Vatanavicharn, Tipachai

Preferred name

Main Affiliation

School of Science

tipachai.va@kmitl.ac.th

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Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge
(2022-11-01)
Chuavong, Wisan
;
Ponprateep, Sirikwan
;
Ajawatanawong, Pravech
;
Vatanavicharn, Tipachai
Polyhydroxybutyrate (PHB) is a natural polymer accumulated in granules as intracellular energy storage in stress condition. The aim of this research was to isolate potential PHB producing bacteria from canned tuna wastewater activated sludge and cow dung. Five positive bacterial isolates were confirmed the accumulation of PHB using lipophilic staining and TEM. To identify the bacterial species, the phylogenetic analyses inferred from 16S rRNA gene sequence demonstrated that the bacterial strains were closely related to Bacillus genera. The comparison of PHB production from bacterial isolates showed that SWU01 strain isolated from the activated sludge exhibited the highest yield of PHB production. The cultivation of SWU01 stain in optimization condition (modified M9 medium supplemented with 2% sodium acetate and 0.5% yeast extract at pH 7 for 48 h) exhibited the yield of PHB up to 0.95 g/L or 7.5 compared with M9 medium supplemented with 2% glucose. Transmission electron micrograph showed high among of PHB in the granule of the bacterial cell. From all results, the bacterial strain SWU01, efficient PHB producers, exhibited the potential for their utilization in commercial PHB production.
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Production of Herbal Vinegar Using Isolated Microorganisms from Traditional Herbal Vinegar Fermentation
(2023-01-01)
Thongluedee, Rujira
;
Vatanavicharn, Tipachai
;
Sutthiphatkul, Tanyarat
;
Ochaikul, Duangjai
Schizosaccharomyces pombe YM-19 and Acetobacter pasteurianus EM2-03 were isolated and identified from the traditional herbal vinegar fermentation process using cultural and molecular techniques. The two microorganisms were prepared to inoculum for an experimental herbal vinegar fermentation, which is called “Loog Plaag Mea.” The first starter culture was 10% v/v S. pombe YM1-19. Fermentation was carried out under anaerobic conditions at 30°C for 4 days. The alcohol content was 6.18±0.13% v/v and the pH value was 3.52±0.02 on day 4 of the fermentation period. Subsequently, 10% v/v A. pasteurianus EM2-03 was added to the fermentation process under aerobic conditions at 30°C for 22 days. The final herbal vinegar product contained 4.91±0.15% v/v acetic acid, and a pH value of 3.04±0.04. Its total phenolic content and IC<inf>50</inf> value of DPPH radical scavenging were 1,908.38±38.75 µgGAE/mL and 0.017±0.001 µL/mL, respectively. The experimental fermented herbal vinegar had physicochemical properties that very similar to traditional vinegar. As a result, the isolated microorganisms can be used to improve product consistency and quality control in the mass production of vinegar.
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Isolation and identification of fungi with glucoamylase activity from loog-pang-khao-mak (A Thai Traditional Fermentation Starter)
(2020-03-01)
Roongrojmongkhon, Nawaphorn
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Rungjindamai, Nattawut
;
Vatanavicharn, Tipachai
;
Ochaikul, Duangjai
Loog-pang-khao-mak is a Thai traditional fermentation starter that has been used for production of Thai fermented foods for decades. This research aimed to isolate and identify the fungi that produce effective glucoamylase but low ethanol content from the starter. A total of 166 isolates were screened from twelve samples of Loog-pang-khao-mak accumulated from 12 provinces in Thailand using dichloran rose bengal chloramphenicol agar (DRBC Agar). Seventy-nine isolates that effectively hydrolyze starch were selected for glucoamylase activity and alcohol production assay. Three yeast isolates exhibited high glucoamylase activity ranging from 139.14 to 140.94 unit/ml and lowest alcohol yield of 0.41% (v/v) were Saccharomycopsis fibuligera using morphological and molecular identification. The five isolates of mold exhibited high glucoamylase activity (149.20 to 152.60 unit/ml) were identified as Aspergillus Niger, Aspergillus oryzae and Amylomyces rouxii. These findings provide further knowledge on the fungi and their potential use as traditional inocula for fermentation of food products.
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Role of cyclophilin A as an immunostimulator in responses to Vibrio parahaemolyticus AHPND infection in the black tiger shrimp Penaeus monodon
(2025-11-01)
Towittayapanchai, Pornsawan
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Ponprateep, Sirikwan
;
Supungul, Premruethai
;
Tang, Sureerat
;
Jaree, Phattarunda
Cyclophilin A (CypA) is a highly conserved peptidyl-prolyl isomerase that plays a crucial role in protein folding, immune regulation, and cellular stress responses. In shrimp, CypA has been implicated in innate immunity, particularly in response to viral and bacterial infections. This study investigates the role of the Penaeus monodon cyclophilin A (PmCypA) in shrimp infected with V. parahaemolyticus AHPND (VP<inf>AHPND</inf>). During VP<inf>AHPND</inf> infection, the expression levels of PmCypA gene and PmCypA protein were significantly increased at 24 h post-infection (h.p.i.) in shrimp hemocyte. To investigate the role of PmCypA in shrimp immune response, shrimp were administrated with the recombinant PmCypA protein (rPmCypA) and submerged in VP<inf>AHPND</inf> suspension. The results showed that the rPmCypA could rapidly stimulate the expression of immune-related genes involved in several immune pathways, such as immune recognition receptors, clotting system, phagocytosis and homeostasis. Moreover, the injected rPmCypA led to an increase in the proportion of hyaline cells and the greater quantity of granulocytes that contained PmCypA. Furthermore, the rPmCypA could prolong shrimp survival during VP<inf>AHPND</inf> infection. These results suggest that the PmCypA is a bacterial-responsive gene in shrimp immune defense response and potentially an immunostimulator in shrimp.
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Antiviral properties of Penaeus monodon cyclophilin A in response to white spot syndrome virus infection in the black tiger shrimp
(2024-01-01)
Vatanavicharn, Tipachai
;
Matjank, Watchalaya
;
Masrinoul, Promsin
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Supungul, Premruethai
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Tassanakajon, Anchalee
Cyclophilin A (CypA) or peptidylprolyl isomerase A, plays an important role in protein folding, trafficking, environmental stress, cell signaling and apoptosis etc. In shrimp, the mRNA expression level of PmCypA was stimulated by LPS. In this study, all three types of shrimp hemocytes: hyaline cell, granulocyte and semi-granulocyte expressed the PmCypA protein. The mRNA expression level of PmCypA was found to be up-regulate to four-fold in white spot syndrome virus (WSSV) infected hemocytes at 48 h. Interestingly, PmCypA protein was only detected extracellularly in shrimp plasma at 24 h post WSSV infection. To find out the function of extracellular PmCypA, the recombinant PmCypA (rPmCypA) was produced and administrated in shrimp primary hemocyte cell culture to observe the antiviral properties. In rPmCypA-administrated hemocyte cell culture, the mRNA transcripts of WSSV intermediate early gene, ie1 and early gene, wsv477 were significantly decreased but not that of late gene, vp28. To explore the antiviral mechanism of PmCypA, the expression of PmCypA in shrimp hemocytes was silenced and the expression of immune-related genes were investigated. Surprisingly, the suppression of PmCypA affected other gene expression, decreasing of penaeidin, PmHHAP and PmCaspase and increasing of C-type lectin. Our results suggested that the PmCypA might plays important role in anti-WSSV via apoptosis pathway. Further studies of PmCypA underlying antiviral mechanism are underway to show its biological function in shrimp immunity.

Vatanavicharn, Tipachai

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