Pornpukdeewattana, Soisuda

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Preferred name
Pornpukdeewattana, Soisuda
Alternative Name
Pornpukdeewattana, S.
Main Affiliation
Email
soisuda.po@kmitl.ac.th

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    Item type:Publication,
    Development of a synbiotic longan beverage: a nutritious and functional blend
    (2025-02-01)
    Sakuntasri, K.
    ;
    Kwanmuang, P.
    ;
    Massa, S.
    ;
    Krusong, W.
    ;
    Charoenrat, T.
    Synbiotic foods, which synergistically combine probiotics and prebiotics, have garnered attention for their potential health benefits. In line with this trend, this study aimed to develop a synbiotic beverage using longan fruit as the base and fructo-oligosaccharides (FOS) as the prebiotic source. Two strains of Lactobacillus probiotics, Lactobacillus casei 431 and Lactobacillus plantarum KM001, were used to ferment the longan juice with and without FOS. Total soluble solids, reducing sugar, pH, total acid production, and viable cell count of Lactobacillus microorganisms were evaluated to determine the optimal fermentation duration. The findings indicated that fermenting the juice for 12 hrs at 37°C aligned with the criteria established by the FAO/WHO for probiotic foods. Furthermore, compared to L. casei 431, the longan juice containing FOS fermented with L. plantarum KM001 showed a better survival rate in a simulated gastrointestinal environment. Additionally, the microorganism count in this beverage remained relatively constant throughout its 28-days shelf life at 4°C. These findings underscore the synbiotic longan beverage as a functional drink that combines viable probiotics with prebiotic FOS.
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    Item type:Publication,
    High-cell-density culture for recombinant xylanase production and its application in hydrolysis of mild alkaline pretreated rice straw for xylooligosaccharide production
    (2026-08-01)
    Laemthong, Tunyaboon
    ;
    Sukhumsirichart, Wasana
    ;
    Chittapun, Supenya
    ;
    Kongsinkaew, Chatchol
    ;
    Satipattarn, Atikant
    Rice straw is an abundant lignocellulosic residue whose effective utilization requires coordinated enzyme performance and pretreatment conditions. In this study, recombinant xylanase production was integrated with mild alkaline pretreatment to enable efficient conversion of rice straw into xylooligosaccharides (XOS). Recombinant xylanase was produced by Escherichia coli using high-cell-density fed-batch cultivation, achieving a maximum activity of 207.95 U/mL in an enriched synthetic medium. Alkaline pretreatment was optimized using response surface methodology, identifying 6.75% (w/v) biomass loading, 193.17 mM NaOH, and 20 min as conditions that balanced solid recovery with sugar release during subsequent enzymatic hydrolysis. Hydrolysis of the pretreated rice straw yielded 6.13 mg/mL total XOS, mainly xylotriose (X3, 2.73 mg/mL; 44.56%), xylobiose (X2, 2.00 mg/mL; 32.62%), and xylotetraose (X4, 1.02 mg/mL; 16.57%), with only minor amounts of xylose and xylopentaose detected. The combined X2 and X3 fractions accounted for 77.18% of the total XOS. XOS with degrees of polymerization of 2–4 are recognized as effective prebiotics, particularly for beneficial intestinal bacteria such as Bifidobacterium spp. Overall, linking recombinant enzyme production with alkaline pretreatment improved conversion consistency and supported efficient XOS production from rice straw.