Parinthawong, Nonglak

Preferred name

Alternative Name

Parinthawong, N.

Main Affiliation

School of Agricultural Technology

nonglak.pa@kmitl.ac.th

Now showing 1 - 10 of 15

Some of the metrics are blocked by your
consent settings
Development of novel InDel markers by whole-genome sequence comparison and genetic diversity assessment of Thailand rice blast fungus populations
(2025-12-01)
Thamkirati, Napassorn
;
Suktrakul, Worrawit
;
Ngernmuen, Athipat
;
Toojinda, Theerayut
;
Katengam, Sureeporn
InDel markers are commonly used to assess genetic relationships among populations. In this study, we employed a whole-genome sequence comparison method to identify and develop InDel markers for the rice blast fungus Pyricularia oryzae. We analyzed 152 whole-genome sequences of P. oryzae isolates from diverse global regions, including Brazil, Burundi, China, Colombia, Côte d'Ivoire, France, Ghana, Hungary, India, Japan, Korea, Laos, Madagascar, Mali, Morocco, Nepal, the Philippines, Portugal, Spain, Suriname, Thailand, the UK, the USA, and Zambia. Our analysis identified a total of 233,595 InDel loci distributed across the seven chromosomes of P. oryzae. From these, 82 loci were selected based on their high polymorphism across the 152 genome sequences. The effectiveness of these 82 loci was assessed by analyzing the genetic diversity of 47 Thai rice blast isolates alongside two reference isolates, GUY11 (France) and KJ201 (Korea). Of the 82 InDel loci, 33 exhibited polymorphisms, with 2–4 alleles per locus and polymorphic information content (PIC) scores ranging from 0.04 to 0.67. Principal coordinate and structure analyses revealed two genetic subgroups among the Thai rice blast isolates, categorized according to host specificity. Genetic relationships highlighted disparities among rice blast populations based on their respective hosts: rice and grassy weeds. This finding suggests a correlation between genetic relatedness and the plant hosts susceptible to rice blast disease. The newly developed InDel markers provide a valuable resource for future research in this field.
Some of the metrics are blocked by your
consent settings
Analysis of secondary metabolites and genes related to the pathogenicity of the rice blast fungus Pyricularia oryzae
(2022-09-01)
Pradapphai, P.
;
Kumlung, T.
;
Parinthawong, N.
Pyricularia oryzae causes rice blast disease and is one of the most significant plant pathogens worldwide. The rice blast fungus can produce a variety of phytotoxic secondary metabolites and has been found to be essential for host invasion. Two isolates, virulent isolate RBR55003 and non-virulent isolate CCO56003 were selected which based on their abilities to cause blast symptoms on rice leaves. Pyriculol and picolinic acid were detected in culture filtrate of both isolates during the exponential growth phase. Expression of genes involved in fungal pathogenicity and plant defense were analyzed. The expression of MoPks19 gene in isolate RBR55003 was higher than avirulent isolate CCO56003, indicating that MoPks19 may play a role in the pathogenesis of RBR55003. The expression of the OsWRKY30 gene that plays an important role in the regulation of a defense-related gene in rice was higher in CCO56003 inoculated than in RBR55003 inoculated rice sample, indicating the increased resistance of rice plants occurring in rice after non-virulent isolate infection, while the expression of the OsCPS4 gene involved in phytoalexin synthesis in rice was not different after isolate infections. Gene expression analysis revealed different responses according to plant-pathogen relationships.
Some of the metrics are blocked by your
consent settings
Morphological and Molecular Identification of Pythium spp. from Hydroponically-Grown Lettuce
(2022-01-01)
Talubnak, Chulalak
;
Schoonbeek, Henk Jan
;
Parinthawong, Nonglak
;
Jaenaksorn, Tanimnun
Herein, hydroponic Pythium communities in Thailand were investigated. The 38 Pythium isolates from asymptomatic and symptomatic lettuce roots were identified using morphological and molecular features. The data indicated that P. aphanidermatum and P. myriotylum were the predominant species. Regarding the rDNA-ITS study, all isolates were identified as P. aphanidermatum, P. myriotylum, P. deliense, or an unidentified Pythium species. The reconfirmation of the three unidentified Pythium isolates using the cytochrome oxidase subunit I (COI) gene in comparison to the representative isolates was achieved using P. aphanidermatum and P. myriotylum. The COI phylogenetic trees were similar to that of the ITS tree. Additionally, pathogenicity of the Pythium representative isolates to plant seeds was evaluated in a laboratory assay. The seedlings suffered serious symptoms from P. myriotylum SR31 infection, with 90-100% disease severity. The other isolates presented disease severity of less than 20% when compared with uninoculated control. This study provides a comprehensive identification of Pythium root rot in lettuce grown on hydroponics in Thailand and provides information on beneficial microorganisms and a resistance inducer in lettuce root rot.
