Dawan, Jirapat

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Dawan, Jirapat
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jirapat.da@kmitl.ac.th

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    Item type:Publication,
    Antibiofilm strategies using mixed disinfectants and cubosome nanocarriers to combat Staphylococcus aureus on sandblasted stainless steel disc surfaces
    (2025-09-01)
    Dawan, Jirapat
    ;
    Guo, Yuyuan
    ;
    Lee, Sang Soo
    ;
    Kim, Jin Chul
    Bacterial biofilms are complex communities in which microorganisms are adhered and encapsulated in a self-produced extracellular polymeric material (EPS) matrix. Biofilm acts as a protective barrier, enhancing the resistance of bacteria to environmental stressors, cleaning agents and antimicrobial treatments. Therefore, this study aimed to evaluated the effectiveness of a mixed disinfectant suspension (MIX) against Staphylococcus aureus biofilms on sandblasted stainless steel disc surfaces. A cubic phase containing MIX was prepared, and its anti-biofilm ability was assessed in comparison to free MIX. The susceptibility of S. aureus ATCC 15,564 (SA<sup>ATCC</sup>) and multidrug-resistant S. aureus CCARM 3080 (SA<sup>CCARM</sup>) biofilms to single and mixed disinfectants (MIX) was evaluated. The incorporation of MIX into cubosomes was performed to evaluate its potential enhancement in antibiofilm activity. 1% MIX demonstrated the most effective anti-biofilm activity. Extracellular DNA (eDNA) and polysaccharides were also significantly reduced in biofilms treated with 1% MIX. TEM analysis revealed that 1% MIX effectively disrupted bacterial cell clusters within the biofilms. The incorporation of 1% MIX into cubosomes enhanced its antibiofilm activity, with cubosome-encapsulated MIX exhibiting superior results compared to free MIX. This study demonstrates the effectiveness of a mixed disinfectant suspension in reducing S. aureus biofilms on sandblasted stainless steel disc surfaces. Furthermore, the incorporation of MIX into lipid-based nanocarriers, such as cubosomes, enhanced its antibiofilm efficacy compared to the free disinfectant.