Jantra, Jongjit

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Jantra, Jongjit
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jongjit.jan@kmitl.ac.th

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    Item type:Publication,
    A highly sensitive hyperbranched Au plasmonic blackbody immunochromatographic assay for detection of leucomalachite green in fish and shrimp
    (2024-01-02)
    Teepoo, Siriwan
    ;
    Jantra, Jongjit
    ;
    Panapong, Khaunnapa
    ;
    Ajayi, David Taiwo
    A novel immunochromatographic assay (ICA) based on hyperbranched Au plasmonic blackbodies (AuPBs) with a smartphone readout was fabricated for the detection of leucomalachite green (LMG) in fish and shrimp products. The ICA was carried out in a competitive immunoassay format with AuPBs as labels. The developed AuPBs-ICA allowed for the LMG detection with a low detection limit (0.15 μg L<sup>−1</sup>) within 5 min by the smartphone reader. With the label-AuPBs ICA, the color intensity response was linearly related to the concentrations of the LMG (0.2 −1.7 μg L<sup>−1</sup>). The test line signal could be clearly distinguished at a 1.7 μg L<sup>−1</sup> LMG as a cut-off level by the naked eye, which is lower than the conventional colloidal gold nanoparticle (2 μg L<sup>−1</sup>) and star-shaped nanoparticles (1.9 μg L<sup>−1</sup>) labeling. LMG contamination in samples was determined with the proposed AuPBs-ICA and the enzyme-linked immunosorbent (ELISA). The AuPBs-ICA results showed good agreement with those from the ELISA. The proposed AuPBs-ICA has the potential to be used as a rapid, sensitive, and simple device for the analysis of LMG residues in fish and shrimp samples.