Sanguanchaipaiwong, Vorapat

Loading...
Thumbnail Image
Preferred name
Sanguanchaipaiwong, Vorapat
Alternative Name
Sanguanchaipaiwong, V.
Main Affiliation
Email
vorapat.sa@kmitl.ac.th

Settings

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Butanol production by Clostridium beijerinckii from pineapple waste juice
    (2018-01-01)
    Sanguanchaipaiwong, Vorapat
    ;
    Leksawasdi, Noppol
    Renewable energy has received increasing attention, due to global energy crisis and limited supply of fossil fuels. Butanol is one of the alternative biofuels with the similar energy properties as gasoline, such as, energy density and heat of vaporization. It could be produced by Clostridia via acetone-butanol-ethanol fermentation from various renewable sources. To obtain economical raw materials, pineapple waste juice was utilized as a carbon source for Clostridium beijerinckii TISTR 1461. The juice was collected from ‘Pattavia' pineapple waste and utilized to prepare culture medium. The maximum viable C. beijerinckii concentration (2.40 ± 0.12 x 10<sup>8</sup> CFU/mL) was obtained at 168 h of cultivation with pineapple waste juice under anaerobic condition at 37 °C. The butanol concentration of 3.14 ± 0.16 g/L was subsequently produced. A yield of 0.08 g butanol∙g<sup>-1</sup> reducing sugars was achieved suggesting the necessity to improve fermentation process for higher level of butanol concentration.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    PRETREATMENT AND HYDROLYSIS OF EUCALYPTUS FOR BIOBUTANOL PRODUCTION USING CLOSTRIDIUM SP. G10
    (2025-01-01)
    Rinthanapipat, Tunlaya
    ;
    Wongyou, Tarinee
    ;
    Sanguanchaipaiwong, Vorapat
    The primary objective of this study was to optimize the pretreatment and hydrolysis of eucalyptus for the cultivation of Clostridium sp. G10, a laboratory-isolated strain. Optimal alkali pretreatment conditions were determined to be 0.6 M NaOH at 121°C and 15 psi for 20 minutes, yielding a reducing sugar concentration of 11.91 g/L. Subsequent cellulose hydrolysis using ACCELERASE® 1500 (2000 endoglucanase U/g eucalyptus) for 24 hours further increased the reducing sugar concentration to 15.53 g/L. Eucalyptus hydrolysate was then utilized as a carbon source for Clostridium sp. G10 cultivation. In T6 medium supplemented with 50 g/L glucose, the maximum concentration of acetone-butanol-ethanol was 2.7 g/L. When T6 medium was supplemented with eucalyptus hydrolysate (50 g/L reducing sugar equivalent), the solvent concentration was found at 1.21 g/L. The results clearly indicate that the type of medium significantly impacts the production of acetone, butanol, and ethanol by Clostridium sp. G10. While eucalyptus hydrolysate can serve as a suitable carbon source, further optimization of hydrolysis conditions is necessary to achieve higher reducing sugar concentrations. Additionally, the removal of fermentation inhibitors from the hydrolysate is crucial for enhancing the efficiency of acetone-butanol-ethanol fermentation.