Ochaikul, Duangjail

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Ochaikul, Duangjail
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Ochaikul, D.
Ochaikul, Duangjai
Ochaikul, Daungjai
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daungjai.oc@kmitl.ac.th

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    Optimization of bacterial cellulose production from wastewater of noodle processing by komagataeibacter sp. Pap1 and bio-cellulose paper production
    (2020-11-01)
    Sutthiphatkul, Tanyarat
    ;
    Suwanposri, Amornrat
    ;
    Ochaikul, Duangjai
    Bacterial Cellulose (BC) production from Fermented Rice Noodle Wastewater (FRNW) was fermented by Komagataeibacter sp. PAP1. In order to increase the production of BC, the FRNW-based medium was prepared and optimized for the cultivation of this bacterium. The optimized FRNW-based medium was composed of 5 % (w/v) mannitol, 0.1 % (w/v) beef extract, 0.5 % (v/v) ethanol, 1 % (v/v) acetic acid, pH 7.0 which were incubated at 30 °C for 10 days. Under these conditions, BC yielded 11.76 ± 0.34 g/L higher (4.40 fold) than the standard Hestrin-Schramm (HS) medium. The study on growth and BC production by Komagataeibacter sp. PAP1 in optimized culture condition showed that BC production by Komagataeibacter sp. PAP1 was growth-associated. The bacterial cell of Komagataeibacter sp. PAP1 increased exponentially from the 3<sup>rd</sup> to 5<sup>th</sup> day. The BC paper sheets produced using the obtained BC pellicle from an optimized FRNW-based medium gave higher mechanical strength than those from standard HS medium. This study reveals the use of FRNW as a substrate for BC production. The results indicated that FRNW, which is more environmentally friendly, can be used as an alternative low-cost substrate for BC production.
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    Statistical optimisation of culture conditions for biocellulose production by Komagataeibacter sp. PAP1 using soya bean whey
    (2014-02-18)
    Suwanposri, Amornrat
    ;
    Yukphan, Pattaraporn
    ;
    Yamada, Yuzo
    ;
    Ochaikul, Duangjai
    A low-cost medium based on soya bean whey (SBW) was prepared as a by-product from agriculture and optimised for biocellulose (BC) production by Komagataeibacter sp. PAP1. The optimal conditions for BC production are: pH 6.21, 1.61% ethanol concentration (v/v) and 28.4°C. The use of optimised medium based on SBW increased BC production 3.6 fold compared to standard Hestrin-Schramm (HS) medium. The BC film produced from the optimised medium was stronger and more impermeable to water vapour and oxygen compared to the one produced from the standard HS medium. These properties allow SBW film to be developed into eco-friendly food packaging for oxygen-sensitive products. Its high water absorption capacity allows its use in biomedical application such as wound dressing. Our results demonstrate that SBW can be used as an alternative low-cost substrate for BC production on commercial scale. © 2014 by Maejo University, San Sai, Chiang Mai, 50290 Thailand.
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    Identification and biocellulose production of Gluconacetobacter strains isolated from tropical fruits in thailand
    (2013-03-28)
    Suwanposri, Amornrat
    ;
    Yukphan, Pattaraporn
    ;
    Yamada, Yuzo
    ;
    Ochaikul, Daungjai
    Two hundred and four strains of biocellulose (BC)-producing Gluconacetobacter strains were isolated from 48 rotten tropical fruits collected in Thailand. Twenty-nine representative isolates were selected from each of the 16 isolation sources and identified by morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis. The selected 29 isolates were divided into seven subgroups within the Gluconacetobacter xylinus group of the genus Gluconacetobacter and identified as Gluconacetobacter oboediens (subgroup I, five isolates), Gluconacetobacter rhaeticus (subgroup II, one isolate), Gluconacetobacter hansenii (subgroup III, seven isolates), Gluconacetobacter swingsii (subgroup IV, two isolates) and Gluconacetobacter sucrofermentans (subgroup V, two isolates). The remaining isolates were grouped into subgroups VIa (three isolates) and VIb (nine isolates). All the isolates were cultured in Hestrin-Schramm (HS) medium statically at 30°C for 7 days to determine cellulose production capability. Of the 29 isolates, isolate PAP1 (subgroup VIb, unidentified) gave the highest yield (1.15 g/L) of BC. However, the BC yield increased threefold (3.5 g/L) when D-glucose in HS medium was replaced by D-mannitol. © 2013 by Maejo University, San Sai, Chiang Mai, 50290 Thailand. Reproduction is permitted for noncommercial purposes.