Sikhao, Potjana

Preferred name

Sikhao, Potjana

Alternative Name

Sikhao, P.

Main Affiliation

School of Agricultural Technology

potjana.si@kmitl.ac.th

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Film seeds coating with hexane extracts from Illicium verum Hook.f. and Syzygium aromaticum (L.) Merrill & Perry for controlling Callosobruchus maculatus (F.) and Callosobruchus chinensis L
(2021-05-01)
Pumnuan, Jarongsak
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Sarapothong, Kritima
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Sikhao, Potjana
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Pattamadilok, Chutichot
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Insung, Ammorn
BACKGROUND: The application of film seed coating with some plant extracts against stored insect pests was an alternative approach for insect protection instead of using chemical insecticides. The overuse of insecticides has contributed to both human health and environmental problems. The hexane extracts of Illicium verum (HS) and Syzygium aromaticum (HC) were evaluated against bruchid beetles (Callosobruchus maculatus and Callosobruchus chinensis). The insecticidal activities and enhancing seed germination after being coated by various concentrations of extracts, purified fraction (PF) of extracts, standard chemicals of PF, fipronil insecticide (FI), and coating agent groups were evaluated after stored. RESULTS: Both HS and HC at 3% concentrations were more effective against C. maculatus than C. chinensis with LT<inf>50</inf> values of 1.60–1.90 and 3.44–3.62 h, respectively. They could also kill those insects with a higher number than that of standards. The HS and HC could inhibit the progeny population of those insects over 95.6% with no significant difference compared to the FI group. The eugenol standard was the most effective and completely inhibited the emergence compared with trans-anethol standard and PFs. The seed that was coated with 1% of HS and stored at 4 °C for 6 months showed no effect on seed germination with no significant difference between FI and control groups. CONCLUSION: The application of 1% of HS incorporated in the film seed coating against bruchid beetles and did not affect seed germination quality after 6 months. © 2021 Society of Chemical Industry.
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Siamese neem tree as a natural preservative: Chemical profile, antioxidant properties, and antibacterial efficacy against foodborne pathogens and spoilage bacteria
(2025-03-01)
Puttongsiri, Tongchai
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Manichart, Nutcha
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Teerarak, Montinee
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Sikhao, Potjana
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Somala, Naphat
The objective of the present study was to investigate the effect of various ethanol in water solvent systems (0, 25, 50, 75 and 100 % v/v) on the extraction crude yield, total phenolic content, total flavonoid content, and chemical profile of Azadirachta indica (Siamese neem tree) leaf extracts. In addition, the antioxidant and antimicrobial properties were investigated using in vitro assays. Crude yield was highest for the water extract (5.78 g/100 g DW) and decreased with increased ethanol. Total phenolic and flavonoid contents were highest in the 75 % ethanol extract (26.95 mg GAE/g crude and 23.73 mg QE/g crude, respectively). GC-MS analysis of the 75 % ethanol extract revealed 33 bioactive compounds, accounting for 96.33 % of the total area. Significant components included benzene, 1,4-bis(phenylmethyl)- (15.05 %), and naphthalene, 1,6-dimethyl- (10.98 %); also present were sterols, fatty acids, hydrocarbons, aromatic, and heterocyclic compounds, indicating a complex chemical profile. In antioxidant assays, the 75 % ethanol extract revealed the highest DPPH radical scavenging activity (IC<inf>50</inf>: 143.03 mg/L), reducing power (IC<inf>50</inf>: 378.69 mg/L), and lipid peroxidation (IC<inf>50</inf>: 526.25 mg/L). However, the absolute ethanol extract exhibited both the highest metal chelating activity (IC<inf>50</inf>: 382.60 mg/L) and the strongest antimicrobial activity, inhibiting all 11 tested foodborne pathogenic and spoilage bacteria. The lowest minimum inhibitory concentration (MIC) of 25 mg/mL was observed for Staphylococcus aureus, Aeromonas hydrophila, Pseudomonas fluorescens, Latilactobacillus sakei, Streptococcus sp., and Lactococcus cremoris, with inhibition zone diameters of 27.21, 28.79, 28.19, 17.60, 15.09 and 11.20 mm, respectively. Similarly, the lowest minimum bactericidal concentration (MBC) of 25 mg/mL was obtained for S. aureus and L. sakei, indicating potent antibacterial effects against these species. Future studies should include direct comparative analyses of neem extracts and synthetic preservatives to evaluate further their relative efficacy and applicability in real-world food systems.
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Effect of drying methods during priming on quality and longevity of rice seeds
(2022-03-01)
Sangkrabun, P.
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Sikhao, P.
The best suitable drying method for primed Khao Dawk Mali 105 rice seeds was used a hot air oven at 30°C for 36 h and stored them in the uncontrolled environment. There was resulted to be lowest moisture, highest germination percentage and germination index for primed seeds. This method can keep the moisture in rice seeds low, reduce the process of decaying and extend the longevity and quality of primed rice seeds.
