Parinthawong, Nonglak

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Parinthawong, Nonglak
Alternative Name
Parinthawong, N.
Main Affiliation
Email
nonglak.pa@kmitl.ac.th

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    Item type:Publication,
    Development of novel InDel markers by whole-genome sequence comparison and genetic diversity assessment of Thailand rice blast fungus populations
    (2025-12-01)
    Thamkirati, Napassorn
    ;
    Suktrakul, Worrawit
    ;
    Ngernmuen, Athipat
    ;
    Toojinda, Theerayut
    ;
    Katengam, Sureeporn
    InDel markers are commonly used to assess genetic relationships among populations. In this study, we employed a whole-genome sequence comparison method to identify and develop InDel markers for the rice blast fungus Pyricularia oryzae. We analyzed 152 whole-genome sequences of P. oryzae isolates from diverse global regions, including Brazil, Burundi, China, Colombia, Côte d'Ivoire, France, Ghana, Hungary, India, Japan, Korea, Laos, Madagascar, Mali, Morocco, Nepal, the Philippines, Portugal, Spain, Suriname, Thailand, the UK, the USA, and Zambia. Our analysis identified a total of 233,595 InDel loci distributed across the seven chromosomes of P. oryzae. From these, 82 loci were selected based on their high polymorphism across the 152 genome sequences. The effectiveness of these 82 loci was assessed by analyzing the genetic diversity of 47 Thai rice blast isolates alongside two reference isolates, GUY11 (France) and KJ201 (Korea). Of the 82 InDel loci, 33 exhibited polymorphisms, with 2–4 alleles per locus and polymorphic information content (PIC) scores ranging from 0.04 to 0.67. Principal coordinate and structure analyses revealed two genetic subgroups among the Thai rice blast isolates, categorized according to host specificity. Genetic relationships highlighted disparities among rice blast populations based on their respective hosts: rice and grassy weeds. This finding suggests a correlation between genetic relatedness and the plant hosts susceptible to rice blast disease. The newly developed InDel markers provide a valuable resource for future research in this field.
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    Item type:Publication,
    Whole-Genome Sequencing Reveals SNP-Based Genetic Diversity and Population Structure of the Thai Rice Blast Fungus
    (2026-01-01)
    Longya, Apinya
    ;
    Suktrakul, Worrawit
    ;
    Suksirt, Mantira
    ;
    Korinsak, Siripar
    ;
    Pootakham, Wirulda
    Rice blast, caused by Magnaporthe oryzae, is a devastating disease threatening global rice production. While Southeast Asia is a centre of origin, the pathogen's population dynamics in Thailand are not well understood. We analysed whole-genome sequences from 191 M. oryzae isolates (60 from Thailand and 131 global) to investigate their genetic diversity, structure and pathogenicity. Phylogenomic analysis revealed that Thai isolates cluster with other Asian populations but exhibit greater within-population diversity, forming two major genetic clusters. We found that Thai rice isolates from rain-fed lowland and irrigated rice in the northeast and central regions cluster in World Group 3, while isolates from upland rice in northern Thailand cluster in World Group 1. This revised interpretation provides a more accurate representation of the population structure and highlights previously uncharacterised diversity. To link molecular data with phenotypes, we performed avirulence gene profiling and pathogenicity assays on 31 rice blast resistance near-isogenic lines. A correlation analysis showed a strong relationship for key gene pairs, including AvrPik and AvrPi9, highlighting their functional importance. In contrast, the correlation was low for Pii, Pizt and Pib due to the low frequency of their corresponding avirulence genes. The Avr-Pita interaction displayed a complex, haplotype-specific correlation. Our findings provide new insights into the genomic and pathogenic variation of M. oryzae in Thailand, establishing a robust link between avirulence gene profiles and observed pathogenicity phenotypes. This work offers a refined foundation for region-specific resistance breeding and disease management strategies.