Ochaikul, Duangjail

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Ochaikul, Duangjail
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Ochaikul, D.
Ochaikul, Duangjai
Ochaikul, Daungjai
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daungjai.oc@kmitl.ac.th

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    Item type:Publication,
    Optimization of bacterial cellulose production from wastewater of noodle processing by komagataeibacter sp. Pap1 and bio-cellulose paper production
    (2020-11-01)
    Sutthiphatkul, Tanyarat
    ;
    Suwanposri, Amornrat
    ;
    Ochaikul, Duangjai
    Bacterial Cellulose (BC) production from Fermented Rice Noodle Wastewater (FRNW) was fermented by Komagataeibacter sp. PAP1. In order to increase the production of BC, the FRNW-based medium was prepared and optimized for the cultivation of this bacterium. The optimized FRNW-based medium was composed of 5 % (w/v) mannitol, 0.1 % (w/v) beef extract, 0.5 % (v/v) ethanol, 1 % (v/v) acetic acid, pH 7.0 which were incubated at 30 °C for 10 days. Under these conditions, BC yielded 11.76 ± 0.34 g/L higher (4.40 fold) than the standard Hestrin-Schramm (HS) medium. The study on growth and BC production by Komagataeibacter sp. PAP1 in optimized culture condition showed that BC production by Komagataeibacter sp. PAP1 was growth-associated. The bacterial cell of Komagataeibacter sp. PAP1 increased exponentially from the 3<sup>rd</sup> to 5<sup>th</sup> day. The BC paper sheets produced using the obtained BC pellicle from an optimized FRNW-based medium gave higher mechanical strength than those from standard HS medium. This study reveals the use of FRNW as a substrate for BC production. The results indicated that FRNW, which is more environmentally friendly, can be used as an alternative low-cost substrate for BC production.