Keeratirawee, Kanchalar

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Preferred name
Keeratirawee, Kanchalar
Alternative Name
Keeratirawee, K.
Main Affiliation
Email
kanchalar.ke@kmitl.ac.th

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Now showing 1 - 4 of 4
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    Synergistic effect of salicylhydroxamic acid in quinone outside inhibitor fungicides and its sensitivity on fruit rot pathogens
    (2024-01-01)
    Keeratirawee, K.
    ;
    Kongtragoul, P.
    The synergistic effect of treatment with both salicylhydroxamic acid (SHAM) and treatment with azoxystrobin (AZ), trifloxystrobin (TF), and pyraclostrobin (PR) which are present in Ool (quinone outside inhibitor) on fruit rot pathogens was investigated. Twenty-five fungal pathogen isolates were collected from chili, mango, and durian fruit rot. Ten isolates of Phytophthora palmivora were obtained from durian fruits and 15 Colletotrichum spp. were obtained from chili and mango fruits. The efficiency of SHAM interaction with AZ, TF, and PR at various concentrations to mycelial growth was statistically defined by a synergy factor (SF). A value of a SF above 1 represented the synergism of SHAM and fungicide in QoI to inhibit fungi respiration. The fungicide sensitivity was evaluated with the classification of the levels of sensitivity based on 50% effective concentrations (EC<inf>50</inf>). All tested isolates were susceptible to QoI fungicides at EC<inf>50</inf> lower than 10 mg L<sup>-1</sup>. Only one isolate of Colletotrichum sp. from chili (CC_P003) was intermediately resistant to AZ at 16.52 mg L<sup>-1</sup> EC<inf>50</inf>.
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    Preparation of Low-Cost Activated Carbon from Spent Coffee Grounds for Rhodamine B Removal
    (2025-01-01)
    Keeratirawee, Kanchalar
    ;
    Choola-aied, Orasa
    ;
    Kongtragoul, Pornprapa
    A low-cost coffee-based activated carbon for Rhodamine B (RhB) removal was investigated. Spent coffee grounds obtained from a local coffee shop in Pathio, Chumphon, Thailand, were utilized as the raw material to produce biochar. The coffee grounds were pre-treated by washing with 1M H2SO4 before carbonization at 600 ºC for 2 h under a limit-oxygen atmosphere. The acid washing process was found to be a promising method for preparing activated carbon without requiring an inert gas flow during the carbonization. This approach resulted in activated carbon free of ash after carbonization. The carbonized coffee grounds were activated using a microwave-assisted process with KOH as the activating agent. The performance of the prepared activated carbon for Rhodamine B adsorption was evaluated using the batch method. Key parameters, including initial concentration of Rhodamine B (3-11 mg/L), pH (3-11), and contact time (15-150 min), were optimized. The maximum adsorption capacity was 16 mg/g of adsorbents. The optimal pH for Rhodamine B adsorption was found to be 7. The adsorption of Rhodamine B onto the adsorbent fits a Langmuir isotherm and a pseudo-second-order kinetic model. The study revealed that the prepared activated carbon from waste coffee grounds is an efficient and affordable solution for removing Rhodamine B contaminants from the water system.
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    Low-Cost Activated Carbon for Methylene Blue Adsorption Using Inert Gas-Free Carbonization and Microwave Activation
    (2025-01-01)
    Kongtragoul, Pornprapa
    ;
    Keeratirawee, Kanchalar
    ;
    Choola-Aied, Orasa
    -This study investigated the preparation of activated carbon from spent coffee grounds for methylene blue adsorption using inert gas-free carbonization and microwave activation. Spent coffee grounds were obtained from a local coffee shop in Pathio, Chumphon, Thailand. The coffee grounds were pretreated with 1M H<inf>2</inf>SO<inf>4</inf> before undergoing carbonization at 600 ºC for 3 h in a limited-oxygen atmosphere. The obtained carbon materials were chemically activated with zinc chloride (ZnCl<inf>2</inf>) and physically activated using a microwave-assisted method at 300 W for 180 seconds. The adsorption of methylene blue was evaluated using a batch method. Methylene blue adsorption contact time, effect of pH, and initial concentration were investigated. The maximum adsorption capacity of the activated carbon for methylene blue removal was found to be 29.24 mg/g. The prepared activated carbon exhibited a BET surface area of 70.08 m<sup>2</sup>/g and reached equilibrium in 120 min at the optimum pH of 9. The adsorption isotherm was consistent with the Freundlich model, while the adsorption kinetics followed a pseudo-second-order model. The findings indicate that activated carbon is a cost-effective and efficient adsorbent for methylene blue removal from aqueous solutions.
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    EFFECT OF ZINC OXIDE NANOPARTICLES AGAINST PHYTOPHTHORA SPP. CAUSING DURIAN DISEASES IN VITRO
    (2024-01-01)
    Keeratirawee, Kanchalar
    ;
    Kongtragoul, Pornprapa
    ;
    Pantakan, Sansanee
    The efficiency of zinc oxide nanoparticles (ZnO-NPs) in inhibiting Phytophthora spp. growth in both durian fruits and stems is demonstrated. The size of ZnO-NPs used was in range of 25-50 nm in diameter. Diseased durian sample were collected from a durian orchard located in southern of Thailand. The pathogen of diseased durian was isolated for subsequent morphological identification. Three distinct strains of Phytophthora spp. (designated as PHY41, PHY45, PHY49) were successfully isolated and were found in colonies with rings and stellate pattern, ovoid, limoniform sporangium with semi-papillate or papillate, and formation chlamydospores. All isolates were exhibiting 100% pathogenicity on durian, as indicated by the disease incidence (DI). The concentration of ZnO-NPs played a crucial role in suppressing the growth of Phytophthora spp. in all isolates. Higher concentration of ZnO-NPs resulted in increased inhibition of growth. ZnO-NPs at the concentration of 2000 µg/mL effectively suppressed the mycelial growth of all fungi isolates at 57.76-69.84%. Additionally, ZnO-NPs at the concentration of 500 µg/mL completely inhibited the sporulation only in the PHY49 strains. Furthermore, ZnO-NPs concentration ranging from 1000-2000 µg/mL resulted in completed inhibition in sporulation in all Phytophthora spp. isolates. The result demonstrated that ZnO-NPs at all concentrations effectively delayed the germination of Phytophthora spp.