Koohakan, Prommart

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Koohakan, Prommart
Alternative Name
Koohakan, P.
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Email
prommart.ko@kmitl.ac.th

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    Item type:Publication,
    Morphological characterization and phylogeny of pythium and related genera in rayong province, thailand
    (2021-01-01)
    Saelee, Rattasit
    ;
    Busarakam, Kanungnid
    ;
    Koohakan, Prommart
    Most well-known microorganisms in the class Oomycetes (notably genera Phytophthora and Pythium) are pathogenic to both animals and plants due to their diverse lifestyle patterns. This study was designed to recover Pythium from composite soils (cultivated and forest soils) and water sources (fresh and brackish water) from Rayong Province. Twenty isolates of hyaline and non-septate fungal-like organisms were isolated from those sources. The primer pair ITS4 and ITS6 were used to amplify approximately 900 bp products from Internal transcribed spacer (ITS) region and morphological characteristics including sporangium, oogonium, antheridium and oospore, were noted. Morphological characteristics data of recovered Pythium strain can be classified into 12 source groups. ITS sequencing results revealed that eight closely related species had been recovered: Globisporangium splendens, Pythium cucurbitacearum, Pythium acanthichum, Pythium deliense, Pythium diclinum, Pythium torulosum, Phytopythium vexans and Phytopythium helicoides, which had similarities in the range 94.67-100% values at between 656 and 922 locations. Most of these species were reported as plant pathogens. Therefore, this report can be used as a guide for disease control planning.
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    Item type:Publication,
    Morphological and phylogenetic diversity of Pythium and related genera (Pythiaceae, Pythiales) from some areas in eastern Thailand
    (2022-09-01)
    Saelee, Rattasit
    ;
    Busarakam, Kanungnid
    ;
    Koohakan, Prommart
    Importance of the work: Pythium and related genera in the class Oomycetes are an important microorganism group that affects environmental stability due to their diverse lifestyle patterns. However, full information on their distribution is limited in Thailand. Objectives: To describe their oomycetes distribution in Chanthaburi, Rayong, and Trat provinces, eastern Thailand and provide their diversity and phylogenetic data. Materials & Methods: Composite soils samples were collected July-September 2019. Three techniques (soil baiting, soil dilution and soil plating) were used for isolation. Isolated oomycetes species were obtained from different locations (mangrove forest, natural forest, cultivated field and a river). The isolates were characterized using combined data of morphological traits and sequences analysis of the internal transcribed spacer region (ITS). Results: In total, 52 oomycetes were isolated and assigned to 11 species (Globisporangium carolinianum, Globisporangium splendens, Pythium acanthicum, Pythium deliense, Pythium diclinum, Pythium longipapillum, Pythium myriotylum, Pythium torulosum, Phytopythium cucurbitacearum, Phytopythium helicoides and Phytopythium vexans). Phylogenetic trees of the ITS region analysis indicated that the species obtained from the various locations in this investigation shared a similar biological trait of Pythium and related genera within clades A, B, D, I and K. Main finding: This was the first report of P. longipapillum recovery in Thailand and perhaps the first publication of this species in Thailand and Southeast Asia. Identification and descriptions of Pythium and related genera were provided, as well as useful information to understand their distribution in Thailand's unique ecosystem.