Maneeruttanarungroj, Cherdsak

Preferred name

Main Affiliation

School of Science

cherdsak.ma@kmitl.ac.th

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Upregulation of Hox-hydrogenase gene expression by nutrient adjustment in the filamentous non-heterocystous cyanobacterium Arthrospira sp. PCC 8005
(2020-12-01)
Raksajit, Wuttinun
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Maneeruttanarungroj, Cherdsak
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Mäenpää, Pirkko
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Lehto, Kirsi
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Incharoensakdi, Aran
Arthrospira sp. PCC 8005 is potentially able to produce hydrogen catalysed by hox gene–encoded bidirectional hydrogenase with the aid of hyp gene–encoded accessory proteins. In the present study, we investigated the physiological factors affecting the hoxE, hoxY, hoxH, and hypF transcription in Arthrospira sp. PCC 8005. About a 4-fold increase of biomass and chlorophyll-a content was observed in cells grown for 7 days in Zarrouk’s medium supplemented with Fe2+. Cells grown in N-deprived medium with added Ni2+ had increased H<inf>2</inf> production and hydrogenase activity with a maximal value of 7.24 ± 0.25 μmol H<inf>2</inf> mg−1 Chla h−1 and 0.61 ± 0.03 μmol H<inf>2</inf> mg−1 Chla h−1, respectively. RT-PCR analysis revealed that cells grown in the N-deprived medium supplemented with Fe2+ or Ni2+ increased hoxE, hoxY, and hoxH transcripts. However, the highest increase of the hoxE, hoxY, hoxH, and hypF transcripts was observed in cells grown in the S-deprived medium supplemented with a combination of Fe2+ and β-mercaptoethanol. These results indicated that the increased expression of hox genes in Arthrospira sp. PCC 8005 can be achieved by proper adjustment of the nutrients in the growth medium. Phylogenetic analysis revealed that the small hydrogenase subunit, HoxY sequence, from Arthrospira sp. PCC 8005 was clustered together along with other cyanobacterial HoxY which is highly related to Arthrospira platensis NIES46.
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Photobiohydrogen Production and Strategies for H2 Yield Improvements in Cyanobacteria
(2023-01-01)
Khetkorn, Wanthanee
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Raksajit, Wuttinun
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Maneeruttanarungroj, Cherdsak
;
Lindblad, Peter
Hydrogen gas (H<inf>2</inf>) is one of the potential future sustainable and clean energy carriers that may substitute the use of fossil resources including fuels since it has a high energy content (heating value of 141.65 MJ/kg) when compared to traditional hydrocarbon fuels [1]. Water is a primary product of combustion being a most significant advantage of H<inf>2</inf> being environmentally friendly with the capacity to reduce global greenhouse gas emissions. H<inf>2</inf> is used in various applications. It generates electricity in fuel cells, including applications in transportation, and can be applied as fuel in rocket engines [2]. Moreover, H<inf>2</inf> is an important gas and raw material in many industrial applications. However, the high cost of the H<inf>2</inf> production processes requiring the use of other energy sources is a significant disadvantage. At present, H<inf>2</inf> can be prepared in many conventional ways, such as steam reforming, electrolysis, and biohydrogen production processes. Steam reforming uses high-temperature steam to produce hydrogen gas from fossil resources including natural gas. Electrolysis is an electrolytic process to decompose water molecules into O<inf>2</inf> and H<inf>2</inf>. However, both these two methods are energy-intensive and producing hydrogen from natural gas, which is mostly methane (CH<inf>4</inf>) and in steam reforming generates CO<inf>2</inf> and pollutants as by-products. On the other hand, biological hydrogen production is more environmentally sustainable and less energy intensive than thermochemical and electrochemical processes [3], but most concepts are not yet developed to production scale.
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Response of green alga Tetraspora sp. CU2551 under potassium deprivation: a new promising strategy for hydrogen production
(2022-04-01)
Pewnual, Totsapon
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Jampapetch, Narirat
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Saladtook, Sathonkorn
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Raksajit, Wuttinun
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Klinsalee, Rachaneekorn