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High-Energy Emulsified Clove Essential Oil Nanoemulsion as a Natural Herbicidal Product: Germination Suppression and Seed Structure Alteration in Echinochloa crus-galli
(2026-03-01)
Sikhao, Potjana
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Somala, Naphat
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Manichart, Nutcha
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Dimak, Jantra
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Poonpaiboonpipat, Thanatsan
Clove (Syzygium aromaticum (L.) Merr. & L.M. Perry) essential oil (EO)-based nanoemulsions may have a promising future in eco-friendly herbicide development. Clove EO was found to have a high eugenol content of 87.27%. Organic-solvent-free nanoemulsions using clove EO as a bioactive ingredient were fabricated using ultrasonication and microfluidization emulsification methods. Fourier-transform infrared spectroscopy confirmed that both emulsification methods did not affect the EO components. The droplet size of optimized nanoemulsions was determined using dynamic light scattering. The smallest size of 66.9 nm was obtained by microfluidization at 20,000 psi and eight passes. Additionally, the smallest droplet size for a sonicated nanoemulsion was 103.9 nm, obtained by ultrasonication at 20% for 6 min. Transmission electron microscopy confirmed the droplet sizes of both optimized nanoemulsions. In a storage test, both optimized nanoemulsions were stored at 4 °C for at least four weeks. Finally, both nanoemulsions were evaluated on pre-emergence herbicidal activities against Echinochloa crus-galli. The results showed that both nanoemulsions inhibited E. crus-galli germination and seedling growth, and additionally, inhibited seed imbibition and α-amylase activity. Micro-morphological and ultrastructural analysis was observed using a scanning electron microscope and an energy dispersive X-ray spectrometer (SEM-EDS). SEM-EDS micrographs of the treated seeds showed that the seed structure was damaged, especially the endosperm, leading to the inhibition of seed germination.
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Development of a Melia azedarach L. based natural herbicide: formulation and efficacy studies
(2025-08-01)
Wichittrakarn, Pattharin
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Manichart, Nutcha
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Laosinwattana, Chamroon
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Charoenying, Patchanee
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Sikhao, Potjana
This study evaluated the phytotoxic activity of Melia azedarach L. leaf extract against Echinochloa crus-galli and Phaseolus lathyroides. Crude extracts were obtained using sequential solvent separation, of which the dichloromethane fraction exhibited the highest phytotoxicity for both weed species. However, P. lathyroides appeared more sensitive to the extracts compared to E. crus-galli. GC-MS analysis of the dichloromethane crude extract identified phytol (29.14 %), linolenic acid (21.74 %), and palmitic acid (7.20 %) as the main chemical components. Three formulations, a soluble liquid (SL), pellet, and wettable powder, were developed and evaluated for their effects on seed germination and seedling growth. The SL formulation showed the highest efficacy in inhibiting germination (p < 0.05). Further testing of its pre-emergence utility via soil application in greenhouse conditions confirmed the herbicidal efficacy, effectively suppressing P. lathyroides emergence (36.8 % over control) and reducing growth parameters. Mechanistic assays showed the SL product to affect seed imbibition, α-amylase activity, and cell division in a dose-dependent manner, suggesting these as potential mechanisms of action. This study highlights the potential of a M. azedarach-based SL product as an alternative to synthetic herbicides, potentially leading to the development of a new botanical herbicide.
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Herbicidal Activity of the Invasive Weed Malachra capitata L.: Growth Stage Dependence, Bioassay-Guided Fractionation, and Physiological Effects on Seed Germination
(2026-03-01)
Wichittrakarn, Pattharin
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Sathuwijarn, Sirichai
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Manichart, Nutcha
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Yoneyama, Kaori
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Sikhao, Potjana
