Jantra, Jongjit

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Jantra, Jongjit
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jongjit.jan@kmitl.ac.th

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    Item type:Publication,
    Development of an automated flow-based spectrophotometric immunoassay for continuous detection of zearalenone
    (2020-05-01)
    Jantra, Jongjit
    ;
    Zór, Kinga
    ;
    Sanders, Melanie
    ;
    De Saeger, Sarah
    ;
    Hedström, Martin
    Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an automated sequential injection system. Time for an assay cycle was 18 Min. Under optimal conditions, the limit for quantification for ZEN was 0.40 µg L<sup>−1</sup> with a linear dependency between concentration and signal amplitude between 0.10 and 10 µg L<sup>−1</sup>. The assay proved to be robust and reliable with 13% relative standard deviation between measurements. By dissociating the antigen–antibody complex using a regeneration solution, we showed 50 times reusability of the immobilized antibodies without affecting the antigen-binding properties.