Laosinwattana, Chamroon
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Laosinwattana, Chamroon
Alternative Name
Laosinwattana, C.
Main Affiliation
Email
chamroon.la@kmitl.ac.th
13 results
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Item type:Publication, Characterization of Diaporthe fungal extract composition and phytotoxicity on the aquatic noxious weed Eichhornia crassipes: inhibitory effects on photosynthetic machinery and membrane integrity(2025-01-01); ;Manichart, Nutcha ;Thongbang, Muanfan ;Wichittrakarn, PattharinFungal extract-based natural herbicides are a promising avenue for the development of sustainable weed management. The study investigates the herbicidal activity of fungal extracts against the prominent aquatic invasive weed Eichhornia crassipes (water hyacinth). The ethanol crude fraction derived from Diaporthe sp. strain EC010 demonstrated the highest phytotoxicity. Chemical characterization using gas chromatography-mass spectrometry revealed 2(3H)-furanone, dihydro-4-hydroxy- (22.81%), linoelaidic acid (6.87%), and hordenine (6.62%) as major constituents. Phytotoxicity was evaluated by wrapping bioassay under greenhouse conditions. Observable foliar damage, such as necrosis, chlorosis, and depigmentation, occurred within 1 day after treatment (DAT). The rapid onset of visible damage within 1 DAT and the detailed analysis of tissue damage are particularly noteworthy. Lesion progression reflected the phytotoxicity of the extract, increasing to 77.33% visible phytotoxicity at 14 DAT for the highest concentration (8.0% w/v). Microscopic analysis revealed disintegration and complete deformation of epidermal and parenchymal tissues, and treated plants featured extensive aerenchyma spaces. Furthermore, the Diaporthe sp. extract decreased chlorophyll a, b, and carotenoid concentrations while increasing electrolyte leakage and malondialdehyde, indicative of weed deterioration. The current work offers valuable insights for sustainable and eco-friendly strategies in the management of water hyacinth populations in a lentic ecosystem. The findings suggest that Diaporthe sp. extract could serve as a natural herbicide, offering an environmentally friendly alternative to synthetic chemicals in managing water hyacinth. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Potential allelopathic effects of Suregada multiflorum and the influence of soil type on its residue's efficacy(2010-09-01); ;Boonleom, Chaninat; ;Thitavasanta, SompopThis experiment was conducted to determine the potential allelopathic effects of the aqueous extracts and dried leaf powder of Suregada multiflorum and to find the herbicidal effects of its granule on the weed species, barnyardgrass (Echinochloa crus-galli) and slender amaranth (Amaranthus viridis), in various growth media (germination paper, sand, and soil). Observations in this study demonstrated that the potential and magnitude of their inhibitory effects varied among the plant parts. The leaves and branches possessed the strongest inhibition levels, followed by the bark. The aqueous extract from the leaves of S. multiflorum had a remarkably stronger inhibitory effect on the germination and growth of slender amaranth, compared with barnyardgrass; when applied at the concentration of 100-g-L<sup>-1</sup>, it completely inhibited the germination and initial seedling growth of slender amaranth. In addition, all the treatments of the dried leaf powder had stronger inhibition effects than the aqueous extract. Interestingly, the inhibitory effects of the dried leaf granule of S. multiflorum were stronger than the effects of the dried leaf powder at equal rates. The degree of inhibitory effect of the S. multiflorum granule, applied in various growing media, can be classified in order of decreasing inhibition as germination paper = sand > clay soil. The present study indicates that the S. multiflorum granule product that was developed in this research has the potential to provide a powerful organic herbicide for controlling slender amaranth. © 2010 The Authors. Journal compilation © 2010 Weed Science Society of Japan. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, A Potent Phytotoxic Substance in Aglaia odorata Lour.(2016-05-01) ;Kato-Noguchi, Hisashi ;Suzuki, Masahiko ;Noguchi, Kazutaka ;Ohno, OsamuSuenaga, KiyotakeAglaia odorata Lour. (Meliaceae) was found to have very strong allelopathic activity and a bioherbicide PORGANIC<sup>™</sup> was developed from its leaf extracts. However, the phytotoxic substances causing the strong allelopathic activity of the plants have not yet been determined. Therefore, we investigated allelopathic properties and phytotoxic substances in A. odorata. Aqueous EtOH extracts of A. odorata leaves inhibited root and shoot growth of garden cress (Lepidum sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), timothy (Phleum pratense), ryegrass (Lolium multiflorum), and Echinochloa crus-galli with the extract concentration-dependent manner. The extracts were then purified and a major phytotoxic substance with allelopathic activity was isolated and identified by spectral data as rocaglaol. Rocaglaol inhibited the growth of garden cress and E. crus-galli at concentrations > 0.3 and 0.03 μm, respectively. The concentrations required for 50% inhibition ranged from 0.09 to 2.5 μm. The inhibitory activity of rocaglaol on the weed species, E. crus-galli, was much greater than that of abscisic acid. These results suggest that rocaglaol may be a major contributor to the allelopathic effect of A. odorata and bioherbicide PORGANIC<sup>™</sup>. