Srisang, Siriwan
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Srisang, Siriwan
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Email
siriwan.sr@kmitl.ac.th
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Item type:Publication, Dip- and Spray-coating of Schanz pin with PLA and PLA nanosphere for prolonged antibacterial activity(2021-10-01); Nasongkla, NorasedIn this study, variation of coating parameters was performed to determine a suitable condition for a vancomycin-coated Schanz pins in poly(lactic acid) (PLA) for prolonged antibacterial activity. Herein, the solvent used to dissolve the polymer (DCM vs. CHCl<inf>3</inf>), the concentration of the polymer in the solvent (2 vs. 5% w/v), the vertical withdrawing speed of the implant from the solution (0.4 vs. 2.4 cm/s), and the number of dip-coating cycles denoted in layers (1 L vs. 3 L) were investigated for its drug loading. Results showed that the optimal parameters were at 2% w/v PLA in DCM at 0.4 cm/s withdrawing speed and at 3 cycles for a high vancomycin loading with low deviation in result. Another experiment was performed by varying the concentration (1, 2, 4% w/v) and number of dip-coating cycles (10 L, 20 L, 30 L) which finalized 2% w/v PLA at 10 cycles as the optimal drug loading at 2.04 ± 0.09 mg. When observing the drug-release profile, the prior formulation released 2.21 ± 0.01 mg or 92.88 ± 0.44% of drug in one week. To reduce the amount of drug release in the first week, the method was further improved by spray-coating with PLA nanospheres (PLA-NS) for multiple cycles (10-, 20-, and 30-NS) to prolong drug release. After one, two, and three weeks, the drug-release were around 76%, 80%, and 88%, respectively. However, more drugs were lost as the number of cycles increases which suggests that lower (10-NS) coating cycle is better. Therefore, the final process was selected to be 2% PLA dip-coated for 10 cycles then spray-coated with PLA nanosphere for 10 cycles. Results showed that this method can help prolong release for at least 24 days. A short-term bacterial and cytotoxicity tests were done for 3 days with no S.aureus formed nor cytotoxicity. These results supported that the coating was successful in inhibiting the growth of S.aureus. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Layer-by-layer dip coating of Foley urinary catheters by chlorhexidine-loaded micelles(2019-02-01); Nasongkla, NorasedChlorhexidine-loaded poly(ethylene glycol)-block-poly(ε-caprolactone) micelles (CHX-micelles) were prepared by the solvent evaporation method and were used to coat urinary catheters. The layer-by-layer (LbL) dip coating technique was used to coat the micelles onto the catheters, which consisted of the alternation of coating with CHX-micelles and poly(acrylic acid). The number of LbL nanocoating cycles was varied, and the properties of the coated catheters were investigated for their drug content and the coating thickness. The optimal coated catheter had 90 bilayers, which provided the maximum CHX content at 32.3 ± 1.4 μg/cm<sup>2</sup>, and the thickness of the thin film on these coated catheters was 6.87 ± 0.36 μm. Coated catheters were studied in the in vitro CHX-release, antibacterial activity, cytotoxicity, and hemolytic activity. Drug release from the coated catheters was controlled, and bacteria proliferation was inhibited for up to 6 days. Results suggest that the antimicrobial activity of the coated catheter reduces the adherence of uropathogens to the catheter's surface. The cytotoxicity and hemolytic activity of the coated catheters showed biocompatible properties. The coated catheter presented the potential to inhibit uropathogen colonization and formation. This nanocoating technology can also be applied to other medical implants that carry the risk of infection. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Spray coating of foley urinary catheter by chlorhexidine-loadedpoly(ε-caprolactone) nanospheres: effect of lyoprotectants, characteristics, and antibacterial activity evaluation(2019-04-21); Nasongkla, NorasedIn this study, chlorhexidine-loaded poly(ε-caprolactone) nanospheres (CHX-NS) were prepared and successfully coated on the urinary catheters. Properties of CHX-NS were evaluated including drug loading content and the nanosphere size. Effects of different lyoprotectants for long-term storage of CHX-NS were also investigated. In vitro release study and antibacterial activity were also conducted using 20 cycles coated-urinary catheters. Results showed that the high-pressure emulsification-solvent evaporation technique provided the drug loading content at 1.14 ± 0.16% and the size of nanospheres was 152 ± 37 nm. The suitable lyoprotectant for long-term storage of CHX-NS was sucrose which provided noticeably no aggregation at the degree of reconstitution at 89.95%. The amount of CHX loading on coated catheters was at 4.55 ± 0.31 mg. Drug release from the coated catheters in artificial urine could be prolonged up to 2 weeks and bacteria proliferation was inhibited up to 14 days. These results suggest that the antimicrobial activity of CHX-NS reduces the adherence of the uropathogens to the catheter surface. Chlorhexidine-loaded polymeric nanospheres were fabricated which can be successfully coated on urinary catheters. These systems have potential use for prolonged antimicrobial applications. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Multilayer nanocoating of Foley urinary catheter by chlorhexidine-loaded nanoparticles for prolonged release and anti-infection of urinary tract(2020-11-21);