Piyawattanametha, Wibool

Preferred name

Main Affiliation

School of Engineering

wibool.pi@kmitl.ac.th

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Portable holgraphic imager for biological samples
(2014-01-20)
Piyawattanametha, Wibool
A portable holographic imaging platform combining with an automatic micro-objects detection algorithm is demonstrated for biological samples. All components of the imaging platform are aligned to one another along the same optical axis, provides many advantages such as large field-of-view, simple optical setup, and no optical aberration. Main features in the software are feature detection and counting. The average overall computational time is of 52.37 seconds, specificity and sensitivity of our algorithms on a 2593×1944 pixels hologram are 0.978 and 0.923 respectively. Although the algorithm is not optimized, the technique here only focuses on the detection and counting problem.
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Three-dimensional microscopy of biopsies with a handheld confocal microscope
(2015-10-02)
Piyawattanametha, Wibool
Intravital confocal microscopy has provided powerful mechanistic insights into health and disease with three-dimensional imaging capability and has become a common imaging instrument in the modern biological laboratory and clinic. However, the requisite of high numerical aperture, short working distance, and small field of view that enable confocal microscopy limit the ability to investigate hollow organs in vivo. Here we introduce a handheld confocal microscope that circumvents the above technical limitations of confocal microscopy and, as a result, provides imaging access to variety tissues in vivo. The handheld microscope achieves its miniaturization with micro-optics and microelectromechanical systems scanner technology enabling a small form factor and 3-D imaging performance.
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Image mosaicing and real-time imaging with MEMS based handheld confocal microscope
(2014-01-01)
Piyawattanametha, Wibool
In this paper, we demonstrated a handheld confocal fluorescence microscope using dual-axis confocal architecture with a microelectromechanical systems (MEMS) scanner. The laser sources for the microscope are both in visible (660 nm) and near-infrared (778 nm) wavelengths. The microscope will be used for in vivo cervical cancer screening in real-time with human patients. The maximum imaging depth is over 300 μm into the tissue for epithelial cancer screening. The maximum field of view (FOV) is 550 μm × 500 μm with 14 frames/second.
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Centralized pap test diagnosis with artificial neural network and internet of things
(2016-07-02)
Dumripatanachod, Manatchakorn
;
Piyawattanametha, Wibool
In this work, we propose a utilization of a serverclient system model that using the Internet of Things (IoT) technology to process Pap smear imaging data derived from high-resolution microscopes and to classify the those images by employing the Artificial Neural Network (ANN) learning algorithm on the server. The IoT can enable those microscopes to communicate with one another while the ANN enables a new method of imaging classification with high accuracy. We utilize 917 high-resolution images as an input for our proposed method. The method achieves a root mean square error of 0.8834 and correlation coefficient of 0.6643.
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Evaluation of rapid detection system for Escherichia coli in water samples
(2017-02-21)
Pipitsombat, Chanikarn
;
Phasuk, Kanchana
;
Suwan, Supakorn
;
Piyawattanametha, Wibool
We demonstrated a fast detection platform for Escherichia coli (E. coli) in water. The system is based on a fluorescence detection technique by determining enzyme β-D-glucuronidase (GUD) activity and relating that activity to E. coli concentration. We found the portable E. coli detection platform is able to detect both the fluorescence 4-MU signal from the reaction of GUD assay from 0.01-1 μg/ml and to enumerate the number of E. coli in the range of 10<sup>5</sup>-10<sup>7</sup> CFU/ml. The required incubation time is in between 20-240 min. In addition, we can estimate the maximum of incubation time for detecting minimum of E. coli 1 CFU/ml from the equation to be 576 mins. Our preliminary study demonstrates that the system can be used to enumerate the number of E. coli in water on-site and requires less detection time than traditional E. coli methods.
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An ultra-violet sterilization robot for disinfection
(2019-07-01)
Chanprakon, Pacharawan
;
Sae-Oung, Tapparat
;
Treebupachatsakul, Treesukon
;
Hannanta-Anan, Pimkhuan
;
Piyawattanametha, Wibool
