Sriwatcharakul, Suttijit
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Preferred name
Sriwatcharakul, Suttijit
Alternative Name
Sriwatcharakul, S.
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Email
suttijit.sr@kmitl.ac.th
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Item type:Publication, Antimicrobial activities and phytochemicals of Murraya paniculata L. flowers, leaves and bark crude extracts(2023-01-01); ; Taveekijakarn, PraveenaAims: Murraya paniculata (L.) has been widely employed in medicine, has also been modified to serve as an ingredient in health foods and found application in cosmetics. This study was aimed to assess the biological activities of M. paniculata by analyzing the chemical compositions of its flowers, leaves and bark. Methodology and results: Crude extracts drawn from the flowers, leaves and bark of M. paniculata underwent testing to determine the antibacterial properties in terms of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), as well as the overall chemical composition, total phenolic content, flavonoids and antioxidant activity. Crude extract of leaves exhibited the most potent antibacterial activity when tested against Staphylococcus aureus TISTR 1466, Bacillus subtilis ATCC 6633 and Pseudomonas aeruginosa ATCC 27853. The crude extract from bark delivered the most significant antibacterial activity when tested against Micrococcus luteus TISTR 9341, Escherichia coli ATCC 1261, Pseudomonas sp., Streptococcus sp. and Methicilin resistant S. aureus (MRSA). For all crude extracts, the MIC value against M. luteus TISTR 9341 was 12.5 mg/mL. Meanwhile, the MBC value for the crude extract of leaves against B. subtilis ATCC 6633 was 12.5 mg/mL, whereas, for flower and bark crude extracts, the MBC value against S. aureus TISTR 1466 was 25 mg/mL. Antioxidant activity was at its highest for the crude extract from bark (IC50 = 1.36 mg/mL). The highest phenolic content was recorded for the crude extract from bark, while the highest flavonoid content came from the crude extract of leaves (70.81 ± 0.31 mgGAE/g extract and 115.73 ± 1.18 mgQE/g extract, respectively). Conclusion, significance and impact of study: The research findings suggest that the crude extracts of M. paniculata leaves and bark show greater significant levels of bioactivity than was the case for crude extracts from flowers. The research findings could help in exploring the possibilities of using M. paniculata for pharmaceutical purposes and in aquaculture. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Evaluation of bioactivities of Phyllanthus emblica seed(2020-02-01)This research aim to study bioactivity of seed extract compare with fruit extract of Indian gooseberry; Phyllanthus emblica that use as medicinal plant widely in Thailand. Analyzed the efficacy of free radical scavenging by using DPPH radical scavenging assay reveal that the highest antioxidant activity found in the highest concentration, 10 mg/ml and both fruit and seed extracts had non-significant percentage of reduction as α-tocopherol. The determination of phytochemical exhibited that seed extract had the higher contents of tannin and flavonoid than fruit extract as 126.71 mg TAE/g extract and 1016.25 mg QE/g extract, respectively. Cytotoxicity studied of extracts against human breast adenocarcinoma cell line by MTT assay at the maximum concentration 10000 μg/ml found that seed extract had more toxicity than fruit extract at 68.98% and 35.57%. According to the bioactivities results, the seed of Phyllanthus emblica has potential to replace the fruit for medical aspects to add value of this plant. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Major isoflavonoid contents of the 1-year-cultivated phytoestrogen-rich herb, Pueraria mirifica(2007-11-20)