Kingkaew, Engkarat
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Item type:Publication, Bioethanol production from cassava starch using co-culture of saccharolytic molds with Saccharomyces cerevisiae TISTR 5088(2024-08-01); Cassava serves as a cornerstone for sustainable bioethanol production in Thailand. The cassava root is highly valuable, comprising 50–70% starch on a dry weight basis. Starch can be converted into fermentable sugars through chemical or biological methods. This study focused on harnessing the enzymatic potential of saccharolytic molds, specifically Aspergillus oryzae TISTR 3086 or Amylomyces rouxii TISTR 3182, to facilitate saccharification of cassava starch, followed by fermentation using Saccharomyces cerevisiae TISTR 5088 for bioethanol production. Among the various configurations, the co-culture of Amy. rouxii TISTR 3182 with S. cerevisiae TISTR 5088 was the most effective, resulting in the highest ethanol yield. This finding was observed for both the separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes. Impressively, the SSF approach to ethanol productivity demonstrated superior results with an ethanol concentration of 25.4±0.3 g/l and an ethanol productivity of 0.53 g/l/h (yield of 1.40 g/g reducing sugars), surpassing the SHF approach (25.4±0.7 g/l, an ethanol productivity of 0.26 g/l/h, yield of 0.57 g/g reducing sugars) while also reducing the fermentation period. Further investigations into optimizing the conditions for ethanol production were carried out using a co-culture of Amy. rouxii TISTR 3182 with S. cerevisiae TISTR 5088 during SSF. This exploration revealed that employing 100 g/l cassava starch and initiating fermentation with a medium pH of 6.0 led to the highest ethanol concentration at 48 h. This process showed potential for ethanol production, harnessing the synergistic action of saccharolytic molds alongside yeast. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antioxidant Activity, γ-Aminobutyric Acid, and Genome Analysis of Lactiplantibacillus plantarum FL13-2 from Fermented Rice Flour(2025-09-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Evaluating the safety and efficacy of Lacticaseibacillus paracasei TISTR 2593 as a therapeutic probiotic for obesity prevention(2025-01-01) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Anaerobic flora, Selenomonas ruminis sp. nov., and the bacteriocinogenic Ligilactobacillus salivarius strain MP3 from crossbred-lactating goats(2024-12-01); Nuengjamnong, ChackritThis study aimed to examine the distribution of anaerobic bacteria in the rumen fluid of Thai crossbred goats and to screen potential probiotic strains capable of producing antimicrobial compounds and inhibiting bacteria that cause milk fat depression. Thirty-four strains of bacteria from the rumen fluid were divided into 13 groups within 12 genera based on 16S rRNA gene sequences. The RF1-5 and RF5-12 were identified as Streptococcus luteliensis and Bacillus licheniformis, respectively, and demonstrated non-ropy exopolysaccharide. Furthermore, mPRGC5<sup>T</sup> was closely related to Selenomonas caprae JCM 33725<sup> T</sup> (97.8% similarity) based on 16S rRNA gene sequences. It exhibited low average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity values with related type strains ranging from 84.9 to 86.0%, 21.3 to 21.8%, and 73.8 to 76.1%, respectively. The genotypic and phenotypic characteristics of mPRGC5<sup>T</sup> strongly support this strain as a new species of the genus Selenomonas for which the name Selenomonas ruminis mPRGC5<sup>T</sup> was proposed. The type strain is mPRGC5<sup>T</sup> (= JCM 33724<sup> T</sup> = KCTC 25177<sup> T</sup>). Ligilactobacillus salivarius MP3 showed antibacterial activity against Cutibacterium acnes subsp. acnes DSM 1897<sup> T</sup> and Kocuria rhizophila MIII. The enterolysin A cluster gene was identified in its genome. The auto-aggregation of L. salivarius MP3 was 93.6 ± 0.2%. Additionally, co-aggregation of L. salivarius MP3 with C. acnes DSM 1897<sup> T</sup> and K. rhizophila MIII had 92.2 ± 3.4% and 87.3 ± 4.5%, respectively. The adhesion capacity of strain MP3 was 76.11 ± 2.2%. Probiogenomic analysis revealed that L. salivarius MP3 was nonhazardous to animal supplementation and included acid- and bile-tolerant ability. However, strain MP3 contained three antibiotic resistance genes. Thus, the supplementation of L. salivarius MP3 could increase the milk fat content by suppressing C. acnes DSM 1897<sup> T</sup> with antibiotic resistance gene horizontal transfer awareness. