Sanguanchaipaiwong, Vorapat
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Preferred name
Sanguanchaipaiwong, Vorapat
Alternative Name
Sanguanchaipaiwong, V.
Main Affiliation
Email
vorapat.sa@kmitl.ac.th
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Item type:Publication, Using Glycerol as a Sole Carbon Source for Clostridium beijerinckii Fermentation(2017-01-01); Leksawasdi, NoppolButanol is a promising biofuel with its fuel properties which are similar to gasoline and able to be synthesized from acetone-butanol-ethanol fermentation of Clostridium sp. This study has been focused on using glycerol, a by-product from biodiesel manufacturing as a carbon source for Clostridium beijerinckii TISTR 1390. The culture was cultivated at 37°C in P2 medium with 20-60 g/L glycerol under anaerobic condition, compared with glucose as a control. Samples were collected periodically for the analysis of viable cell concentration, reducing sugar and metabolite concentrations. It has been found that C. beijerinckii grown much slower in glycerol. After 168 h cultivation, the cell growth in 20 g/L glycerol has achieved the maximum concentration of 4.78 × 10<sup>6</sup> CFU/mL. In P2 medium containing glucose, the amount of viable cell (3.93 x 10<sup>6</sup> CFU/mL with 20 g/L glucose) had reached the maximum level at 24 h. The much longer period for highest cell number might be the reason that C. beijerinckii could not produce butanol from merely glycerol. On the other hand, the butanol concentration of 8.12 g/L was obtained from 60 g/L glucose. Glycerol probably induced C. beijerinckii in switching to a reductive pathway. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Butanol production by Clostridium beijerinckii from pineapple waste juice(2018-01-01); Leksawasdi, NoppolRenewable energy has received increasing attention, due to global energy crisis and limited supply of fossil fuels. Butanol is one of the alternative biofuels with the similar energy properties as gasoline, such as, energy density and heat of vaporization. It could be produced by Clostridia via acetone-butanol-ethanol fermentation from various renewable sources. To obtain economical raw materials, pineapple waste juice was utilized as a carbon source for Clostridium beijerinckii TISTR 1461. The juice was collected from ‘Pattavia' pineapple waste and utilized to prepare culture medium. The maximum viable C. beijerinckii concentration (2.40 ± 0.12 x 10<sup>8</sup> CFU/mL) was obtained at 168 h of cultivation with pineapple waste juice under anaerobic condition at 37 °C. The butanol concentration of 3.14 ± 0.16 g/L was subsequently produced. A yield of 0.08 g butanol∙g<sup>-1</sup> reducing sugars was achieved suggesting the necessity to improve fermentation process for higher level of butanol concentration.