Kongtragoul, Pornprapa
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Preferred name
Kongtragoul, Pornprapa
Alternative Name
Kongtragoul, P.
Main Affiliation
Email
pornprapa.ko@kmitl.ac.th
3 results
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Item type:Publication, ZINC OXIDE NANOPARTICLES INHIBIT SOME FUNGAL PATHOGENS INFECTION ON DURIAN SEEDLINGS(2024-01-01); Pantakan, Miss SansaneeDurian disease-causing organisms such as fungi can infect durian trees at any stage of development. Chemical fungicides are widely used because they are convenient and simple to use. However, it impacts the health of producers, consumers, and the environment. As a result, recommendations have been developed and established for researching zinc oxide nanoparticles (Zn-ONPs) that can inhibit fungal growth as an alternative for preventing and eliminating such fungi. The purpose of this study is to collect and isolate some fungi that cause durian diseases and their pathogenicity test, as well as to investigate the efficacy of Zn-ONPs ranging in size from 25 to 50 nm in inhibiting germination and fungal infection on durian seedlings. Three isolates of Phytophthora sp. (CL4_F1, CL4_S5, CL4_F10), two isolates of Fusarium sp. (CL6_FL1, CL6_FL2), two isolates of Phomopsis sp. (CH1_PF1, CH1_PF2), and two isolates of Rhizoctonia sp. (CL1_RL1, CL1_RL2) were successfully isolated as durian pathogenic fungi. All isolates were pathogenic on durian seedlings, with a 100% disease incidence. As a result, it was found that increasing the concentration of ZnO-NPs could further inhibit growth in the germination and infection of durian seedlings. All tested isolates had delayed germination by ZnO-NPs at all concentrations. Furthermore, as the severity of the disease decreased, ZnO-NPs could inhibit infection in durian seedlings at higher concentrations. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, EFFECT OF ZINC OXIDE NANOPARTICLES AGAINST PHYTOPHTHORA SPP. CAUSING DURIAN DISEASES IN VITRO(2024-01-01); ; Pantakan, SansaneeThe efficiency of zinc oxide nanoparticles (ZnO-NPs) in inhibiting Phytophthora spp. growth in both durian fruits and stems is demonstrated. The size of ZnO-NPs used was in range of 25-50 nm in diameter. Diseased durian sample were collected from a durian orchard located in southern of Thailand. The pathogen of diseased durian was isolated for subsequent morphological identification. Three distinct strains of Phytophthora spp. (designated as PHY41, PHY45, PHY49) were successfully isolated and were found in colonies with rings and stellate pattern, ovoid, limoniform sporangium with semi-papillate or papillate, and formation chlamydospores. All isolates were exhibiting 100% pathogenicity on durian, as indicated by the disease incidence (DI). The concentration of ZnO-NPs played a crucial role in suppressing the growth of Phytophthora spp. in all isolates. Higher concentration of ZnO-NPs resulted in increased inhibition of growth. ZnO-NPs at the concentration of 2000 µg/mL effectively suppressed the mycelial growth of all fungi isolates at 57.76-69.84%. Additionally, ZnO-NPs at the concentration of 500 µg/mL completely inhibited the sporulation only in the PHY49 strains. Furthermore, ZnO-NPs concentration ranging from 1000-2000 µg/mL resulted in completed inhibition in sporulation in all Phytophthora spp. isolates. The result demonstrated that ZnO-NPs at all concentrations effectively delayed the germination of Phytophthora spp. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, In vitro fungicidal effect of chitosan with different molecular weights on fungicide-resistant Phytophthora fruit rot on durian from the export market(2018-08-30)Fruit rots of durian were collected from the export market in Chumphon province, Thailand. The causal organism, Phytophthora spp. was isolated on PAR(PH)-V8 selective medium by tissue transplanting technique. Resistance to dimethomorph, fosetyl-Al, metalaxyl and mancozeb was detected from the mycelial growth on V8 agar amended with fungicide. Multiple fungicide resistant (Mu-F<sup>HR</sup>) and sensitive (Mu-F<sup>S</sup>) isolates are used to study the fungicidal effect of chitosan with different molecular weights. The low, medium and high molecular weight chitosan at 0 (control), 500, 1,000 and 2,000 ppm were evaluated for the development of Mu-FHR and Mu-FS isolates on mycelial growth by poisoned food technique and on sporangium production and zoospores release by culture disc technique. The results indicated that the fungicidal effect of three types of chitosan strengthened as the concentration of chitosan increased for mycelial and sporangium inhibition. However, all chitosan treatments were not significant in zoospores release.