Vatanavicharn, Tipachai

Preferred name

Main Affiliation

School of Science

tipachai.va@kmitl.ac.th

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Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai
(2017-03-01)
Ponprateep, Sirikwan
;
Vatanavicharn, Tipachai
;
Lo, Chu Fang
;
Tassanakajon, Anchalee
;
Rimphanitchayakit, Vichien
The shrimp multifunctional protein alpha-2-macroglobulin (A2M) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. Herein, the function of LvA2M from Litopenaeus vannamei on the prophenoloxidase (proPO) system is reported. The recombinant (r)LvA2M produced strongly and specifically inhibited trypsin and the PO activity in shrimp plasma in a dose-dependent manner. Silencing of LvA2M led to an increase in the PO activity in shrimp plasma although the expression of proPO-associated genes, proPO-activating enzyme (PPAE) and prophenoloxidase (proPO) but not the proPO-activating factor (PPAF) was down-regulated. In Vibrio parahaemolyticus AHPND-infected shrimp, the LvA2M activity was suppressed in an early phase of infection while the PO activity was increased. Thus, the proPO-activating system was regulated by the LvA2M.
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Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge
(2022-11-01)
Chuavong, Wisan
;
Ponprateep, Sirikwan
;
Ajawatanawong, Pravech
;
Vatanavicharn, Tipachai
Polyhydroxybutyrate (PHB) is a natural polymer accumulated in granules as intracellular energy storage in stress condition. The aim of this research was to isolate potential PHB producing bacteria from canned tuna wastewater activated sludge and cow dung. Five positive bacterial isolates were confirmed the accumulation of PHB using lipophilic staining and TEM. To identify the bacterial species, the phylogenetic analyses inferred from 16S rRNA gene sequence demonstrated that the bacterial strains were closely related to Bacillus genera. The comparison of PHB production from bacterial isolates showed that SWU01 strain isolated from the activated sludge exhibited the highest yield of PHB production. The cultivation of SWU01 stain in optimization condition (modified M9 medium supplemented with 2% sodium acetate and 0.5% yeast extract at pH 7 for 48 h) exhibited the yield of PHB up to 0.95 g/L or 7.5 compared with M9 medium supplemented with 2% glucose. Transmission electron micrograph showed high among of PHB in the granule of the bacterial cell. From all results, the bacterial strain SWU01, efficient PHB producers, exhibited the potential for their utilization in commercial PHB production.
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Plasmolipin, PmPLP1, from Penaeus monodon is a potential receptor for yellow head virus infection
(2018-11-01)
Matjank, Watchalaya
;
Ponprateep, Sirikwan
;
Rimphanitchayakit, Vichien
;
Tassanakajon, Anchalee
;
Somboonwiwat, Kunlaya
Plasmolipin has been characterized as a cell entry receptor for mouse endogenous retrovirus. In black tiger shrimp, two isoforms of plasmolipin genes, PmPLP1 and PmPLP2, have been identified from the Penaeus monodon EST database. The PmPLP1 is highly up-regulated in yellow head virus (YHV)-infected shrimp. Herein, the function of PmPLP1 is shown to be involved in YHV infection. The immunoblotting and immunolocalization showed that the PmPLP1 protein was highly expressed and located at the plasma membrane of gills from YHV-infected shrimp. Moreover, the PmPLP1 expressed in the Sf9 insect cells resided at the cell membrane rendering the cells more susceptible to YHV infection. Using the ELISA binding and mortality assays, the synthetic external loop of PmPLP1 was shown to bind the purified YHV and neutralize the virus resulting in the decrease in YHV infection. Our results suggested that the PmPLP1 was likely a receptor of YHV in shrimp.
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Role of cyclophilin A as an immunostimulator in responses to Vibrio parahaemolyticus AHPND infection in the black tiger shrimp Penaeus monodon
(2025-11-01)
Towittayapanchai, Pornsawan
;
Ponprateep, Sirikwan
;
Supungul, Premruethai
;
Tang, Sureerat
;
Jaree, Phattarunda
Cyclophilin A (CypA) is a highly conserved peptidyl-prolyl isomerase that plays a crucial role in protein folding, immune regulation, and cellular stress responses. In shrimp, CypA has been implicated in innate immunity, particularly in response to viral and bacterial infections. This study investigates the role of the Penaeus monodon cyclophilin A (PmCypA) in shrimp infected with V. parahaemolyticus AHPND (VP<inf>AHPND</inf>). During VP<inf>AHPND</inf> infection, the expression levels of PmCypA gene and PmCypA protein were significantly increased at 24 h post-infection (h.p.i.) in shrimp hemocyte. To investigate the role of PmCypA in shrimp immune response, shrimp were administrated with the recombinant PmCypA protein (rPmCypA) and submerged in VP<inf>AHPND</inf> suspension. The results showed that the rPmCypA could rapidly stimulate the expression of immune-related genes involved in several immune pathways, such as immune recognition receptors, clotting system, phagocytosis and homeostasis. Moreover, the injected rPmCypA led to an increase in the proportion of hyaline cells and the greater quantity of granulocytes that contained PmCypA. Furthermore, the rPmCypA could prolong shrimp survival during VP<inf>AHPND</inf> infection. These results suggest that the PmCypA is a bacterial-responsive gene in shrimp immune defense response and potentially an immunostimulator in shrimp.
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Antiviral properties of Penaeus monodon cyclophilin A in response to white spot syndrome virus infection in the black tiger shrimp
(2024-01-01)
Vatanavicharn, Tipachai
;
Matjank, Watchalaya
;
Masrinoul, Promsin
;
Supungul, Premruethai
;
Tassanakajon, Anchalee
Cyclophilin A (CypA) or peptidylprolyl isomerase A, plays an important role in protein folding, trafficking, environmental stress, cell signaling and apoptosis etc. In shrimp, the mRNA expression level of PmCypA was stimulated by LPS. In this study, all three types of shrimp hemocytes: hyaline cell, granulocyte and semi-granulocyte expressed the PmCypA protein. The mRNA expression level of PmCypA was found to be up-regulate to four-fold in white spot syndrome virus (WSSV) infected hemocytes at 48 h. Interestingly, PmCypA protein was only detected extracellularly in shrimp plasma at 24 h post WSSV infection. To find out the function of extracellular PmCypA, the recombinant PmCypA (rPmCypA) was produced and administrated in shrimp primary hemocyte cell culture to observe the antiviral properties. In rPmCypA-administrated hemocyte cell culture, the mRNA transcripts of WSSV intermediate early gene, ie1 and early gene, wsv477 were significantly decreased but not that of late gene, vp28. To explore the antiviral mechanism of PmCypA, the expression of PmCypA in shrimp hemocytes was silenced and the expression of immune-related genes were investigated. Surprisingly, the suppression of PmCypA affected other gene expression, decreasing of penaeidin, PmHHAP and PmCaspase and increasing of C-type lectin. Our results suggested that the PmCypA might plays important role in anti-WSSV via apoptosis pathway. Further studies of PmCypA underlying antiviral mechanism are underway to show its biological function in shrimp immunity.

Vatanavicharn, Tipachai

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