Ochaikul, Duangjail

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Ochaikul, Duangjail
Alternative Name
Ochaikul, D.
Ochaikul, Duangjai
Ochaikul, Daungjai
Email
daungjai.oc@kmitl.ac.th

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    Bioethanol production from cassava starch using co-culture of saccharolytic molds with Saccharomyces cerevisiae TISTR 5088
    (2024-08-01)
    Pimpisai, Timakorn
    ;
    Maneerattanarungroj, Cherdsak
    ;
    Kingkaew, Engkarat
    ;
    Ochaikul, Duangjai
    Cassava serves as a cornerstone for sustainable bioethanol production in Thailand. The cassava root is highly valuable, comprising 50–70% starch on a dry weight basis. Starch can be converted into fermentable sugars through chemical or biological methods. This study focused on harnessing the enzymatic potential of saccharolytic molds, specifically Aspergillus oryzae TISTR 3086 or Amylomyces rouxii TISTR 3182, to facilitate saccharification of cassava starch, followed by fermentation using Saccharomyces cerevisiae TISTR 5088 for bioethanol production. Among the various configurations, the co-culture of Amy. rouxii TISTR 3182 with S. cerevisiae TISTR 5088 was the most effective, resulting in the highest ethanol yield. This finding was observed for both the separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes. Impressively, the SSF approach to ethanol productivity demonstrated superior results with an ethanol concentration of 25.4±0.3 g/l and an ethanol productivity of 0.53 g/l/h (yield of 1.40 g/g reducing sugars), surpassing the SHF approach (25.4±0.7 g/l, an ethanol productivity of 0.26 g/l/h, yield of 0.57 g/g reducing sugars) while also reducing the fermentation period. Further investigations into optimizing the conditions for ethanol production were carried out using a co-culture of Amy. rouxii TISTR 3182 with S. cerevisiae TISTR 5088 during SSF. This exploration revealed that employing 100 g/l cassava starch and initiating fermentation with a medium pH of 6.0 led to the highest ethanol concentration at 48 h. This process showed potential for ethanol production, harnessing the synergistic action of saccharolytic molds alongside yeast.
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    ENHANCING SUGAR RECOVERY FROM SUGARCANE BAGASSE: OPTIMIZING MICROWAVE-ASSISTED ALKALINE PRETREATMENT METHODS FOR BIOBASED ENERGY
    (2025-01-01)
    Kingkaew, Engkarat
    ;
    Tanasupawat, Somboon
    ;
    Phuengjayaem, Sukanya
    ;
    Poothong, Saranporn
    ;
    Ochaikul, Duangjai
    This study optimized microwave-assisted alkaline pretreatment for enhancing sugar recovery from sugarcane bagasse. Bagasse was dried, ground, and sieved to a uniform particle size before undergoing pretreatment with sodium hydroxide (NaOH). The effects of microwave power, duration, and NaOH concentration were investigated. Optimal conditions were identified as 800 W microwave power, 8% (w/v) NaOH, and a 2-minute duration (18.69 ± 0.05 g/L). An NaOH concentration of 8% (w/v) achieved high sugar recovery while minimizing chemical use, highlighting the balance between efficiency and sustainability. These findings provide a foundation for improving lignocellulosic biomass pretreatment for biofuel and biochemical production.
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    Functional and genomic characterization of Pediococcus pentosaceus NR4-4 reveals a safe bacteriocinogenic probiotic with strong anti-listerial activity and prebiotic metabolism
    (2026-05-01)
    Ratthanachot, Natnarin
    ;
    Woraprayote, Weerapong
    ;
    Janyaphisan, Thitiphorn
    ;
    Tanasupawat, Somboon
    ;
    Ochaikul, Duangjai
    The growing interest in bacteriocin-producing lactic acid bacteria from traditional fermented foods has highlighted the value of strains that combine antimicrobial activity, probiotic functionality, and genome-supported safety. This study presents an integrated functional and probiogenomic characterization of Pediococcus pentosaceus NR4-4 isolated from Thai fermented mustard greens. The strain was found to produce a heat-stable class IIa bacteriocin with pronounced inhibitory activity against Listeria monocytogenes . In addition, NR4-4 exhibited strong tolerance to simulated gastrointestinal conditions and demonstrated effective adhesion to Caco-2 intestinal epithelial cells. In vitro safety evaluations confirmed that the strain was non-hemolytic and did not exhibit cytotoxic effects toward AGS or Caco-2 cell lines. Whole-genome sequencing verified species identity and revealed the presence of pediocin-related gene clusters, carbohydrate-active enzymes associated with fructooligosaccharide utilization, and a favorable genome-based safety profile. Notably, no virulence factors, biogenic amine biosynthesis pathways, plasmids, or transferable antimicrobial resistance determinants were detected. The preferential metabolism of fructooligosaccharides further suggests compatibility with synbiotic formulations. Overall, these findings indicate that P. pentosaceus NR4-4 is a genomically supported, safe, and multifunctional probiotic candidate with potential applications in food biopreservation and functional food development.