Thawai, Chitti

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Thawai, Chitti
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chitti.th@kmitl.ac.th

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    Item type:Publication,
    Nocardia coffeae sp. nov., an endophytic actinobacterium isolatefrom the root of Coffea arabica (L.)
    (2022-01-01)
    Nammali, Achararak
    ;
    Duangupama, Thitikorn
    ;
    Suriyachadkun, Chanwit
    ;
    Tanasupawat, Somboon
    ;
    Thawai, Chitti
    The polyphasic taxonomic study of a novel endophytic actinobacterium strain (CA2R105<sup>T</sup>) was carried out. The strain formed fragmented substrate mycelium and showed chemotaxonomic properties typical of members of the genus Nocardia, i.e. the presence of mycolic acid and MK-8 (H<inf>4ω-cycl</inf> ) in its cells. Strain CA2R105<sup>T</sup> exhibited the highest 16S rRNA gene sequence similarity to Nocardia jiangxiensis NBRC 101359<sup>T</sup> (99.2%). The genome-based taxonomic analysis revealed low average nucleotide identity-blast and digital DNA–DNA hybridization values (<93.7, and <65.2%, respectively) to its closest relative. Moreover, many different phenotypic characteristics were observed between strain CA2R105<sup>T</sup> and all related Nocardia-type strains. This taxonomic evidence suggested that strain CA2R105<sup>T</sup> should be judged as representing a novel species of the genus Nocardia and the name, Nocardia coffeae sp. nov. is proposed. The type strain is CA2R105<sup>T</sup> (=TBRC 11247<sup>T</sup>=NBRC 114292<sup>T</sup>).
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    Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)
    (2021-11-01)
    Nammali, Achararak
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110<sup>T</sup>, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110<sup>T</sup> was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541<sup>T</sup> (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491<sup>T</sup> (99.1%), Streptomyces luteoverticillatus NBRC 3840<sup>T</sup> (99.1%), Streptomyces coerulescens NBRC 12758<sup>T</sup> (99.1%), and Streptomyces iranensis HM 35<sup>T</sup> (99.0%). Strain CA3R110<sup>T</sup> contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H<inf>6</inf>), and MK-9(H<inf>8</inf>) as major menaquinone, iso-C<inf>16:0,</inf>iso-C<inf>15:0,</inf> C<inf>16:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110<sup>T</sup> could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110<sup>T</sup> represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110<sup>T</sup> (= TBRC 11245<sup>T</sup> = NBRC 114296<sup>T</sup>).
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    Item type:Publication,
    Streptomyces coffeae sp. Nov., an endophytic actinomycete isolated from the root of coffea arabica (l.)
    (2021-01-01)
    Nammali, Achararak
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An endophytic actinobacterium, designated strain CA1R205<sup>T</sup>, was isolated from the surface-sterilized root of Coffea arabica L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. The strain produced light yellowish brown to dark brownish black substrate mycelium and greyish white aerial mycelium. The spiral spore chains were produced directly on aerial mycelium. CA1R205<sup>T</sup> was found to have ll-diaminopimelic acid in the cell peptidoglycan, galactose, glucose, mannose and ribose as whole-cell reducing sugars, MK-10(H<inf>4</inf>), MK-9(H<inf>6</inf>), MK-10(H<inf>2</inf>), MK-9(H<inf>4</inf>), MK-10(H<inf>6</inf>) and MK-10(H<inf>8</inf>) as menaquinones and iso-C<inf>15:0</inf>, anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf> and C<inf>16:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were detected in the cells. These characteristics were consistent the typical chemotaxonomic properties of members the genus Streptomyces. The taxonomic affiliation at the genus level of this strain could be confirmed using its 16S rRNA gene sequence data. CA1R205<sup>T</sup> showed the highest 16S rRNA gene sequence similarity value to Streptomyces rapamycinicus NRRL B-5491<sup>T</sup> (98.9%), followed by Streptomyces iranensis HM 35<sup>T</sup> (98.8%). Digital DNA–DNA hybridization and average nucleotide identity-by blast (ANIb) values between CA1R205<sup>T</sup> and S. rapamycinicus NRRL B-5491<sup>T</sup> were 27.2 and 81.5%, respectively. The DNA G+C content of genomic DNA was 70.7 mol%. Due to the differences in physiological, biochemical and genotypic data, CA1R205<sup>T</sup> could be discriminated from its closest neighbour. Thus, CA1R205<sup>T</sup> should be recognized as representing a novel species of the genus Streptomyces, for which the name Streptomyces coffeae sp. nov. is proposed. The type strain is CA1R205<sup>T</sup> (=TBRC 11244<sup>T</sup>=NBRC 114295<sup>T</sup>).
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    Item type:Publication,
    Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans
    (2019-02-01)
    Niemhom, Nantawan
    ;
    Chutrakul, Chanikul
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    ;
    Thawai, Chitti
    An endophytic actinobacterium, designated strain KE2-3 <sup>T</sup> , was isolated from surface-sterilised rhizome of Kaempferia elegans. The polyphasic approach was used for evaluating the taxonomic position of this strain. The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of LL-diaminopimelic acid in the cell peptidoglycan, MK-9(H <inf>4</inf> ) as the major menaquinone, iso-C <inf>16: 0</inf> , anteiso-C <inf>15: 0</inf> , iso-C <inf>14: 0</inf> and iso-C <inf>15: 0</inf> as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes. Based on 16S rRNA gene sequence analysis, strain KE2-3 <sup>T</sup> was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357 <sup>T</sup> (99.3 %) followed by Jiangella alba DSM 45237 <sup>T</sup> (99.2 %), Jiangella alkaliphiliaDSM 45079 <sup>T</sup> (99.0 %), Jiangella gansuensis DSM 44835 <sup>T</sup> (98.8 %) and Jiangella mangrovi 3SM4-07 <sup>T</sup> (98.6 %). However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96%species circumscription threshold. The DNA G+C content of genomic DNA was 72.3 mol%. With the differences of physiological, biochemical and genotypic data, strain KE2-3 <sup>T</sup> could be discriminated from its closest neighbour. Thus, strain KE2-3 <sup>T</sup> should be recognised as a novel species of genus Jiangella, for which the name Jiangella endophytica sp. nov. is proposed. The type strain is KE2-3 <sup>T</sup> (=BCC 66359 <sup>T</sup> =NBRC 110004 <sup>T</sup> ).