-05-01)
    Manichart, N.
    ;
    Somala, N.
    ;
    Laosinwattana, C.
    The results revealed that all isolated fungal strains significantly reduced germination and early growth of amaranth compared to control. The original metabolites (1.00X) from the fungus coded UNK03, which was identified as a strain of Alternaria brassicicola (98.44% similarity, accession number LC440588.1), showed the highest inhibition rate and suppressed with the maximum inhibitory level (78.75%) compared to control. In a similar pattern, the growth of the tested plant was significantly reduced by the cultural filtrates by 70.45% and 89.38% in shoot and root, respectively. Based on efficacy in the seed bioassay, the A. brassicicola metabolites (CF03) were selected for exposure with the tested seed to determine the mechanism of action (MOA). The results show that the A. brassicicola metabolites inhibited seed imbibition and -amylase activity of the tested seed with dose-dependent effects, strongly suggesting the potential of the metabolites to inhibit seed germination and seedling growth. Therefore, this work indicates that the fungal metabolites contain potent herbicidal compounds that can potentially be used to control A. tricolor.
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