Some of the metrics are blocked by your
consent settings
Blast resistance evaluation and genetic inheritance of gene controlling leaf blast resistance in Dawk Pa-yawm Rai variety (GS23774)
(2020-01-01)
Janthasri, S.
;
Parinthawong, N.
The landrace rice Dawk Pa-yawm Rai variety was highly resistant to 18 blast isolates, moderately resistant to 5 and susceptible to 2 blast isolates. Therefore, Dawk Pa-yawm Rai variety was broad-spectrum resistance to many blast isolates and can be used as genetic resource in breeding programs for blast disease resistance. The genetic inheritance of blast resistance in F<inf>2</inf> population was identified. Seven hundred and thirty F<inf>2</inf> plants from a cross between KDML105 (recipient) and Dawk Pa-yawm Rai (donor) were generated. The disease assessment revealed 524 F<inf>2</inf> plants were resistant and 206 F<inf>2</inf> plants were susceptible. The segregation of resistance and susceptible phenotypes showed a goodness of fit to the ratio 3:1 (R:S). The result found is important factor and useful in mapping of blast resistant gene in Dawk Pa-yawm Rai variety.
Some of the metrics are blocked by your
consent settings
Transformation RNA interference-mediated silencing of the Flavanone 3-Hydroxylase gene in Lotus (Nelumbo nucifera Geartn. cv. Buntharik)
(2020-07-01)
Saetiew, K.
;
Konee, C.
;
Parinthawong, N.
;
Arunyanart, S.
RNA interference mediated-silencing of the F3H gene in the lotus (Nelumbo nucifera Geartn.) cv. Buntharik inhibited the anthocyanin pathway after gene transformation. The plasmid pJA8F3H contained the inverted repeats of F3H fragments, bar gene as a selectable marker in plants, which is resistant to basta (glufosinate amonium). The transformation of particle bombardment was obtained with multiple shoots of lotus. F3H gene expression analysis showed that expression of F3H decreased in transgenic lotus when compared with the control. On the first and the second test, 5 transgenic lotus of the relative expression F3H gene was detected with 0.0496 and 0.466 times, respectively. The inhibition of gene expression decreased when they were cultured for a long time. The expression F3H gene was detected by RT-PCR, and real-time PCR resulted in consistent decreasing in the transgenic lotus compared with the non-transgenic lotus, which was able to inhibit the expression F3H gene.
Some of the metrics are blocked by your
consent settings
Non-Pathogenic Pythium Induced the Resistance in Hydroponic Lettuce Against Root Rot Disease
(2024-05-01)
Talubnak, Chulalak
;
Parinthawong, Nonglak
;
Schoonbeek, Henk Jan
;
Jaenaksorn, Tanimnun
Hydroponics is a common soilless type of culture. It is clean and gives high-yield crop production. Lettuce is the most widely grown leafy vegetable in hydroponics. The Pythium species causes root rot, a serious disease in lettuce. In this study, we investigated an effective elicitor to promote resistance in lettuce after pathogen infection. To evaluate the plant defense gene response profile, the expression stability of five candidate genes was studied using RT-qPCR. The LsPR1 and LsLTC2 gene expression were higher in lettuce leaves treated with non-pathogenic Pythium PyASR23 than in roots compared to untreated plants. To assess the disease, the rate of relative gene expression of LsLTC2, LsPR-1b like, LsERF, LsAOS, and LsDEF was associated with Pythium root rot pathogenesis. As a result, it was revealed that alterations in these gene expressions were a defense response to Pythium infection, which increased after pre-treatment with various elicitors for 24 hours. When the chemically stimulated lettuce was infected with pathogenic PySR31, the expression of these genes increased dramatically. These results suggested that non-pathogenic PyASR23 might be employed as an elicitor in lettuce roots and contribute to disease control by activating plant defense gene expression against the Pythium pathogen.
Some of the metrics are blocked by your
consent settings
Classification and diversity assessment of Pyricularia oryzae based on leaf and neck blast pathogenesis on Khao Dawk Mali 105
(2020-01-01)
Pradapphai, P.
;
Parinthawong, N.
Forty isolates of P. oryzae were obtained from disease outbreak areas in various regions of Thailand. The fungi were divided into two groups; group 1 included 2 isolates which cause only neck blast with disease score of 7 – 9 and group 2 included 23 isolates which cause leaf and neck blast symptoms with disease scores of 5 – 6 and 7 – 9, respectively. The two groups of fungi were tested on 31 NILs. The avirulence genes including Avr-Pik (Avr-Pik, Avr-Pikm, Avr-Pikp), Avr-Pi1, Avr-Pi3, Avr-Pi5(t), Avr-Pi7(t), Avr-Pi9, Avr-Pi20, Avr-Pii and Avr-Pita were found in both groups whereas Avr-Pi11, Avr-Pi12, Avr-Pi19, Avr-Pia, Avr-Pib, Avr-Pikh, Avr-Piks, Avr-Pish, Avr-Pita, Avr-Piz, Avr-Piz-5 and Avr-Piz-t were detected only in the group 2. The results explained the relationship between fungi and the avirulence genes functioned at different stages of rice.