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Integrative genome analysis of bacteriocin-producing Lactiplantibacillus pentosus LNP1-39 and its synbiotic role in suppressing food-borne pathogens(2026-06-01); Poothong, SaranpornLactic acid bacteria were isolated from traditional Thai-fermented foods. Among these, the strain LNP1-39, closely related to Lactiplantibacillus pentosus, was selected for further study because of its non-pathogenic profile. The bacteriocins produced by L. pentosus LNP1-39 were proteinaceous substances that exhibited strong antimicrobial activity across a wide pH range (pH 2–11; 6400–2400 AU/mL) and thermal stability at 100 °C for 40 min (400 AU/mL). These bacteriocins showed a narrow antimicrobial spectrum, effectively targeting Gram-positive pathogens, such as Kocuria rhizophila MIII, Enterococcus faecalis JCM 5803<sup> T</sup>, and Listeria monocytogenes ATCC 19115. Comprehensive safety assessments, including whole-genome analysis and in vitro tests, confirmed a low risk of antibiotic resistance and the absence of virulence factors. Strain LNP1-39 was confirmed to be closely related to L. pentosus DSM 20314<sup> T</sup> via digital DNA‒DNA hybridization (dDDH; 75.4%), with average nucleotide identity (ANI) at 96.56% ANIb and 97.22% ANIm values. Additionally, LNP1-39 produces pediocin with notable similarity (76.29% identity to pediocin) and presents low risks for antibiotic-resistance genes or transfer genes while providing antioxidant properties. Strain LNP1-39 survived harsh gastrointestinal tract conditions and exhibited a favorable prebiotic index and positive prebiotic activity score when paired with polydextrose or isomalto-oligosaccharide. These findings support L. pentosus LNP1-39 as potential bacteriocin-producing lactic acid bacteria for further application in food preservation and pathogen control or as a synbiotic. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Genomic insights into arsenic and antibiotic resistance in Comamonas thiooxydans strains F1-6 and A7-5(2026-04-01); Lee, Jung SookTwo arsenic-resistant, Gram-negative, aerobic rod-shaped bacterial strains, F1-6 and A7-5, which were isolated from agricultural soil in Thailand, were systematically studied. They grew at 30 °C, pH 5–11, and in 3% (w/v) NaCl. Ubiquinone with eight isoprene units (Q-8) and the cellular fatty acids C1<inf>6:0</inf>, C<inf>18:1</inf> ω7c, and C<inf>17:0</inf> CYCLO were the major components. Phylogenetic analysis via 16S rRNA gene sequences revealed that strains F1-6 and A7-5 were affiliated with the genus Comamonas and closely related to C. thiooxydans DF2<sup>T</sup> and C. testosteroni KCTC 2990<sup>T</sup>, with 99.79% and 99.86% similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between F1-6 and C. thiooxydans DF2<sup>T</sup> were 97.89% and 85.7%, respectively, whereas those between A7-5 and C. thiooxydans DF2<sup>T</sup> were 97.23% and 81.3%, respectively. Thus, both the F1-6 and A7-5 were identified as C. thiooxydans. The draft genome sizes of F1-6 and A7-5 were 5.2 and 5.3 Mb, comprising 87 and 84 contigs, with DNA G+C contents of 61.5% and 61.4%, respectively. The genomes of both strains contained ars cluster genes and many genes for growth and resistance to heavy metals and antibiotics, similar to those of C. testosteroni ATCC 11996<sup>T</sup>, C. thiooxydans CNB-1 substr. CNB-2 Chr, and C. terrae NBRC 106524<sup>T</sup>. Nevertheless, the acr3, qacG, and vanH genes in the vanO cluster were found only in C. terrae NBRC 106524<sup>T</sup>. This study provides more comprehensive insight into As-resistant bacteria and could be applied to the bioremediation of As and other heavy metals in the future. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Lentilactobacillus terminaliae sp. nov., isolated from tree bark (Terminalia ivorensis Chev.) and its antioxidant activity(2025-01-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Diversity, astaxanthin production, and genomic analysis of Rhodotorula paludigena SP9-15(2023-07-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Genomic Assessment of Potential Probiotic Lactiplantibacillus plantarum CRM56-2 Isolated from Fermented Tea Leaves(2024-07-31); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Optimization of astaxanthin production by Rhodotorula toruloides CB6-10/1 using response surface methodology and its genome analysis(2026-02-01);