Laosinwattana, Chamroon

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Preferred name
Laosinwattana, Chamroon
Alternative Name
Laosinwattana, C.
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Email
chamroon.la@kmitl.ac.th

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    The impact of sodium chloride on root growth, cell division, and interphase silver-stained nucleolar organizer regions (AgNORs) in root tip cells of Allium cepa L.
    (2009-06-17)
    Teerarak, Montinee
    ;
    Bhinija, Kisana
    ;
    Thitavasanta, Sompop
    ;
    Laosinwattana, Chamroon
    To evaluate genetic damage, parameters including root growth, chromosomal aberrations, and interphase silver-stained nucleolar organizer regions (AgNORs) in root tip cells of Allium cepa L. were investigated after treatment with NaCl (40-160 mM). The results showed that NaCl caused a decrease in root growth. All concentrations of NaCl showed an inhibitory effect on dividing cells in root tips of A. cepa L. and caused a reduction in mitotic index values. Upon exposure to NaCl, roots exhibited various mitotic abnormalities, including c-mitosis, anaphase bridge, and chromosome stickiness. In addition, interphase cells with micronuclei, budding nuclei, and unequal-sized nuclei were observed. Moreover, total cell aberration increased with increasing NaCl concentration. For AgNOR parameters, the average number of AgNORs per nucleus decreased in roots treated at all NaCl concentrations. The singular AgNOR area and whole AgNOR area in the nucleus containing 1-3 AgNORs were inversely proportional to NaCl concentrations. © 2009 Elsevier B.V. All rights reserved.
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    Allelopathic potential of Chinese rice flower (aglaia odorata Lour.) as organic herbicide
    (2009-01-01)
    Laosinwattana, C.
    ;
    Poonpaiboonpipat, T.
    ;
    Teerarak, M.
    ;
    Phuwiwat, W.
    ;
    Mongkolaussavaratana, T.
    The aqueous extracts of leaf and branches of Aglaia odorata inhibited the germination and seedling growth of bamyardgrass (Echinochloa crus-galli (L.) Beauv.) and wild pea (Phaseolus lathyroides L.) over the control. Besides its dry leaf powder (31.25, 62.5, 125 and 250 mg/plate) was more inhibitory to these parameters than aqueous extract. Furthermore, the inhibitory effects of dried leaf pellets of Chinese rice flower were more stronger than dried leaf powders at equal rates. The pellets applied at 0.5 ton ha<sup>-1</sup> dose as soil surface mulch inhibited the seedlings emrgence of banyardgrass and wild pea by 67% and 2%, respectively. Thus the Chinese rice flower pellets has the potential to act as organic herbicide for control of bamyardgrass weed.
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    Assessment of allelopathic potential of Vetivergrass (Vetiveria spp.) ecotypes
    (2007-01-01)
    Laosinwattana, C.
    ;
    Phuwiwat, W.
    ;
    Charoenying, P.
    We selected and examined 10 ecotypes of vetivergrass for their allelopathic effects. The allelopatbhic effects of aqueous extracts of each ecotype leaf, rhizome and root plant parts were determined on germination of radish (Raphanus sativas L.). All studied ecotypes inhibited the radish germination and magnitude of their inhibitory effects were ecotypes dependent and varied among plant parts. Nakhon Sawan ecotype was most inhibitory to radish seed germination. Leaves caused strongest inhibition, followed by rhizomes and roots. In another bioassay, the aqueous extracts (concentration of 100 mg/ml) from leaves of Nakhon Sawan ecotype were applied at germination of 15 bioassay plants. Radish, Pearl millet (Pennisetum americanum L.) and Chinese kale (Brasica oleracea var. alboglabra) were the most sensitive to the extract. Besides, a pot experiment showed that Nakhon Sawan leaf residues used as mulch on the soil surface or incorporated into soil reduced the germination and biomass of radish. To isolate inhibitory allelochemicals, methanol extract of Nakhon Sawan ecotype leaf was partitioned into ethyl acetate soluble acidic, neutral and aqueous fractions. The inhibition activities of three fractions were compared with the original extract. The results showed that inhibitory effects increased after solvent partitioning. The original extracts caused 22% inhibition in germination of radish seed at 4,000 ppm, while, ethyl acetate soluble acidic fraction at 4,000 ppm completely inhibited the seed germination. © International Allelopathy Foundation 2007.