Some of the metrics are blocked by your
consent settings
High nucleotide sequence variation of avirulent gene, AVR-Pita1, in Thai rice blast fungus population
(2020-12-01)
Damchuay, Katanyutita
;
Longya, Apinya
;
Sriwongchai, Tanee
;
Songkumarn, Pattavipha
;
Parinthawong, Nonglak
Rice blast disease, caused by Magnaporthe oryzae, is one of the most importance diseases of rice production worldwide. The key role of defense mechanism to combat this fungus in rice follows the gene-for-gene concept, which a plant resistant (R) gene product recognizes a fungal avirulent (AVR) effector and triggers the hypersensitive response. However, the AVR genes have been shown to be rapidly evolving resulting in high level of genetic diversity. The aims of this study were to examine the nucleotide sequence variation of AVR-Pita1 gene in Thai rice blast isolates and to identify the severity of blast disease using isogenic line of Pita gene. Seventy-six rice blast isolates collected from different parts of Thailand were used. Gene specific primers for AVR-Pita1 gene coding sequence were designed and used for identifying the genetic diversity of AVR-Pita1 gene by PCR amplification and sequencing. The obtained sequences were analysed for genetic variation and genetic relationship. Our results revealed the association between the sequence variations of AVR-Pita1 and selective forces from Pita gene. This phenomenon demonstrated the coevolution between rice blast resistant gene in rice and avirulent gene in blast fungus. The information about variation and evolutionary mechanisms of AVR gene obtained from this study can be used in rice blast resistant breeding programme.
Some of the metrics are blocked by your
consent settings
Protein hydrolysates from agricultural wastes for plant bacterial disease control
(2022-03-01)
Ditsawanon, T.
;
Parinthawong, N.
;
Phaonakrob, N.
;
Roytrakul, S.
Agricultural wastes, agro-industrial wastes and fishery wastes were collected and the protein hydrolysates were obtained with pepsin. Antibacterial activity of smaller than 3 kDa protein hydrolysates was determined against the plant pathogenic bacteria; Xanthomonas citri, Ralstonia solanacearum, Burkholderia cepacia and also against plant growth promoting rhizobacteria (PGPRs); Bacillus subtilis, Pseudomonas aeruginosa and P. fluorescens. Coconut residues (agro-industrial waste from coconut milk production), peanut seed coat (from peanut-based snack production) and rice straw (waste from rice farms) showed antimicrobial activity against X. citri, R. solanacearum and B. cepacia with higher than 74% inhibition. Coconut residue also increased growth of PGPRs, B. subtilis and P. fluorescens. Further protein hydrolysates from Nile tilapia (Oreochromis niloticus) and snake-head fish (Clarias batrachus) fin increased growth of all PGPRs.
Some of the metrics are blocked by your
consent settings
Genetic variation of avirulence genes (AVR-Pi9, AVR-Pik, AVR-Pita1) and genetic diversity of rice blast fungus, Pyricularia oryzae, in Thailand
(2022-02-01)
Sutthiphai, Thanathip
;
Damchuay, Katanyutita
;
Neupane, Ram Chandra
;
Longya, Apinya
;
Sriwongchai, Tanee
Genetic variation of the rice blast (Pyricularia oryzae) population in Thailand was investigated based on the nucleotide sequence of three avirulence genes, AVR-Pi9, AVR-Pik, and AVR-Pita1. Sixty rice blast isolates were collected from rice-growing areas around Thailand. Gene-specific primers were used to amplify these AVR genes and AVR-Pi9, AVR-Pik, and AVR-Pita1 were detected in 60, 57, and 23 isolates, respectively. Based on the AVR-Pi9 sequences, we identified one rice blast isolate containing an amino acid change from glutamic acid to aspartic acid. Moreover, two rice blast isolates had identical sequences to the rice blast strain originating in Japan, indicating a potential movement of this isolate from Japan to Thailand. Three AVR-Pik alleles were found, including AVR-PikA (3.51%), AVR-PikD (71.93%), and isolates with two copies of AVR-PikD and AVR-PikF (24.56%). AVR-PikA and AVR-PikF are virulent to Thai rice variety Jao Hom Nin. Six haplotypes of AVR-Pita1 were identified with one deletion and 12 amino acid substitutions. This study revealed that different AVR genes in Thai rice blast populations have different levels of genetic variation: AVR-Pi9 and AVR-Pik genes have a relatively low genetic diversity, while the AVR-Pita1 gene has high genetic diversity. We found AVR-Pi9 was not under selection pressure, while AVR-Pita1 was under purifying selection pressure. In addition, geographic location has influenced the distribution of genetic variation of AVR-Pita1. The information obtained from this study is valuable for the future development of breeding strategies for rice blast resistance in Thailand.

Parinthawong, Nonglak

Filters

Settings

Sort By

Results per page