Wongkaewkhiaw, Saharut

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Wongkaewkhiaw, Saharut
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Email
saharut.wo@kmitl.ac.th

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    Item type:Publication,
    Oil-in-water nanoemulsion-based ethyl lauroyl arginate nanoparticles exhibit potent antibacterial and antibiofilm activity against oral pathogens
    (2026-04-23)
    Wongkaewkhiaw, Saharut
    ;
    Pairojana, Tanita
    ;
    Chansiri, Sirirat
    ;
    Tsao, Stephany
    ;
    Panmekiate, Soontra
    Background. Dental biofilm is a key etiological factor in oral diseases such as dental caries and periodontal disease, and has also been linked to systemic health complications. In this study, ethyl lauroyl arginate nanoparticles (ELANPs) were formulated using an oil-in-water nanoemulsion system to investigate their antibiofilm capacity and cellular cytotoxicity in vitro. Methods. The morphology of ELANPs was examined using transmission electron microscopy (TEM). Particle size, polydispersity index (PDI), and zeta potential were evaluated to assess nanoparticle stability over time. Biofilm matrix reduction was analyzed using confocal laser scanning microscopy (CLSM), and cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results. ELANPs displayed a spherical morphology with an average diameter of 84.3 ± 2.6 nm. The zeta potential was 46.7 ± 4.6 mV, and the PDI was 0.18 ± 0.01, indicating good colloidal uniformity and stable nanosuspensions. Slight changes in physicochemical properties were observed at days 14 and 30, and overall stability was maintained. ELANPs significantly reduced the biofilm matrix across all tested oral pathogens while maintaining low cytotoxicity toward normal human gingival fibroblasts. Conclusion. The findings indicate that ELANPs are physicochemically stable, capable of inhibiting oral biofilm formation, and exhibit minimal cytotoxicity, supporting their potential as a safe and effective oral healthcare agent.
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    Cholesterol-lowering effects of lactic acid bacteria isolated from Musa sapientum Linn.
    (2022-09-01)
    Rattanachak, Nontaporn
    ;
    Nualsri, Chatchawin
    ;
    Nittayasut, Naiyaphat
    ;
    Srisayam, Montra
    ;
    Chodnakarin, Arunluk
    Hypercholesterolemia is generally recognised as a risk factor for cardiovascular disease. Musa sapientum Linn. or Kluai Namwa (banana; ABB genome type) provides a functional nutrient in health promotion products and is a source of various probiotics. Lactic acid bacteria (LAB) isolated from several sources are reported with cholesterol-lowering potential. However, the effects of LAB isolated from bananas on total cholesterol are still unknown. The aim of this study is to investigate the cholesterol-removing ability of 5 LAB strains isolated from raw M. sapientum. All tested strains show a cholesterol-lowering property in species-and strain-dependent manner, with Weissella paramesenteroides NR27 being the most effective probiotic in reducing cholesterol concentration (P < 0.001). The removal of cholesterol by LAB is partially due to the production of bile salt hydrolase. All tested isolates exhibit more than 75% and 50% survival rates after challenging with simulated gastric and intestinal fluids respectively. Of these, W. paramesenteroides NR27 has a significantly higher survival rate (80%) in gastrointestinal conditions (P < 0.001), compared with all tested strains. In addition, antibiotic-resistant genes are not present in all drugs tested on this isolate. Therefore, W. paramesenteroides NR27 could be a promising probiotic candidate for reducing total cholesterol.