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Inhibitory effects of a variety of aldehydes on Amaranthus tricolor l. And echinochloa crus-galli (L.) beauv.(2018-01-01); ; Thirty-seven commercial aldehydes containing aliphatic chains and aromatic rings as well as heteroaromatic rings were evaluated for their inhibitory activities against Chinese amaranth (Amaranthus tricolor L.) and barnyardgrass (Echinochloa crus-galli (L.) Beauv). Polysorbate 80 (Tween<sup>fi</sup> 80) was used as a surfactant and the research was preliminarily conducted at 400 µM of all aldehydes. Among these aldehydes, (E)-cinnamaldehyde (7) showed the greatest inhibitory effect on seed germination, shoot and root growth of Chinese amaranth by 54.55%, 75.53%, and 85.13% respectively. Similarly, (E)-crotonaldehyde (5), a related α,β-unsaturated aldehyde, inhibited the germination and seedling growth of the tested species at a high percentage. Apart from these two unsaturated aldehydes, no other aliphatic aldehydes had a harmful effect on Chinese amaranth. In terms of benzaldehyde (6), it had no effect on the tested plant; however, many of its derivatives displayed some inhibitory activity. Furthermore, for the ten common heteroaromatic aldehydes, picolinaldehyde (32) had a high inhibitory effect on Chinese amaranth which closely related to the effect of (E)-crotonaldehyde (5) and (E)-cinnamaldehyde (7), whereas, other heteroaromatic aldehydes showed lower effects. In the case of a monocot plant, barnyardgrass, no tested aldehydes reduced seed germination, however, (E)-cinnamaldehyde (7), 2,4,6-trimethoxybenzaldehyde (16) and 4-(dimethylamino)benzaldehyde (24) could inhibit the seedling growth of the plant with low to moderate levels. The herbicidal effects of the most active aldehydes were then further investigated in order to find the minimum concentration of these aldehydes suppressing the germination and growth of the tested plants. At concentrations as low as 50–100 µM some aldehydes could inhibit the seedling growth of the tested species. The structure-activity relationship (SAR) study reported here demonstrates the chemical clues governing the inhibitory activity of aldehydes which could be utilized in the development of highly effective herbicides in the near future. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Development of a Melia azedarach L. based natural herbicide: formulation and efficacy studies(2025-08-01) ;Wichittrakarn, Pattharin ;Manichart, Nutcha; ; This study evaluated the phytotoxic activity of Melia azedarach L. leaf extract against Echinochloa crus-galli and Phaseolus lathyroides. Crude extracts were obtained using sequential solvent separation, of which the dichloromethane fraction exhibited the highest phytotoxicity for both weed species. However, P. lathyroides appeared more sensitive to the extracts compared to E. crus-galli. GC-MS analysis of the dichloromethane crude extract identified phytol (29.14 %), linolenic acid (21.74 %), and palmitic acid (7.20 %) as the main chemical components. Three formulations, a soluble liquid (SL), pellet, and wettable powder, were developed and evaluated for their effects on seed germination and seedling growth. The SL formulation showed the highest efficacy in inhibiting germination (p < 0.05). Further testing of its pre-emergence utility via soil application in greenhouse conditions confirmed the herbicidal efficacy, effectively suppressing P. lathyroides emergence (36.8 % over control) and reducing growth parameters. Mechanistic assays showed the SL product to affect seed imbibition, α-amylase activity, and cell division in a dose-dependent manner, suggesting these as potential mechanisms of action. This study highlights the potential of a M. azedarach-based SL product as an alternative to synthetic herbicides, potentially leading to the development of a new botanical herbicide. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Evaluation of allelopathic, decomposition and cytogenetic activities of Jasminum officinale L. f. var. grandiflorum (L.) Kob. on bioassay plants(2010-07-01); ; Methanolic extracts prepared from dried leaves of Jasminum officinale f. var. grandiflorum (L.) Kob. (Spanish jasmine) inhibited seed germination and stunted both root and shoot length of the weeds Echinochloa crus-galli (L.) Beauv. and Phaseolus lathyroides L. The main active compound was isolated and determined by spectral data as a secoiridoid glucoside named oleuropein. In addition, a decrease in allelopathic efficacy appeared as the decomposition periods increased. The mitotic index in treated onion root tips decreased with increasing concentrations of the extracts and longer periods of treatment. Likewise, the mitotic phase index was altered in onion incubated with crude extract. Furthermore, crude extract produced mitotic abnormalities resulting from its action on chromatin organization and mitotic spindle. © 2010 Elsevier Ltd. All rights reserved. