Koohakan, Prommart

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Koohakan, Prommart
Alternative Name
Koohakan, P.
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Email
prommart.ko@kmitl.ac.th

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    Item type:Publication,
    Evaluation of the indigenous microorganisms in soilless culture: Occurrence and quantitative characteristics in the different growing systems
    (2004-05-03)
    Koohakan, Prommart
    ;
    Ikeda, Hideo
    ;
    Jeanaksorn, Tanimnun
    ;
    Tojo, Motoaki
    ;
    Kusakari, Shin Ichi
    Quantitative characteristics such as population density and population dynamics of the indigenous microorganisms in four types of soilless tomato production systems were investigated. For non-specific genera, the amount of the population of aerobic bacteria and fungi in root was 9.2-10 and 4.2-5.5Logcfug<sup>-1</sup>, respectively, regardless of the location of samples collected. For the specific genera, however, fluorescent pseudomonads showed the highest population density (5.4Logcfug<sup>-1</sup>) followed by Fusarium spp. (2.5-3.7Logcfug<sup>-1</sup>) and Pythium spp. (2.3-2.8Logcfug<sup>-1</sup>). Population of microorganisms was significantly different between soilless culture systems as well. The coconut-fiber system (organic substrate culture) showed the highest amount of fungi and Fusarium spp., whereas the rockwool system (inorganic substrate culture) contained the highest amount of fluorescent pseudomonads. Solution culture, DFT and NFT, contained higher amounts of Pythium spp. than the substrate cultures throughout the experiment. NFT also contained the largest population of Fusarium spp. compared to the other ones. In population density investigation, aerobic bacteria in roots became equilibrium at 10Logcfug<sup>-1</sup> in all systems under investigation, however, fungi tended to increase until the end of experiment. The results indicated the unique indigenous microorganisms population in each soilless system. Also, it revealed that aerobic bacteria could be dominant over fungi in inorganic substrate culture. With further research of beneficial microorganisms to horticultural plants, biological control with reduced fungicide application in soilless culture would be feasible. © 2003 Elsevier B.V. All rights reserved.
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    Effects of Inorganic Elements on the In-vitro Growth of Pythium aphanidermatum (Edson) Fitzp
    (2002-01-01)
    Koohakan, Prommart
    ;
    Ikeda, Hideo
    ;
    Jaenaksorn, Tammnun
    ;
    Tojo, Motoaki
    ;
    Kusakari, Shin Ichi
    Effects of inorganic elements on zoospore release, zoospore germination, mycelial growth and sporangium formation of Pythium aphanidermatum were investigated. The stage of zoospore release was highly influenced by many kinds of inorganic elements. The effective concentration as evaluated from 50% inhibition of zoospore release was 0.16, 0.19, 0.36, 0.4, 0.5, 2.5, 30, 168, 171 and 470 mg/L for Cu, Ag, Cr, Zn, iodine, Ni, Fe, Ca, Mg and K, respectively. Silver prominently inhibited both zoospore release and germination. Also, iodine showed inhibitory effects at every growth stage examined. The results indicated that several inorganic elements have the potential to suppress Pythium root rot disease in hydroponics, although further investigation is required to evaluate plants and conditions suitable for the method. © 2002, Japanese Society of Microbial Ecology & The Japanese Society of Soil Microbiology. All rights reserved.
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    Item type:Publication,
    Problems and countermeasures in the re-use of the nutrient solution in soilless production
    (2002-06-30)
    Ikeda, Hideo
    ;
    Koohakan, Prommart
    ;
    Jaenaksorn, Tanimnun
    In this report, problems of pathogen contamination in nutrient solution, imbalance of the minerals, pH and EC control, and accumulation of phytotoxic substances in the solution and the countermeasures for these problems are reviewed. Also, the growing system and the technique of the nutrient solution management for the zero run-off are proposed.