With increasing world energy consumption, renewable energy sources can fulfill the need and many have net zero carbon dioxide emissions. One of these is hydrogen, which is biologically produced molecular hydrogen from organisms. Although many studies have produced hydrogen from green algae and optimized conditions to obtain the highest yield, following our previous works on production optimization from Tetraspora sp. CU2551, we tested deprivation of Fe, Cu, Ca, S, Mg, or K, and surprisingly found that K deprivation showed another promising switch in increased H<inf>2</inf> yield, representing 9.2 ± 0.1 μmol mg−1 DW within 32 h incubation anaerobically. Moreover, longer cell adaptation by aerobic incubation, before production phase, resulted in higher hydrogen yield. Cells adapted in potassium deprivation (TAP-K medium) promoted hydrogen production to a yield of 14.8 ± 0.03 μmol mg−1 DW, or about 3.0 times higher than normal TAP (5.0 ± 0.82 μmol mg−1 DW). The increased yield was caused by (1) lowering PSII activity (direct biophotolysis), resulted in less oxygen being produced, lowering hydrogenase inhibitor levels, and (2) increasing accumulated starch degradation (indirect biophotolysis) (12.2% starch remaining or 7.2 ± 0.9 μg mg−1 DW), increased the flow of electrons to hydrogenase. Our finding makes potassium deprivation conditions as another the promising choice to enhance hydrogen production in biological systems.
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Molecular detection and phylogeny of Ehrlichia canis and Anaplasma platys in naturally infected dogs in Central and Northeast Thailand
(2022-12-01)
Purisarn, Andaman
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Wichianchot, Sakulchit
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Maneeruttanarungroj, Cherdsak
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Mangkit, Bandid
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Raksajit, Wuttinun
Background and Aim: Ehrlichia canis and Anaplasma platys are tick-borne, Gram-negative bacteria that cause canine monocytic ehrlichiosis and canine cyclic thrombocytopenia, respectively. These diseases are of great importance and are distributed globally. This study aimed to create new primers for the identification of E. canis and A. platys in naturally infected dogs using polymerase chain reaction (PCR), DNA sequencing, and phylogenetic analysis using the 16S rDNA and gltA genes. Materials and Methods: In total, 120 blood samples were collected from dogs in three different locations (Saraburi, Buriram, and Nakhon Ratchasima provinces) in Central and Northeast Thailand. The molecular prevalence of E. canis and A. platys was assessed using PCR targeting the 16S rDNA and gltA genes. All positive PCR amplicons were sequenced, and phylogenetic trees were constructed based on the maximum likelihood method. Results: Ehrlichia canis had an overall molecular prevalence of 15.8% based on the 16S rDNA gene, compared to 8.3% based on the gltA gene. In addition, the overall molecular prevalence of A. platys using the 16S rDNA gene was 10.8%, while the prevalence rate was 5.8% using the gltA gene. Coinfection was 0.8% in Saraburi province. The partial sequences of the 16S rDNA and gltA genes of E. canis and A. platys in dogs in Central and Northeast Thailand showed 96.75%–100% identity to reference sequences in GenBank. Phylogenetic analysis of the 16S rDNA and gltA genes revealed that E. canis and A. platys sequences were clearly grouped into their own clades. Conclusion: This study demonstrated the molecular prevalence of E. canis and A. platys in Central and Northeast Thailand. The 16S rDNA and gltA genes were useful for the diagnosis of E. canis and A. platys. Based on the phylogenetic analysis, the partial sequences of the 16S rDNA and gltA genes in E. canis and A. platys were related to prior Thai strains and those from other countries.
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Optimal conditions for maximized H2 yield from a new green algal strain Chlorella sp. KLSc61
(2022-08-01)
Laokua, Natwikar
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Rittiyan, Nutnicha
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Kornrawudaphikasama, Yosita
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Klinsalee, Rachaneekorn
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Tonawut, Yothawut