The invasive weed Malachra capitata is unsuitable for human or animal consumption but has recently attracted attention for potential alternative uses. In this study, the allelopathic potential of M. capitata for weed control was investigated, as were its allelopathic effects on selected crops. The influence of plant developmental stage on its phytotoxic activity was also assessed. In addition, the physiological effects of the extract on seed germination were investigated. Aqueous leaf extracts were obtained across a range of growth stages and evaluated using seed germination and seedling growth bioassays, followed by bioassay-guided fractionation and GC-MS analysis. Leaves extracts collected at 35 days after planting exhibited the strongest inhibitory activity. Dicot plant species (Phaseolus lathyroides, Cucumis sativus, Brassica oleracea, and B. chinensis) were more susceptible to M. capitata extracts than grassy species (Echinochloa crus-galli, Zea mays, and Oryza sativa), indicating selective phytotoxicity. In pot experiments, application of leaf residues as surface mulch at rates of 100, 200, and 400 g/m<sup>2</sup> significantly reduced P. lathyroides emergence by 11.25%, 35.00%, and 71.25%, respectively. Bioassay-guided fractionation indicated the ethyl acetate-soluble acidic fraction to contain the active allelochemicals. This inhibition was associated with reduced water uptake and suppression of α-amylase activity during seed germination. The most abundant GC-MS detectable components of the acidic fraction were octadecane (12.45%), eicosane (9.74%), and hexadecane (9.60%). Overall, these findings highlight the allelopathic potential of M. capitata, providing a foundation for further applied research and supporting its valorization for sustainable weed management.
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Effect of Seed Coating with Fluorescent Compound on Quality and Fluorescence of Cucumber seeds
(2021-11-01)
Thiphinkong, D.
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Sikhao, P.
The quality and fluorescence of seed after coating with fluorescent compound on cucumber was investigated. The results showed that the coated seed with all types of fluorescent compound had a fluorescence when examined by using a portable ultraviolet light projector. The coated seed with coating substance mixed with Rhodamine had the clearest fluorescence. All the coated seed with coating substance mixed with fluorescence compound were specifically emissions spectra observed from a spectrofluorophotometer. In addition, there were also not affected germination and germination index of seed. In summary, coating with coating substance mixed with Rhodamine is an effective and convenient to mark any cucumber seed lot for provide identity and other qualifications as well as preventing counterfeiting of seeds.
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Application of seed coating with endophytic bacteria for Fusarium wilt disease reduction and growth promotion in tomato
(2020-01-01)
Koohakan, P.
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Prasom, P.
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Sikhao, P.
Tomato seed coating with endophytic bacteria (SuRW02) was used to control Fusarium wilt disease of tomato and stimulated plant growth in field conditions. Results showed that seed coating with isolate SuRW02 reduced disease incidence and severity which similar to seed coating with chemical fungicide (Captan). Seed coating with endophytic bacteria and Captan showed disease incidences of 38 and 25% and disease severity indices of 0.37 and 0.25, respectively. Uncoated seeds and seed coating without endophytic bacteria had a disease incidences of 70 and 50% and disease severity indices of 2.00 and 1.25, respectively. In addition, seed coating with endophytic bacteria promoted tomato plant growth and quality of tomato production.
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A study of polymer for delay germination in hybrid sweet corn seed production
(2024-05-01)
Sodakul, N.
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Sikhao, P.
The results showed that the pH values of each coating substance formulation exhibited statistically significant differences. Notably, the highest pH value was observed in the coating substance mixed with CMC. Similarly, the viscosity of the coating substances showed significant variations, with the commercial coating substance having the highest viscosity. Following this, the coating substances mixed with CMC, HPMC, NaAlg, and PVP-K30 demonstrated progressively lower viscosities. To assess the uniformity of the coating, the surface characteristics of the seeds were visually examined. The results indicated that seeds coated with the commercial coating substance exhibited the highest coating uniformity. Subsequently, the uniformity levels decreased in the following order: seeds coated with a mixture of PVP-K30, HPMC, CMC, and NaAlg. For the seed quality test after coating, the results showed that all types of polymers had no statistically significant effect on the germination percentage and germination index. If further studies are conducted on the types and concentrations of polymers, it is possible to develop seed coating formulations to delay germination.
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Determination of mechanical damage on soybean seed by clorox soak test
(2024-01-01)
Baikaden, S.
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Thepchit, K.
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Buaket, T.
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Sikhao, P.
The Clorox soak test was assessed soybean seed damage, proving to be a comfortable and effective method for detection. Among different seed lots, the third lot exhibited the lowest percentage of damaged seeds. Notably, these seeds yielded seedlings with the highest root length, shoot length, and dry weight. Regarding damage levels, both undropped seeds and those dropped two times displayed the highest shoot length and seedling dry weight. Seed vigor assessed through accelerated aging that showed Lot 2 of soybean seeds had the highest vigor across all damage levels.

Sikhao, Potjana

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