Todhanakasem, Tatsaporn

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Todhanakasem, Tatsaporn
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tatsaporn.to@kmitl.ac.th

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    Item type:Publication,
    Development of Bioactive Opuntia ficus-indica Edible Films Containing Probiotics as a Coating for Fresh-Cut Fruit
    (2022-11-01)
    Todhanakasem, Tatsaporn
    ;
    Boonchuai, Pratana
    ;
    Itsarangkoon Na Ayutthaya, Pavarunya
    ;
    Suwapanich, Rachit
    ;
    Hararak, Bongkot
    Bioactive edible films have received more attention in recent years as a method for food preservation with value-added functions. The aim of this study was to develop a bioactive edible film containing mucilage of cactus (Opuntia ficus-indica) and incorporating the probiotic strain Enterococcus faecium FM11-2 as an active component to promote consumer health benefits. Opuntia ficus-indica is rich in nutritional and bioactive compounds and the abundance of this cactus makes it attractive for food applications. Mucilage of Opuntia ficus-indica contained 0.47 ± 0.06 mg/g total sugar, 0.33 ± 0.06 mg AGE/mL phenolic content, 0.14 mg/ mL vitamin C, and possessed 35.51 ± 1.88% DPPH scavenging activity. The edible film that was developed exhibited the following characteristics: thickness of 0.02–0.11 mm, percent moisture content 0.19–0.24%, water solubility 30.66–59.41% and water vapor permeability of 0.15–1.5 g·mm/m<sup>2</sup>·min·kpa, while the range of the variation depended on the type of plasticizer used (either sorbitol or glycerol). The addition of sorbitol in the film provided the maximum mechanical strength based on the evaluation of tensile strength, Young’s modulus and elongation at break (44.71 ± 0.78 MPa, 113.22 ± 0.23 MPa and 39.47 ± 0.61%, respectively). The optimal formulation of the edible film, according to the physicochemical, physical and maintenance of fresh-cut apple slices, contained cactus mucilage, gelatin, glycerol and a probiotic. The incorporation of a probiotic into the cactus film created a bioactive edible film that could provide a health benefit. While improvement is needed to maintain the survival rate of the probiotic, this work presents an exciting method for furthering the study of food preservation with edible films.
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    Engineering Zymomonas mobilis for improving genetic transformation and stability of multi-gene biosynthetic pathways
    (2025-12-01)
    Huang, Yuhuan
    ;
    Wang, Xiaojie
    ;
    Chen, Mao
    ;
    Wu, Yanran
    ;
    Todhanakasem, Tatsaporn
    Zymomonas mobilis holds significant promise for metabolic engineering but suffers from inefficient transformation and instability of plasmids over 8 kb. In this study, an element library containing promoters and terminators was constructed by analyzing the promoter activities of Z. mobilis ZM4. Using these regulatory elements, the β-carotene gene cluster (crtEXYIB) and individual genes (crtE, crtXYI, crtB) were regulated to construct pEZ-crt1 and pEZ-crt2 plasmid series. Screening identified top-yielding strains CRT1-29 (0.93 mg/g DCW) and CRT2-22 (1.25 mg/g DCW). Additionally, three key genes determining both the genetic transformation and stability of exogenous plasmids were identified by inactivating the genes encoding Restriction-Modification (R-M) or DNA-repair systems in Z. mobilis ZM4. The knockout of mrr and hsdM not only enhanced the transformation efficiency of a 3.95-kb methylated regular plasmid but also enabled the successful electroporation of a 9.5-kb reporter plasmid harboring a β-carotene expression cassette, which could not be transformed into Z. mobilis ZM4 and Mrr via electroporation previously. Moreover, the deletion of tatD prompted the stability of exogenous plasmid DNAs. The resultant triple-deficiency mutant MHT (Δmrr-hsdM-tatD) maintained the β-carotene expression plasmid in Z. mobilis, producing 2.09 mg/g DCW of β-carotene. Our study will prompt the application of Z. mobilis in metabolic engineering and synthetic biology.
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    Expanding the horizons of levan: from microbial biosynthesis to applications and advanced detection methods
    (2024-07-01)
    Wang, Sijie
    ;
    Wu, Bo
    ;
    Todhanakasem, Tatsaporn
    Levan, a β-(2,6)-linked fructose polymer, exhibits diverse properties that impart versatility, rendering it a highly sought-after biopolymer with various industrial applications. Levan can be produced by various microorganisms using sucrose, food industry byproducts and agricultural wastes. Microbial levan represents the most potent cost-effective process for commercial-scale levan production. This study reviews the optimization of levan production by understanding its biosynthesis, physicochemical properties and the fermentation process. In addition, genetic and protein engineering for its increased production and emerging methods for its detection are introduced and discussed. All of these comprehensive studies could serve as powerful tools to optimize levan production and broaden its applications across various industries.
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    Economic co-production of cellulosic ethanol and microalgal biomass through efficient fixation of fermentation carbon dioxide
    (2024-03-01)
    Liu, Linpei
    ;
    Zhou, Zheng
    ;
    Gong, Guiping
    ;
    Wu, Bo
    ;
    Todhanakasem, Tatsaporn
    An integrated process for the co-production of cellulosic ethanol and microalgal biomass by fixing CO<inf>2</inf> generated from bioethanol fermentation is proposed. Specifically, over one-fifth of the fermentative carbon was converted into high-purity CO<inf>2</inf> during ethanol production. The optimal concentration of 4 % CO<inf>2</inf> was identified for the growth and metabolism of Chlorella sp. BWY-1. A multiple short-term intermittent CO<inf>2</inf> supply system was established to efficiently fix and recycle the waste CO<inf>2</inf>. Using this system, economical co-production of cellulosic ethanol by Zymomonas mobilis and microalgal biomass in biogas slurry wastewater was achieved, resulting in the production of ethanol at a rate of 0.4 g/L/h and a fixed fermentation CO<inf>2</inf> of 3.1 g/L/d. Moreover, the amounts of algal biomass and chlorophyll a increased by over 50 % and two-fold, respectively. Through techno-economic analysis, the integrated process demonstrated its cost-effectiveness for cellulosic ethanol production. This study presents an innovative approach to a low-carbon circular bioeconomy.