Hydrogen produced from microalgae is attracting the attention of scientists as a potential new ‘Bio-Circular-Green’ energy source. We screened a selection of naturally occurring algal strains for H<inf>2</inf> yield as a basis for high yield development. The best strain was a new strain of biohydrogen-producing green algae from a fresh water source at King Mongkut's Institute of Technology Ladkrabang, Thailand. Using morphological and 18S rDNA sequencing analyses, this alga was identified and classified as Chlorella sp. KLSc61. When adapted cells in TAP-K medium (with starting pH at 9.0) containing 25 mM ethanol, with 54 μmol photons m−2 s−1 light, and incubated at 35 °C, Chlorella sp. KLSc61 showed a maximum H<inf>2</inf> yield of 9,804 ± 469.5 μmol H<inf>2</inf> mg−1 Chl at day 7. Under these conditions, the cells produced ~ 12.2 times more H<inf>2</inf> gas than without optimization (753.0 μmol H<inf>2</inf> mg−1Chl). Our optimized system also produced H<inf>2</inf> at a rate 11–570 times greater than previously reported microalgae. Thus Chlorella sp. KLSc61 could be a viable strain for biohydrogen production as a clean future energy source.
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Characterization and exploration of biological properties of phycobiliproteins purified from Thai karstic cave cyanobacterium Nostoc sp. SW02
(2023-09-01)
Suphan, Sutthawan
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Limrujiwat, Kittakorn
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Kula, Kasinee
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Maneeruttanarungroj, Cherdsak
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Raksajit, Wuttinun
Phycobiliproteins are colored water-soluble proteins found in cyanobacteria with potential applications in food and pharmaceuticals. Phycobiliproteins extracted from the Thai karstic cave cyanobacterium Nostoc sp. strain SW02 were purified, and their biological properties were characterized. Scale-up cultivation for 12 days revealed that maximum biomass and phycobiliproteins producing yield were 1.53 g/L and 31.92%, respectively. After purification, phycoerythrin was obtained as the principal component of phycobiliproteins, with a purity index up to 2.62, sufficient to be considered food and cosmetic grade. Native and SDS-PAGE analysis displayed that purified phycoerythrin and phycocyanin contained the αβ-subunits in hexamer form. Purified phycobiliproteins had a more stable structure and functionality in pH range 5.0–7.0, were resistant to strong oxidizers, and could withstand temperatures up to 60 °C. They also exhibited DPPH scavenging capabilities comparable to standard ascorbic acid. Pathogenic bacterial inhibition (MIC 250 μg/mL) and anticancer activity against the human HeLa cancer cell line (IC<inf>50</inf> 140 μg/mL) were also observed. With these prospective characteristics, phycobiliproteins obtained from Nostoc sp. SW02 have biotechnological value for biomedical research and also as a natural colorant for food and cosmetic applications.
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Exogenous Trehalose Improves Growth, Glycogen and Poly-3-Hydroxybutyrate (PHB) Contents in Photoautotrophically Grown Arthrospira platensis under Nitrogen Deprivation
(2024-02-01)
Mudtham, Nat Anong
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Promariya, Authen
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Duangsri, Chanchanok
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Maneeruttanarungroj, Cherdsak
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Ngamkala, Suchanit
Glycogen and poly-3-hydroxybutyrate (PHB) are excellent biopolymer products from cyanobacteria. In this study, we demonstrate that nitrogen metabolism is positively influenced by the exogenous application of trehalose (Tre) in Arthrospira platensis under nitrogen-deprived (−N) conditions. Cells were cultivated photoautotrophically for 5 days under −N conditions, with or without the addition of exogenous Tre. The results revealed that biomass and chlorophyll-a content of A. platensis experienced enhancement with the addition of 0.003 M and 0.03 M Tre in the −N medium after one day, indicating relief from growth inhibition caused by nitrogen deprivation. The highest glycogen content (54.09 ± 1.6% (w/w) DW) was observed in cells grown for 2 days under the −N + 0.003 M Tre condition (p < 0.05), while the highest PHB content (15.2 ± 0.2% (w/w) DW) was observed in cells grown for 3 days under the −N + 0.03 M Tre condition (p < 0.05). The RT-PCR analysis showed a significant increase in glgA and phaC transcript levels, representing approximately 1.2- and 1.3-fold increases, respectively, in A. platensis grown under −N + 0.003 M Tre and −N + 0.03 M Tre conditions. This was accompanied by the induction of enzyme activities, including glycogen synthase and PHA synthase with maximal values of 89.15 and 0.68 µmol min−1 mg−1 protein, respectively. The chemical structure identification of glycogen and PHB from A. platensis was confirmed by FTIR and NMR analysis. This research represents the first study examining the performance of trehalose in promoting glycogen and PHB production in cyanobacteria under nitrogen-deprived conditions.

Maneeruttanarungroj, Cherdsak

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