Chysirichote, Teerin

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Chysirichote, Teerin
Alternative Name
Chysirichote, T.
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Email
teerin.ch@kmitl.ac.th

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    Item type:Publication,
    Comparative Evaluation of Culture Media and pH Conditions for Xylanase Production in Aspergillus niger
    (2025-09-01)
    Pakaweerachat, Pattarabhorn
    ;
    Chysirichote, Teerin
    Xylanase plays a crucial role in the degradation of hemicellulose and holds significant promise across a variety of biotechnological industries. This study investigates the effects of culture medium composition and initial pH on xylanase production by Aspergillus niger. Five different media; Mandels’, Vogel’s, Marciel’s, Okafor’s, and a modified Czapek-Dox, were evaluated for their ability to support enzyme synthesis. Additionally, initial pH levels ranging from 4.0 to 8.0 were tested to identify optimal production conditions. Among the tested media, Mandels’ medium supported the highest xylanase activity, while the optimal pH range for enzyme production was between 6.0 and 7.0. These findings provide valuable insights for optimizing large-scale xylanase production using A. niger, contributing to the development of more efficient and cost-effective bioprocesses. Notably, this study incorporates biomass-specific productivity metrics (IU/g biomass), allowing for a more accurate evaluation of the strain’s enzymatic efficiency. This quantitative approach may help inform future efforts to develop cost-effective and scalable bioprocesses, particularly when applied to low-cost agro-industrial substrates.
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    Dielectric constant-based optimization for sustainable extraction of centellosides from Centella asiatica using bio-based solvents
    (2026-08-01)
    Pakaweerachat, Pattarabhorn
    ;
    Chysirichote, Teerin
    This study systematically examined the influence of solvent polarity and extraction temperature on the recovery of four major centellosides: madecassoside (MAD), asiaticoside (ASI), madecassic acid (MDA), and asiatic acid (ASA) from Centella asiatica. Extractions were performed using ethanol-water, acetone-water, ethanol-ethyl acetate, and hexane-ethyl acetate mixtures at 30–70 °C, covering dielectric constants (ε = 2–70) that correspond to the polarity range of the selected solvent systems. Extraction yields (mg g⁻¹) are expressed on a dry-weight basis. Ethanol–water mixtures demonstrated the highest efficiency for polar glycosides, yielding 19.6 ± 0.3 mg g⁻¹ dry matter (mg g⁻¹ DM) for MAD and 17.2 ± 0.3 mg g⁻¹ DM for ASI at 70 °C, with temperature effects mainly attributed to enhanced mass transfer and no significant thermal degradation observed. In contrast, aglycones (MDA and ASA) achieved optimal yields (3.3–6.5 mg g⁻¹) in solvents with lower dielectric constants, where solvent composition predominated over temperature effects. Solvent polarity, expressed in terms of dielectric constant, provided a quantitative indicator linking solvent selection to centelloside recovery and solvent-temperature interactions. Ethanol-water systems are recommended for food and nutraceutical applications, while ethyl acetate-ethanol mixtures are suited for cosmetic formulations. This work establishes a polarity-temperature framework for enhancing extraction using bio-based solvents, contributing to more sustainable production of bioactive-rich extracts from C. asiatica for use in food, cosmetic, and pharmaceutical industries.