Vatanavicharn, Tipachai

Loading...
Thumbnail Image
Preferred name
Vatanavicharn, Tipachai
Main Affiliation
Email
tipachai.va@kmitl.ac.th

Settings

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Role of cyclophilin A as an immunostimulator in responses to Vibrio parahaemolyticus AHPND infection in the black tiger shrimp Penaeus monodon
    (2025-11-01)
    Towittayapanchai, Pornsawan
    ;
    Ponprateep, Sirikwan
    ;
    Supungul, Premruethai
    ;
    Tang, Sureerat
    ;
    Jaree, Phattarunda
    Cyclophilin A (CypA) is a highly conserved peptidyl-prolyl isomerase that plays a crucial role in protein folding, immune regulation, and cellular stress responses. In shrimp, CypA has been implicated in innate immunity, particularly in response to viral and bacterial infections. This study investigates the role of the Penaeus monodon cyclophilin A (PmCypA) in shrimp infected with V. parahaemolyticus AHPND (VP<inf>AHPND</inf>). During VP<inf>AHPND</inf> infection, the expression levels of PmCypA gene and PmCypA protein were significantly increased at 24 h post-infection (h.p.i.) in shrimp hemocyte. To investigate the role of PmCypA in shrimp immune response, shrimp were administrated with the recombinant PmCypA protein (rPmCypA) and submerged in VP<inf>AHPND</inf> suspension. The results showed that the rPmCypA could rapidly stimulate the expression of immune-related genes involved in several immune pathways, such as immune recognition receptors, clotting system, phagocytosis and homeostasis. Moreover, the injected rPmCypA led to an increase in the proportion of hyaline cells and the greater quantity of granulocytes that contained PmCypA. Furthermore, the rPmCypA could prolong shrimp survival during VP<inf>AHPND</inf> infection. These results suggest that the PmCypA is a bacterial-responsive gene in shrimp immune defense response and potentially an immunostimulator in shrimp.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    PmVRP15, a novel viral responsive protein from the black tiger shrimp, Penaeus monodon, promoted white spot syndrome virus replication
    (2014-03-17)
    Vatanavicharn, Tipachai
    ;
    Prapavorarat, Adisak
    ;
    Jaree, Phattarunda
    ;
    Somboonwiwat, Kunlaya
    ;
    Tassanakajon, Anchalee
    Suppression subtractive hybridization of Penaeus monodon hemocytes challenged with white spot syndrome virus (WSSV) has identified the viral responsive gene, PmVRP15, as the highest up-regulated gene ever reported in shrimps. Expression analysis by quantitative real time RT-PCR revealed 9410-fold up-regulated level at 48 h post WSSV injection. Tissue distribution analysis showed that PmVRP15 transcript was mainly expressed in the hemocytes of shrimp. The full-length cDNA of PmVRP15 transcript was obtained and showed no significant similarity to any known gene in the GenBank database. The predicted open reading frame of PmVRP15 encodes for a deduced 137 amino acid protein containing a putative transmembrane helix. Immunofluorescent localization of the PmVRP15 protein revealed it accumulated around the nuclear membrane in all three types of shrimp hemocytes and that the protein was highly up-regulated in WSSV-infected shrimps. Double-stranded RNA interference-mediated gene silencing of PmVRP15 in P. monodon significantly decreased WSSV propagation compared to the control shrimps (injected with GFP dsRNA). The significant decrease in cumulative mortality rate of WSSV-infected shrimp following PmVRP15 knockdown was observed. These results suggest that PmVRP15 is likely to be a nuclear membrane protein and that it acts as a part of WSSV propagation pathway. Copyright: © 2014 Vatanavicharn et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.