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Allelopathic potential of Chinese rice flower (aglaia odorata Lour.) as organic herbicide(2009-01-01); ;Poonpaiboonpipat, T.; ;Phuwiwat, W.Mongkolaussavaratana, T.The aqueous extracts of leaf and branches of Aglaia odorata inhibited the germination and seedling growth of bamyardgrass (Echinochloa crus-galli (L.) Beauv.) and wild pea (Phaseolus lathyroides L.) over the control. Besides its dry leaf powder (31.25, 62.5, 125 and 250 mg/plate) was more inhibitory to these parameters than aqueous extract. Furthermore, the inhibitory effects of dried leaf pellets of Chinese rice flower were more stronger than dried leaf powders at equal rates. The pellets applied at 0.5 ton ha<sup>-1</sup> dose as soil surface mulch inhibited the seedlings emrgence of banyardgrass and wild pea by 67% and 2%, respectively. Thus the Chinese rice flower pellets has the potential to act as organic herbicide for control of bamyardgrass weed. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Physiological and cellular mechanisms of natural herbicide resource from Aglaia odorata Lour. On bioassay plants(2012-07-01); ; A bioassay-guided fractionation of the allelochemicals of the ethyl acetate fraction extracted from Aglaia odorata led to isolation of a bisamide, odorine. The growth inhibitory effects of odorine and ethyl acetate fraction were studied for comparison on Echinochloa crus-galli. Odorine and ethyl acetate fraction of A. odorata could inhibit the germination and seedling growth of E. crus-galli, with ethyl acetate fraction being more potent. Thus, ethyl acetate fraction was selected for further experiment, E. crus-galli seeds were studied the effects of the ethyl acetate fraction from A. odorata leaves in wet-table powder formulation on imbibition and α-amylase activity. It was found that treated seeds showed lower imbibition and α-amylase activity. The results of cytogenetic bioassay in Allium cepa roots showed that ethyl acetate fraction inhibited cell mitosis and induced mitotic abnormalities resulting from its action on chromatin organization and mitotic spindle in the exposed roots. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, An allelopathic substance isolated from Zanthoxylum limonella Alston fruit(2010-06-28); ; Bioassay-guided fractionation of the ethyl acetate extract of the Zanthoxylum limonella Alston fruits led to the isolation of a phenolic compound named xanthoxyline. The allelopathic effects of xanthoxyline were evaluated on Chinese amaranth (Amaranthus tricolor L.) and Barnyardgrass (Echinochloa crus-galli (L.) Beauv.) under laboratory conditions. At a concentration of 2500 μM, xanthoxyline completely inhibited seed germination and growth of Chinese amaranth, while this compound showed a significantly inhibitory effect on seed germination of Barnyardgrass by 43.59% and inhibited shoot and root length by 71.56% and 87.48%, respectively. To determine the mechanism through which xanthoxyline exerts its toxicity, the cytogenetic bioassay was conducted. Roots of Allium cepa L. (onion) were treated with a series of concentrations, ranging from 15.625 to 125 μM for 18 h. Cytogenetic studies of onion roots showed a decrease of the mitotic index in the exposed roots dependent on the concentration of xanthoxyline. Likewise, the mitotic phase index was altered in onion incubated with xanthoxyline. Analyses of the abbreviation types showed spindle disturbances at late prophase, sticky metaphase, c-mitosis, non-oriented chromosome at metaphase, sticky anaphase, delayed anaphase and diagonal at telophase for the onion roots exposed to xanthoxyline. © 2010 Elsevier B.V. All rights reserved. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Optimal extraction solvents use for extraction of Thunbergia laurifolia Linn. Leaves and its mode of action on weed control(2018-12-01) ;Thinh, N. H.; Wichittrakarn, P.Thunbergia laurifolia Lindl. leaves were extracted by various ratios (0, 25, 50, 75 and 100%) of ethanol in water as solvents for crude extraction. The highest crude extract yield was obtained by using 25% ethanol solvent with three time extractions. The inhibitory effect of each extract on seed germination and seedling growth of Amaranthus gracilis was investigated. The results indicated that the 100% ethanol extract expressed the best inhibitory effect. At concentration of 1250 ppm, the 100% ethanol extract completely inhibited seed germination of A. gracilis. The 100% ethanol extract also inhibited α-amylase activities in A. gracilis seed during seed germination. However, the extract does not show effect on seed imbibition. The inhibition of induction of α-amylase increased with increasing concentration of the 100% ethanol extract.