Ultraviolet (UV) sterilization technology is used to aid in reduction of microorganisms that may remain on the surfaces after a standard cleaning to the minimum number. Our research team developed a UV robot or UV bot for sterilization in an operating or a patient room. Our UV bot has three 19.3-watt of UV lamps mounted on top of the UV bot platform covering 360° direction. Our UV bot employed an embedded system based on a Raspberry Pi to aid in navigation to avoid obstacles. In addition, we tested the effectiveness of eliminating Staphylococcus Aureus bacteria sample plates located 35 cm away from our UV bot to be within 8 seconds after UV light exposure.
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Isolation of circulating tumor cells under hydrodynamic loading using microuidic technology
(2014-01-01)
Zhao, Cong
;
Lee, Yi Kuen
;
Xu, Rui
;
Liang, Chun
;
Liu, Dayu
Cancer is a leading cause of mortality worldwide causing human deaths. Circulating tumor cells (CTCs) are cells that have detached from a primary tumor and circulate in the bloodstream; they may constitute seeds for subsequent growth of additional tumors (metastasis) in different tissues. The detection of CTCs may have important prognostic and therapeutic implications but, because their number is very small, these cells are not easily detected. Circulating tumor cells are found in the order of 10-100 CTCs per mL of whole blood in patients with metastatic disease. Isolation of tumor cells circulating in the blood stream, by immobilizing them on surfaces functionalized with bio-active coating within microfluidic devices, presents an interdisciplinary challenge requiring expertise in different research areas: cell biology, surface chemistry, fluid mechanics and microsystem technology. We first review the fundamental of cell biology of CTCs and summarize the key microfluidic techniques for isolation of CTCs via cell-ligand interactions, magnetic interactions, filtration; detection and enumeration of CTCs; in vivo CTCs imaging.
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Whole slide imaging based on a low-cost camera
(2018-08-13)
Sasivimolkul, Suvicha
;
Jittichaiwet, Tanakit
;
Piyawattanametha, Wibool
This work presents a new low-cost digital single-lens reflex camera whole slide imaging (DC-WSI) system, which is the most recent imaging modality being employed by pathology departments worldwide due to its high-throughput routine and high-speed capabilities. The DC-WSI system field of view achieves 22.6 times larger than that of the equivalent magnification of a conventional microscope system. The DC-WSI is capable of performing the whole slide scanning (2 cm2) under 2 seconds.
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MEMS-Based Medical Endomicroscopes
(2015-07-01)
Qiu, Zhen
;
Piyawattanametha, Wibool
Progress toward early diagnosis of cancer would have significant clinical benefits in reducing mortality or prolonging life in cancer patients; thus, there is an important unmet clinical need to image cellular features of cancer in vivo and in real time to correlate pathological symptoms and underlying cells responsible for such symptoms. In this paper, we describe a review of microelectromechanical systems scanners-based endoscopic optical coherence tomography, confocal, two-photon, and photoacoustic microscopy imaging. These advanced optical imaging modalities can provide subcellular (micron-scale) resolution and deep tissue penetration to reveal both cells and molecular features for early cancer diagnosis, cancer staging, and surgical guidance.
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MEMS actuators for optical microendoscopy
(2019-01-24)
Qiu, Zhen
;
Piyawattanametha, Wibool
Growing demands for affordable, portable, and reliable optical microendoscopic imaging devices are attracting research institutes and industries to find new manufacturing methods. However, the integration of microscopic components into these subsystems is one of today's challenges in manufacturing and packaging. Together with this kind of miniaturization more and more functional parts have to be accommodated in ever smaller spaces. Therefore, solving this challenge with the use of microelectromechanical systems (MEMS) fabrication technology has opened the promising opportunities in enabling a wide variety of novel optical microendoscopy to be miniaturized. MEMS fabrication technology enables abilities to apply batch fabrication methods with high-precision and to include a wide variety of optical functionalities to the optical components. As a result, MEMS technology has enabled greater accessibility to advance optical microendoscopy technology to provide high-resolution and high-performance imaging matching with traditional table-top microscopy. In this review the latest advancements of MEMS actuators for optical microendoscopy will be discussed in detail.

Piyawattanametha, Wibool

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