Kilaso, Manlika

Preferred name

Kilaso, Manlika

Alternative Name

Kilaso, M.

Main Affiliation

School of Agricultural Technology

manlika.ki@kmitl.ac.th

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Modified atmosphere for thrip disinsection on cut lotus flowers
(2018-01-01)
Bumroongsook, S.
;
Kilaso, M.
In Thailand, lotus flowers are one of exportable products for flower trade industry. Common blossom thrip (Frankliniella schultzei) contamination on cut lotus flowers after harvest has been a major problem for export. F. schultzei is a quarantine insect pest. Modified atmospheres (MA) without oxygen content are considered an alternative to methyl bromide fumigation to control thrips. MA treatment has been used to control insect pests in agricultural product commodities. Different combinations of carbon dioxide, nitrogen, ozone levels, and treatment times were used to effect mortality of the common blossom thrips and postharvest quality of cut lotus flowers. Exposure to different concentrations of O<inf>3</inf> fumigation for 2 days could kill almost all F. schultzei (100% of larvae and ≥ 96% of adults). However, a concentration of 50-250 ppm of O<inf>3</inf> could not control F. schultzei on lotus flowers completely. O<inf>3</inf> fumigation caused color change in lotus flowers. High concentrations of O<inf>3</inf> (≥ 150 ppm) had a negative effect on the visual quality of lotus flowers. The results revealed that thrips mortality increased with increased CO<inf>2</inf> level and storage time. 100% CO<inf>2</inf> caused 100% mortality of both adults and larvae of F. schultzei when there was a 6-h exposure. MA were more effective in disinfecting of thrips on cut lotus flowers after 9 h fumigation to ≥ 50% CO<inf>2</inf> caused complete mortality to F. schultzei. There was not much difference in lotus color in response to atmosphere modified by a combination of CO<inf>2</inf> and N<inf>2</inf>. Therefore, CO<inf>2</inf> disinfestation treatment has the potential to be developed commercially as an alternative postharvest control for common blossom thrips on lotus flowers.
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Life History Characteristic and Larval-Pupal Parasitoids of the Dolichandrone Weevil, Cionus sp. (Coleoptera:Curculionidae)
(2019-01-01)
Kilaso, Manlika
;
Tigwattananont, Saen
;
Bumroongsook, Suvarin
Mangrove trumpet trees (Dolichandrone serrulata (Wall. ex DC.) Seem are popular in Thailand and used as edible plants and ornamental trees. Cionus sp.(Dolichandrone weevil) feeds on Mangrove trumpet trees but data on its life history is very limited. Based on field collection from the Mangrove trumpet tree from public parks in Nonthaburi province, Thailand and laboratory studies, it provides the morphological description of larvae and adults, new data on growth and development of Cionus sp. as well as its larval-pupal parasitoids. The insect species has four different life stages: egg, larva, pupa and adult. After adult emergence for 5-7 days, mating will occur during daytime and the copulation lasts for 5-10 hours. The female laid eggs in a group of 3-7 eggs. A minimum of 14.71 days is required for passing through the egg, larval and pupal stages. Females live slightly longer than males (82.35 and 81.20 days, respectively). Upon the completion of larval development, it will build a cocoon, pupate in there and attach to the ventral or dorsal part of leaf surface. The adults normally emerge in the morning. It exibits death-feigning behaveior when being disturbed. In nature, both adults and larvae are commonly found in the rainy season. These insect larvae feed on leaves and young twig surface of Mangrove trumpet trees whereas adults prefer young leaves. Heavy infestation can stunt the tree’s growth and cause dieback. The larvae and pupae were observed parasitized by the hymenopteran parasitoid, Entedon sp. The parasitization occurrence during May, 2017 to July, 2018 was 6.25-24.19% in larvae and 0.00-62.50 % in papa. Further studies should be conducted to evaluate the efficacy of Entedon sp. as the potential biological control agent for Cionus sp population reduction in home gardens and residential areas.
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Consumption efficiency of Wolf and lynx spiders, pardosa pseudoannulata and oxyopes javanus, on insect pests of asiatic pennywort
(2018-07-01)
Bumroongsook, S.
;
Name, J.
;
Kilaso, M.
Asiatic pennywort is a plant common to Central and Southeast Asia and is used for folk medicine. The most common predatory spiders found in the Asiatic pennywort growing areas were Wolf and lynx spiders, Pardosa pseudoannulata Bösenberg and Strand and Oxyopes javanus Thorell. They were observed the onsuming two important insect pests of pennywort: Young pennywort cutworm, Zonoplusia ochreata Walker, 1865 and a leafhopper, Empoascanara alami Ahmed. The consumption efficiency associated with spider sex and starvation condition of these two predatory spiders on the leafhopper and pennywort cutworm was assessed in the laboratory. The results showed that female spiders consumed more leafhoppers and pennywort cutworms than did the males. Both predatory spiders preferred the second larval instar of pennywort cutworm. The female and male of O. javanus consumed 18.07 and 17.11 insects/day, respectively, and P. pseudoannulata 14.39 and 12.18 insects/day, respectively. Starvation for a prolonged food of time did not increase hunger level of the spiders. The use of these two spiders combined, in comparison with O. javanus alone, against leafhoppers was not significant (P>0.05). However, P. pseudoannulata and O. javanus are important components of the natural enemy complex in pennywort and therefore they should be conserved to aid insect control in pennywort.
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Bionomics of the australian hawk moth, theretra laterillii lucasii (Walker) (Lepidoptera: Sphingidae)
(2018-07-01)
Kilaso, M.
;
Tigvattananont, S.
Theretra latreillii lucasii (Walker) is an important insect pest of water primrose, Ludwigia hyssopifolia (G. Don). Eggs and larvae of T. l. lucasii were collected from L. hyssopifolia and raiseed in the entomological laboratory, King Mongkut's Institute of Technology Ladkrabang. The number of laid eggs was 200-240 eggs per adult female. The egg incubation period lasted 3.47±0.15 days. After egg hatching, T. l. lucasii larvae go through five instars of developmental stages. Duration time of 1st to 5th instars was 3.79±0.50, 4.27±0.57, 3.26±0.50, 3.06±0.32 and 6.59±0.61 days, respectively. The body length was 4.73±1.16, 11.2±1.08, 16.33±1.35, 27.13±1.96 and 53.27±9.19 mm for 1st to 5th instar, respectively. The head capsule for 1st to 5th instar was 0.59±0.08, 0.91±0.06, 1.10±0.030, 2.28±0.15 and 3.87±0.18 mm wide, respectively. The length in mm for dorsal horn of 1st to 5th instar was 2.13±0.72, 3.67±0.62, 5.13±0.58, 5.57±0.46 and 5.63±0.64 mm long, respectively. Adults are medium in sizes with prominent heads and big eyes. The female lived longer than the male (8.40±0.92 and 6.47±0.91days, respectively). The principal host plants of T. l. lucasii are L. hyssopifolia and L. octovalvis in family Onagraceae. Moreover, Cayratia trifolia in family Vitaceae were found to be the food plant for T. l. lucasii.

Kilaso, Manlika

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