Ochaikul, Duangjail

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Ochaikul, Duangjail
Alternative Name
Ochaikul, D.
Ochaikul, Duangjai
Ochaikul, Daungjai
Email
daungjai.oc@kmitl.ac.th

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    Item type:Publication,
    ENHANCING SUGAR RECOVERY FROM SUGARCANE BAGASSE: OPTIMIZING MICROWAVE-ASSISTED ALKALINE PRETREATMENT METHODS FOR BIOBASED ENERGY
    (2025-01-01)
    Kingkaew, Engkarat
    ;
    Tanasupawat, Somboon
    ;
    Phuengjayaem, Sukanya
    ;
    Poothong, Saranporn
    ;
    Ochaikul, Duangjai
    This study optimized microwave-assisted alkaline pretreatment for enhancing sugar recovery from sugarcane bagasse. Bagasse was dried, ground, and sieved to a uniform particle size before undergoing pretreatment with sodium hydroxide (NaOH). The effects of microwave power, duration, and NaOH concentration were investigated. Optimal conditions were identified as 800 W microwave power, 8% (w/v) NaOH, and a 2-minute duration (18.69 ± 0.05 g/L). An NaOH concentration of 8% (w/v) achieved high sugar recovery while minimizing chemical use, highlighting the balance between efficiency and sustainability. These findings provide a foundation for improving lignocellulosic biomass pretreatment for biofuel and biochemical production.
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    Item type:Publication,
    Functional and genomic characterization of Pediococcus pentosaceus NR4-4 reveals a safe bacteriocinogenic probiotic with strong anti-listerial activity and prebiotic metabolism
    (2026-05-01)
    Ratthanachot, Natnarin
    ;
    Woraprayote, Weerapong
    ;
    Janyaphisan, Thitiphorn
    ;
    Tanasupawat, Somboon
    ;
    Ochaikul, Duangjai
    The growing interest in bacteriocin-producing lactic acid bacteria from traditional fermented foods has highlighted the value of strains that combine antimicrobial activity, probiotic functionality, and genome-supported safety. This study presents an integrated functional and probiogenomic characterization of Pediococcus pentosaceus NR4-4 isolated from Thai fermented mustard greens. The strain was found to produce a heat-stable class IIa bacteriocin with pronounced inhibitory activity against Listeria monocytogenes . In addition, NR4-4 exhibited strong tolerance to simulated gastrointestinal conditions and demonstrated effective adhesion to Caco-2 intestinal epithelial cells. In vitro safety evaluations confirmed that the strain was non-hemolytic and did not exhibit cytotoxic effects toward AGS or Caco-2 cell lines. Whole-genome sequencing verified species identity and revealed the presence of pediocin-related gene clusters, carbohydrate-active enzymes associated with fructooligosaccharide utilization, and a favorable genome-based safety profile. Notably, no virulence factors, biogenic amine biosynthesis pathways, plasmids, or transferable antimicrobial resistance determinants were detected. The preferential metabolism of fructooligosaccharides further suggests compatibility with synbiotic formulations. Overall, these findings indicate that P. pentosaceus NR4-4 is a genomically supported, safe, and multifunctional probiotic candidate with potential applications in food biopreservation and functional food development.