Sontigun, Narin

Loading...
Thumbnail Image
Preferred name
Sontigun, Narin
Main Affiliation
Email
narin.so@kmitl.ac.th

Settings

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Preliminary study on prevalence of hemoprotozoan parasites harbored by Stomoxys (Diptera: Muscidae) and tabanid flies (Diptera: Tabanidae) in horse farms in Nakhon Si Thammarat province, Southern Thailand
    (2023-01-01)
    Phetkarl, Tanakorn
    ;
    Fungwithaya, Punpichaya
    ;
    Udompornprasith, Supak
    ;
    Amendt, Jens
    ;
    Sontigun, Narin
    Background and Aim: Stomoxys and tabanid flies are of medical and veterinary importance because they play crucial roles in disease transmission as mechanical vectors of various hemopathogens. However, its role as a hemoprotozoan parasite vector in horse farms has not been studied. Therefore, we investigated the occurrence of hemoprotozoan parasites belonging to the genera Babesia, Theileria, and Trypanosoma in Stomoxys and tabanid flies using conventional polymerase chain reaction (PCR) and DNA sequencing. Materials and Methods: All samples (Stomoxys and tabanid flies) were collected using an Nzi trap for three consecutive days each month from November 2022 to March 2023. The flies were morphologically identified to the species level and separated according to sex. Individual (for tabanid flies) or pooled samples (consisting of three specimens of Stomoxys flies of the same species and sex collected from the same site) were used for DNA extraction. Conventional PCR was used to screen for hemoprotozoan parasite DNA, followed by Sanger sequencing to identify the species. Results: In total, 189 biting flies were collected, including four species of Stomoxys (Stomoxys bengalensis, Stomoxys calcitrans, Stomoxys indicus, and Stomoxys sitiens) and five species of tabanids (Atylotus cryptotaxis, Chrysops dispar, Tabanus megalops, Tabanus mesogaeus, and Tabanus rubidus). Stomoxys calcitrans was the most prevalent species, accounting for 58.7% (n = 111) of the collected flies. Ten (12.4%) of the 81 samples (individuals and pools) analyzed by PCR were positive for the 18S rRNA gene of the Theileria/Babesia species. Trypanosoma DNA was not detected in any sample. After performing Basic Local Alignment Search Tool searches and a phylogenetic analysis, only six samples (7.4%), including S. calcitrans (n = 2), S. sitiens (n = 2), T. megalops (n = 1), and A. cryptotaxis (n = 1), were found to be infected with Theileria sinensis. Furthermore, apicomplexan parasites, namely, Mattesia spp. and Colpodella spp., were found on S. indicus, the fungus Meira spp. was found on S. calcitrans, and the pathogenic green alga Helicosporidium spp. was found on A. cryptotaxis. Conclusion: This study is the first to report a variety of Stomoxys and tabanid flies collected from horse farms in Thailand, which were found to be infected with Theileria and Colpodella species that affect mammals, suggesting that Stomoxys and tabanid flies can be used to confirm the presence of hemoprotozoan parasites in the study area. Understanding the presence of hemoprotozoa in flies could help design vector control programs and manage various diseases in the study area.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Species Diversity and Seasonal Abundance of Stomoxyinae (Diptera: Muscidae) and Tabanid Flies (Diptera: Tabanidae) on a Beef Cattle and a Buffalo Farm in Nakhon Si Thammarat Province, Southern Thailand
    (2024-10-01)
    Phetcharat, Yotsapat
    ;
    Wongtawan, Tuempong
    ;
    Fungwithaya, Punpichaya
    ;
    Amendt, Jens
    ;
    Sontigun, Narin
    This study investigated species diversity and seasonal abundance of Stomoxyinae and tabanid flies, which are significant pests and vectors of animal pathogens, on a beef cattle and a buffalo farm in Nakhon Si Thammarat province, southern Thailand. During a one-year period from December 2020 to November 2021, flies were collected using Nzi traps from 6 a.m. to 6 p.m. over three consecutive days each month, resulting in the capture of 1912 biting flies, representing seven Stomoxyinae and nine tabanid species. The five most prevalent species were Tabanus megalops, Haematobia irritans exigua, Stomoxys calcitrans, Stomoxys indicus, and Stomoxys uruma. Fly density was notably higher on the beef cattle farm compared to the buffalo farm, with most species peaking during the rainy season, except for H. i. exigua, which was more abundant during the dry season. This study also examined the influence of temperature, relative humidity, and rainfall on fly density, revealing species-specific patterns. These findings offer updated insights into species diversity and seasonal trends, providing critical baseline data essential for the development of effective control strategies aimed at mitigating the impact of these flies on livestock health.